1998
Regulation of an Inwardly Rectifying ATP-sensitive K+ Channel in the Basolateral Membrane of Renal Proximal Tubule
Mauerer U, Boulpaep E, Segal A. Regulation of an Inwardly Rectifying ATP-sensitive K+ Channel in the Basolateral Membrane of Renal Proximal Tubule. The Journal Of General Physiology 1998, 111: 161-180. PMID: 9417142, PMCID: PMC1887764, DOI: 10.1085/jgp.111.1.161.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAmbystomaAnimalsBiological TransportCalciumCell MembraneColforsinCyclic AMP-Dependent Protein KinasesEnzyme ActivationEpithelial CellsHydrogen-Ion ConcentrationIon Channel GatingKidney Tubules, ProximalPatch-Clamp TechniquesPhosphorylationPotassium ChannelsProtein Kinase CSodium-Potassium-Exchanging ATPaseConceptsBasolateral membraneProtein kinase AActin cytoskeletonATPase pumpOuter membraneProtein kinaseKinase ACellular metabolismKATP channelsSecond messengerStimulation of transportTransport activityPhysiological importanceMembrane stretchPhorbol esterChannel activityMembrane patchesRegulationIntracellularO patchesFunctional couplingHypotonic swellingCell-attached patchesCell depolarizationRenal proximal tubules
1996
A novel preparation of dissociated renal proximal tubule cells that maintain epithelial polarity in suspension
Segal A, Boulpaep E, Maunsbach A. A novel preparation of dissociated renal proximal tubule cells that maintain epithelial polarity in suspension. American Journal Of Physiology 1996, 270: c1843-c1863. PMID: 8764170, DOI: 10.1152/ajpcell.1996.270.6.c1843.Peer-Reviewed Original ResearchConceptsEpithelial polarityFunctional polaritySingle cellsMembrane trafficking pathwaysCell surface polarityBasolateral membrane domainsIon channel proteinsBasolateral membraneApical brush borderCell polarityTrafficking pathwaysActin cytoskeletonMembrane domainsProximal tubule cellsMost epithelial cellsChannel proteinsZO-1 proteinTubule cellsJunctional complexesTight junctional complexesPolarized structureSingle cell modelEpithelial cellsRenal proximal tubule cellsZO-1
1993
Whole-cell currents in single and confluent M-1 mouse cortical collecting duct cells.
Korbmacher C, Segal A, Fejes-Tóth G, Giebisch G, Boulpaep E. Whole-cell currents in single and confluent M-1 mouse cortical collecting duct cells. The Journal Of General Physiology 1993, 102: 761-793. PMID: 8270912, PMCID: PMC2229168, DOI: 10.1085/jgp.102.4.761.Peer-Reviewed Original ResearchConceptsWhole-cell currentsWhole-cell conductanceConfluent cellsWhole-cell membrane currentsBasolateral K channelsCells 24 hK conductancePA/cellMicroM glibenclamideAmiloride-sensitive conductanceTransgenic miceReabsorb sodiumOutward currentsM amilorideGlibenclamide inhibitionDuct cellsPrincipal cellsMembrane currentsBath KLarge depolarizationChloride conductanceK channelsLipophilic drugsPermeable supportsMice