1998
Properties of an Inwardly Rectifying ATP-sensitive K+ Channel in the Basolateral Membrane of Renal Proximal Tubule
Mauerer U, Boulpaep E, Segal A. Properties of an Inwardly Rectifying ATP-sensitive K+ Channel in the Basolateral Membrane of Renal Proximal Tubule. The Journal Of General Physiology 1998, 111: 139-160. PMID: 9417141, PMCID: PMC1887768, DOI: 10.1085/jgp.111.1.139.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAmbystomaAnimalsBariumBiological TransportCationsCells, CulturedCytidine TriphosphateDiazoxideDiureticsElectric ConductivityGlyburideGuanosine TriphosphateHypoglycemic AgentsInosine TriphosphateIon Channel GatingKidney Tubules, ProximalKineticsMicrovilliPatch-Clamp TechniquesPotassium ChannelsSodium Chloride Symporter InhibitorsSodium-Potassium-Exchanging ATPaseThalliumThymine NucleotidesUridine TriphosphateConceptsProximal tubule cellsProximal tubulesTubule cellsBasolateral membraneChannel opener diazoxideChannel activityPump-leak couplingRenal proximal tubulesPivotal physiological rolesOpener diazoxideMillimolar dosesMM ATPHigh dosesPotassium conductanceCell membrane potentialInternal Mg2Cytosolic additionATPase pumpRegulation of an Inwardly Rectifying ATP-sensitive K+ Channel in the Basolateral Membrane of Renal Proximal Tubule
Mauerer U, Boulpaep E, Segal A. Regulation of an Inwardly Rectifying ATP-sensitive K+ Channel in the Basolateral Membrane of Renal Proximal Tubule. The Journal Of General Physiology 1998, 111: 161-180. PMID: 9417142, PMCID: PMC1887764, DOI: 10.1085/jgp.111.1.161.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAmbystomaAnimalsBiological TransportCalciumCell MembraneColforsinCyclic AMP-Dependent Protein KinasesEnzyme ActivationEpithelial CellsHydrogen-Ion ConcentrationIon Channel GatingKidney Tubules, ProximalPatch-Clamp TechniquesPhosphorylationPotassium ChannelsProtein Kinase CSodium-Potassium-Exchanging ATPaseConceptsBasolateral membraneProtein kinase AActin cytoskeletonATPase pumpOuter membraneProtein kinaseKinase ACellular metabolismKATP channelsSecond messengerStimulation of transportTransport activityPhysiological importanceMembrane stretchPhorbol esterChannel activityMembrane patchesRegulationIntracellularO patchesFunctional couplingHypotonic swellingCell-attached patchesCell depolarizationRenal proximal tubules
1997
The electrogenic Na/HCO3 cotransporter.
Boron W, Fong P, Hediger M, Boulpaep E, Romero M. The electrogenic Na/HCO3 cotransporter. Wiener Klinische Wochenschrift 1997, 109: 445-56. PMID: 9261985.Peer-Reviewed Original ResearchMeSH KeywordsAcid-Base EquilibriumAnimalsCarrier ProteinsCloning, MolecularKidney Tubules, ProximalRabbitsRatsRNA, MessengerSodium-Bicarbonate SymportersUrodelaXenopus laevisConceptsElectrogenic Na/HCO3 cotransporterProximal tubulesHCO3- cotransporterRenal proximal tubulesCO2/10 mM HCO3HCO3- transportersRegulation of intracellularBasolateral membraneNumber of tissuesNative oocytesRapid hyperpolarizationXenopus laevis oocytesExtracellular ClBicarbonate cotransporterOocyte expressionCotransporterMM HCO3OocytesCell typesMost cellsMembrane potentialAmino acid homologySingle cloneWater-injected controlsTubulesThe Renal Electrogenic Na+:HCO3− Cotransporter
Boron W, Hediger M, Boulpaep E, Romero M. The Renal Electrogenic Na+:HCO3− Cotransporter. Journal Of Experimental Biology 1997, 200: 263-268. PMID: 9050234, DOI: 10.1242/jeb.200.2.263.Peer-Reviewed Original ResearchMeSH KeywordsAmbystomaAnimalsBicarbonatesCarrier ProteinsCloning, MolecularDNA, ComplementaryHydrogen-Ion ConcentrationKidney Tubules, ProximalMembrane PotentialsMicroinjectionsOocytesRabbitsRecombinant Fusion ProteinsRNA, MessengerSodiumSodium-Bicarbonate SymportersStructure-Activity RelationshipXenopus laevisConceptsRenal proximal tubules
1996
A novel preparation of dissociated renal proximal tubule cells that maintain epithelial polarity in suspension
Segal A, Boulpaep E, Maunsbach A. A novel preparation of dissociated renal proximal tubule cells that maintain epithelial polarity in suspension. American Journal Of Physiology 1996, 270: c1843-c1863. PMID: 8764170, DOI: 10.1152/ajpcell.1996.270.6.c1843.Peer-Reviewed Original ResearchConceptsEpithelial polarityFunctional polaritySingle cellsMembrane trafficking pathwaysCell surface polarityBasolateral membrane domainsIon channel proteinsBasolateral membraneApical brush borderCell polarityTrafficking pathwaysActin cytoskeletonMembrane domainsProximal tubule cellsMost epithelial cellsChannel proteinsZO-1 proteinTubule cellsJunctional complexesTight junctional complexesPolarized structureSingle cell modelEpithelial cellsRenal proximal tubule cellsZO-1
1990
Bicarbonate transport mechanisms in the Ambystoma kidney proximal tubule: transepithelial potential measurements.
Bock J, Boulpaep E. Bicarbonate transport mechanisms in the Ambystoma kidney proximal tubule: transepithelial potential measurements. The Yale Journal Of Biology And Medicine 1990, 63: 529-47. PMID: 2092412, PMCID: PMC2589409.Peer-Reviewed Original Research
1988
Cell membrane water permeabilities and streaming currents in Ambystoma proximal tubule
Tripathi S, Boulpaep E. Cell membrane water permeabilities and streaming currents in Ambystoma proximal tubule. American Journal Of Physiology 1988, 255: f188-f203. PMID: 3394810, DOI: 10.1152/ajprenal.1988.255.1.f188.Peer-Reviewed Original ResearchMeSH KeywordsAmbystomaAnimalsBody WaterCell Membrane PermeabilityElectrophysiologyFemaleKidney Tubules, ProximalReference Values
1987
pH-Dependent electrical properties and buffer permeability of theNecturus renal proximal tubule cell
Steels P, Boulpaep E. pH-Dependent electrical properties and buffer permeability of theNecturus renal proximal tubule cell. The Journal Of Membrane Biology 1987, 100: 165-182. PMID: 3430572, DOI: 10.1007/bf02209148.Peer-Reviewed Original ResearchUltrastructural changes in isolated perfused proximal tubules during osmotic water flow
Maunsbach A, Tripathi S, Boulpaep E. Ultrastructural changes in isolated perfused proximal tubules during osmotic water flow. American Journal Of Physiology 1987, 253: f1091-f1104. PMID: 3425719, DOI: 10.1152/ajprenal.1987.253.6.f1091.Peer-Reviewed Original ResearchIsolated perfused Ambystoma proximal tubule: hydrodynamics modulates ultrastructure
Tripathi S, Boulpaep E, Maunsbach A. Isolated perfused Ambystoma proximal tubule: hydrodynamics modulates ultrastructure. American Journal Of Physiology 1987, 252: f1129-f1147. PMID: 3591955, DOI: 10.1152/ajprenal.1987.252.6.f1129.Peer-Reviewed Original ResearchElectrochemical analysis of renal Na+-glucose cotransport in salamander proximal tubules
Morgunov N, Boulpaep E. Electrochemical analysis of renal Na+-glucose cotransport in salamander proximal tubules. American Journal Of Physiology 1987, 252: f154-f169. PMID: 3812699, DOI: 10.1152/ajprenal.1987.252.1.f154.Peer-Reviewed Original ResearchConceptsLuminal glucoseProximal tubulesFavorable chemical gradientFavorable electrical gradientSodium concentrationSodium-glucose cotransporterIntracellular sodium activityElectrogenic responseSodium activityMM D-glucoseMembrane potentialSalamander proximal tubuleLuminal perfusateOmega X cm2Glucose cotransportGlucose concentrationLuminal membranePhysiological rangeX cm2Luminal membrane potentialGlucoseD-glucoseTubulesLuminal membrane resistancePerfusate
1984
Quantitative ultrastructure and functional correlates in proximal tubule of Ambystoma and Necturus
Maunsbach A, Boulpaep E. Quantitative ultrastructure and functional correlates in proximal tubule of Ambystoma and Necturus. American Journal Of Physiology 1984, 246: f710-f724. PMID: 6720973, DOI: 10.1152/ajprenal.1984.246.5.f710.Peer-Reviewed Original ResearchMeSH KeywordsAmbystomaAnimalsCell MembraneEpitheliumFemaleKidney Tubules, ProximalMitochondriaNecturusConceptsBasal cell membraneCell membraneStructure-function correlationsIntercellular spacesBasal cell surfaceLateral cell membranesBasal extracellular labyrinthMorphometric comparative analysisVolume of cellsInterspecies variabilityCell surfaceAmbystomaQuantitative ultrastructureElaborate organizationBasolateral membraneLateral intercellular spacesSpeciesSalamander Ambystoma tigrinumExtracellular channelsMembraneUltrastructureAmbystoma tigrinumFunctional propertiesProximal tubules
1983
Rheogenic transport in the renal proximal tubule.
Sackin H, Boulpaep E. Rheogenic transport in the renal proximal tubule. The Journal Of General Physiology 1983, 82: 819-851. PMID: 6319539, PMCID: PMC2228722, DOI: 10.1085/jgp.82.6.819.Peer-Reviewed Original ResearchMeSH KeywordsAmbystomaAnimalsBiological TransportElectrophysiologyFemaleIon ChannelsKidney Tubules, ProximalMembrane PotentialsPostural BalanceParacellular shunt ultrastructure and changes in fluid transport in Necturus proximal tubule
Maunsbach A, Boulpaep E. Paracellular shunt ultrastructure and changes in fluid transport in Necturus proximal tubule. Kidney International 1983, 24: 610-619. PMID: 6663983, DOI: 10.1038/ki.1983.201.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiological Transport, ActiveExtracellular SpaceFemaleHydrostatic PressureKidney Tubules, ProximalMaleMicroscopy, ElectronNecturusSodiumChloride transport across the basolateral cell membrane of theNecturus proximal tubule: Dependence on bicarbonate and sodium
Guggino W, London R, Boulpaep E, Giebisch G. Chloride transport across the basolateral cell membrane of theNecturus proximal tubule: Dependence on bicarbonate and sodium. The Journal Of Membrane Biology 1983, 71: 227-240. PMID: 6302263, DOI: 10.1007/bf01875464.Peer-Reviewed Original ResearchConceptsBasolateral cell membraneProximal tubule cellsBasolateral solutionNH4Cl exposurePH-sensitive microelectrodesProximal tubulesTubule cellsPerfusion solutionCell membraneIntracellular chlorideTheNecturus proximal tubuleIntracellular pHApical cell membraneNH4Cl treatmentChloride transportChloride activityKidneyIntracellular pH regulation in the renal proximal tubule of the salamander. Basolateral HCO3- transport.
Boron W, Boulpaep E. Intracellular pH regulation in the renal proximal tubule of the salamander. Basolateral HCO3- transport. The Journal Of General Physiology 1983, 81: 53-94. PMID: 6833997, PMCID: PMC2215562, DOI: 10.1085/jgp.81.1.53.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBicarbonatesBiological TransportChloridesElectrophysiologyFemaleHydrogenHydrogen-Ion ConcentrationIntracellular MembranesKidney Tubules, ProximalSodiumUrodelaConceptsProximal tubulesRenal proximal tubulesRapid fallBasolateral HCO3- transportBasolateral membraneTiger salamander Ambystoma tigrinumReversible fallNominal absencePresence of HCO3Removal of ClTransient depolarizationSimilar reductionBasolateral membrane potentialSalamander Ambystoma tigrinumHCO3- transportHCO3- transportersMembrane potentialOnly minor effectsAforementioned changesTubulesCl-sensitive microelectrodesDepolarizationConstant PCO2Opposite effectAmbystoma tigrinumIntracellular pH regulation in the renal proximal tubule of the salamander. Na-H exchange.
Boron W, Boulpaep E. Intracellular pH regulation in the renal proximal tubule of the salamander. Na-H exchange. The Journal Of General Physiology 1983, 81: 29-52. PMID: 6833996, PMCID: PMC2215563, DOI: 10.1085/jgp.81.1.29.Peer-Reviewed Original ResearchMeSH KeywordsAmilorideAnimalsChloridesFemaleHydrogenHydrogen-Ion ConcentrationIntracellular MembranesKidney Tubules, ProximalQuaternary Ammonium CompoundsSodiumStilbenesUrodelaConceptsProximal tubulesProximal tubule cellsRenal proximal tubulesTiger salamander Ambystoma tigrinumPH-sensitive microelectrodesFree RingerMaximal effectTubule cellsTransient riseDiuretic amilorideBasolateral sideBasolateral solutionExponential time courseSalamander Ambystoma tigrinumTime courseCl removalMaximal rateBasolateral membraneNormal rateTubulesAmbystoma tigrinumPH regulationCellsActive transportRecovery
1982
Cellular and paracellular resistances of theNecturus proximal tubule
Guggino W, Windhager E, Boulpaep E, Giebisch G. Cellular and paracellular resistances of theNecturus proximal tubule. The Journal Of Membrane Biology 1982, 67: 143-154. PMID: 7097759, DOI: 10.1007/bf01868657.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell MembraneElectric ConductivityEpitheliumKidney Tubules, ProximalMembrane PotentialsMethodsMicroelectrodesNecturus maculosusOilsElectrical properties of chloride transport across theNecturus proximal tubule
Guggino W, Boulpaep E, Giebisch G. Electrical properties of chloride transport across theNecturus proximal tubule. The Journal Of Membrane Biology 1982, 65: 185-196. PMID: 7062339, DOI: 10.1007/bf01869962.Peer-Reviewed Original Research
1981
Isolated perfused salamander proximal tubule. II. Monovalent ion replacement and rheogenic transport
Sackin H, Boulpaep E. Isolated perfused salamander proximal tubule. II. Monovalent ion replacement and rheogenic transport. American Journal Of Physiology 1981, 241: f540-f555. PMID: 7304748, DOI: 10.1152/ajprenal.1981.241.5.f540.Peer-Reviewed Original Research