Potential of Aqueous Humor as a Surrogate Tumor Biopsy for Retinoblastoma.
Berry JL, Xu L, Murphree AL, Krishnan S, Stachelek K, Zolfaghari E, McGovern K, Lee TC, Carlsson A, Kuhn P, Kim JW, Cobrinik D, Hicks J. Potential of Aqueous Humor as a Surrogate Tumor Biopsy for Retinoblastoma. JAMA Ophthalmology 2017, 135: 1221-1230. PMID: 29049475, PMCID: PMC5710399, DOI: 10.1001/jamaophthalmol.2017.4097.Peer-Reviewed Original ResearchConceptsTumor-derived DNACell-free DNARB eyesIntravitreous injectionAqueous humorAH samplesCopy number variationsSalvage therapyRB1 mutationsTumor biopsiesNumber variationsTertiary care hospitalCase series studyDNA copy number variationsDNA copy number alterationsCopy number variation profilesHigh-sensitivity kitChromosomal copy number variationsTumor DNA fractionPolymerase chain reaction primersWhole-genome sequencingPrimary enucleationCare hospitalDisease courseNumber variation profilesNot All Seeds Are Created Equal: Seed Classification Is Predictive of Outcomes in Retinoblastoma
Berry JL, Bechtold M, Shah S, Zolfaghari E, Reid M, Jubran R, Kim JW. Not All Seeds Are Created Equal: Seed Classification Is Predictive of Outcomes in Retinoblastoma. Ophthalmology 2017, 124: 1817-1825. PMID: 28655537, DOI: 10.1016/j.ophtha.2017.05.034.Peer-Reviewed Original ResearchMeSH KeywordsAntineoplastic Agents, AlkylatingAntineoplastic Combined Chemotherapy ProtocolsCarboplatinChildChild, PreschoolCryotherapyEtoposideFemaleFollow-Up StudiesHumansInfantInfusions, IntravenousIntravitreal InjectionsLaser CoagulationMaleMelphalanNeoplasm SeedingRetinal NeoplasmsRetinoblastomaRetrospective StudiesSalvage TherapyVincristineVitreous BodyConceptsIntra-arterial chemotherapyIntravitreal melphalanIntravenous chemotherapyVitreous seedingSalvage rateRetrospective reviewIVM injectionsOverall globe salvage ratePrimary intra-arterial chemotherapyGlobe salvage rateNonrandomized retrospective reviewOutcome of retinoblastomaRecurrent vitreous seedingPrimary outcome measureActive retinoblastomaVitreous seedsGlobe salvageIntravitreal chemotherapySingle centerIntravitreal injectionMore injectionsGrade 3Outcome measuresSecondary measuresEffective treatment