2004
Characteristics of renal Rhbg as an NH4+ transporter
Nakhoul N, DeJong H, Abdulnour-Nakhoul S, Boulpaep E, Hering-Smith K, Hamm L. Characteristics of renal Rhbg as an NH4+ transporter. American Journal Of Physiology. Renal Physiology 2004, 288: f170-f181. PMID: 15353405, DOI: 10.1152/ajprenal.00419.2003.Peer-Reviewed Original Research
1998
Transcellular Chloride Pathways in Ambystoma Proximal Tubule
Abdulnour-Nakhoul S, Boulpaep E. Transcellular Chloride Pathways in Ambystoma Proximal Tubule. The Journal Of Membrane Biology 1998, 166: 15-35. PMID: 9784583, DOI: 10.1007/s002329900444.Peer-Reviewed Original ResearchMeSH KeywordsAmbystomaAnimalsCell MembraneChloride ChannelsChloridesIon TransportMembrane PotentialsSodiumConceptsProximal tubulesChloride channel blockerContribution of transportersAmbystoma proximal tubuleTransepithelial potential differenceDisulfonic stilbeneCl-HCO3 exchangeFree RingerLines of evidenceTubulesBasolateral cell membraneLumenNa-independent ClMembrane potentialBasolateral membraneApical membrane potentialAiClBlockersRegulation of an Inwardly Rectifying ATP-sensitive K+ Channel in the Basolateral Membrane of Renal Proximal Tubule
Mauerer U, Boulpaep E, Segal A. Regulation of an Inwardly Rectifying ATP-sensitive K+ Channel in the Basolateral Membrane of Renal Proximal Tubule. The Journal Of General Physiology 1998, 111: 161-180. PMID: 9417142, PMCID: PMC1887764, DOI: 10.1085/jgp.111.1.161.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAmbystomaAnimalsBiological TransportCalciumCell MembraneColforsinCyclic AMP-Dependent Protein KinasesEnzyme ActivationEpithelial CellsHydrogen-Ion ConcentrationIon Channel GatingKidney Tubules, ProximalPatch-Clamp TechniquesPhosphorylationPotassium ChannelsProtein Kinase CSodium-Potassium-Exchanging ATPaseConceptsBasolateral membraneProtein kinase AActin cytoskeletonATPase pumpOuter membraneProtein kinaseKinase ACellular metabolismKATP channelsSecond messengerStimulation of transportTransport activityPhysiological importanceMembrane stretchPhorbol esterChannel activityMembrane patchesRegulationIntracellularO patchesFunctional couplingHypotonic swellingCell-attached patchesCell depolarizationRenal proximal tubules
1997
Tubule electrophysiology: from single channels back to the renal epithelium.
Boulpaep E. Tubule electrophysiology: from single channels back to the renal epithelium. Wiener Klinische Wochenschrift 1997, 109: 489-92. PMID: 9261991.Peer-Reviewed Original ResearchConceptsMacroscopic membrane propertiesMicroscopic parametersSingle channel proteinsElectrical propertiesIon flowMacroscopic referenceSingle barrierMembrane patchesRenal epitheliumSingle channelRenal ion transportRenal ion channelsRegulatory domainExperimental errorChannel proteinsPore conductivityIon channelsWhole-cell conductancePatch-clamp technologyBasolateral membraneRenal tubule cellsCell membrane parametersIon selectivityIon transportOpen probability
1996
A novel preparation of dissociated renal proximal tubule cells that maintain epithelial polarity in suspension
Segal A, Boulpaep E, Maunsbach A. A novel preparation of dissociated renal proximal tubule cells that maintain epithelial polarity in suspension. American Journal Of Physiology 1996, 270: c1843-c1863. PMID: 8764170, DOI: 10.1152/ajpcell.1996.270.6.c1843.Peer-Reviewed Original ResearchConceptsEpithelial polarityFunctional polaritySingle cellsMembrane trafficking pathwaysCell surface polarityBasolateral membrane domainsIon channel proteinsBasolateral membraneApical brush borderCell polarityTrafficking pathwaysActin cytoskeletonMembrane domainsProximal tubule cellsMost epithelial cellsChannel proteinsZO-1 proteinTubule cellsJunctional complexesTight junctional complexesPolarized structureSingle cell modelEpithelial cellsRenal proximal tubule cellsZO-1
1995
A Calcium-activated and nucleotide-sensitive nonselective cation channel in M-1 mouse cortical collecting duct cells
Korbmacher C, Volk T, Segal A, Boulpaep E, Frömter E. A Calcium-activated and nucleotide-sensitive nonselective cation channel in M-1 mouse cortical collecting duct cells. The Journal Of Membrane Biology 1995, 146: 29-45. PMID: 7563035, DOI: 10.1007/bf00232678.Peer-Reviewed Original ResearchConceptsSensitive nonselective cation channelNSC channelNonselective cation channelsDuct cellsAmiloride-sensitive sodium absorptionCation channelsChannel activityAdenine nucleotidesVoltage-dependent blockCalcium entryNonselective cation channel activitySodium absorptionCation channel activityCytoplasmic calciumInhibitory effectSpecial physiological conditionsCytoplasmic applicationMiceCGMP-dependent protein kinaseFlufenamic acidSingle-channel current recordingsMajor routeVolume regulationCurrent recordingsCGMP
1987
Electrochemical analysis of renal Na+-glucose cotransport in salamander proximal tubules
Morgunov N, Boulpaep E. Electrochemical analysis of renal Na+-glucose cotransport in salamander proximal tubules. American Journal Of Physiology 1987, 252: f154-f169. PMID: 3812699, DOI: 10.1152/ajprenal.1987.252.1.f154.Peer-Reviewed Original ResearchConceptsLuminal glucoseProximal tubulesFavorable chemical gradientFavorable electrical gradientSodium concentrationSodium-glucose cotransporterIntracellular sodium activityElectrogenic responseSodium activityMM D-glucoseMembrane potentialSalamander proximal tubuleLuminal perfusateOmega X cm2Glucose cotransportGlucose concentrationLuminal membranePhysiological rangeX cm2Luminal membrane potentialGlucoseD-glucoseTubulesLuminal membrane resistancePerfusate
1986
Regulation of single potassium ion channels from apical membrane of rabbit collecting tubule
Hunter M, Lopes A, Boulpaep E, Giebisch G. Regulation of single potassium ion channels from apical membrane of rabbit collecting tubule. American Journal Of Physiology 1986, 251: f725-f733. PMID: 2429562, DOI: 10.1152/ajprenal.1986.251.4.f725.Peer-Reviewed Original ResearchConceptsCortical collecting tubuleCollecting tubuleRabbit cortical collecting tubuleBath calcium concentrationOpen probabilityPatch-clamp techniqueOne-half maximalApical membranePotassium secretionChannel open timeIntracellular calciumPotassium ion channelsChannel open probabilityCalcium concentrationMM calciumChannel activityTubulesIon channelsBath solutionSecretionOpen timeTransmembrane potentialCalciumFunction of calciumApparent Ki
1984
Quantitative ultrastructure and functional correlates in proximal tubule of Ambystoma and Necturus
Maunsbach A, Boulpaep E. Quantitative ultrastructure and functional correlates in proximal tubule of Ambystoma and Necturus. American Journal Of Physiology 1984, 246: f710-f724. PMID: 6720973, DOI: 10.1152/ajprenal.1984.246.5.f710.Peer-Reviewed Original ResearchMeSH KeywordsAmbystomaAnimalsCell MembraneEpitheliumFemaleKidney Tubules, ProximalMitochondriaNecturusConceptsBasal cell membraneCell membraneStructure-function correlationsIntercellular spacesBasal cell surfaceLateral cell membranesBasal extracellular labyrinthMorphometric comparative analysisVolume of cellsInterspecies variabilityCell surfaceAmbystomaQuantitative ultrastructureElaborate organizationBasolateral membraneLateral intercellular spacesSpeciesSalamander Ambystoma tigrinumExtracellular channelsMembraneUltrastructureAmbystoma tigrinumFunctional propertiesProximal tubules
1983
Chloride transport across the basolateral cell membrane of theNecturus proximal tubule: Dependence on bicarbonate and sodium
Guggino W, London R, Boulpaep E, Giebisch G. Chloride transport across the basolateral cell membrane of theNecturus proximal tubule: Dependence on bicarbonate and sodium. The Journal Of Membrane Biology 1983, 71: 227-240. PMID: 6302263, DOI: 10.1007/bf01875464.Peer-Reviewed Original ResearchConceptsBasolateral cell membraneProximal tubule cellsBasolateral solutionNH4Cl exposurePH-sensitive microelectrodesProximal tubulesTubule cellsPerfusion solutionCell membraneIntracellular chlorideTheNecturus proximal tubuleIntracellular pHApical cell membraneNH4Cl treatmentChloride transportChloride activityKidney
1982
Cellular and paracellular resistances of theNecturus proximal tubule
Guggino W, Windhager E, Boulpaep E, Giebisch G. Cellular and paracellular resistances of theNecturus proximal tubule. The Journal Of Membrane Biology 1982, 67: 143-154. PMID: 7097759, DOI: 10.1007/bf01868657.Peer-Reviewed Original ResearchElectrical properties of chloride transport across theNecturus proximal tubule
Guggino W, Boulpaep E, Giebisch G. Electrical properties of chloride transport across theNecturus proximal tubule. The Journal Of Membrane Biology 1982, 65: 185-196. PMID: 7062339, DOI: 10.1007/bf01869962.Peer-Reviewed Original Research
1981
The rheogenic nature of sodium transport in leaky epithelia studied by equivalent circuit analysis.
Sackin H, Boulpaep E. The rheogenic nature of sodium transport in leaky epithelia studied by equivalent circuit analysis. Progress In Clinical And Biological Research 1981, 73: 57-66. PMID: 6275400.Peer-Reviewed Original Research
1979
Effect of amiloride on the apical cell membrane cation channels of a sodium-absorbing, potassium-secreting renal epithelium
O'Neil R, Boulpaep E. Effect of amiloride on the apical cell membrane cation channels of a sodium-absorbing, potassium-secreting renal epithelium. The Journal Of Membrane Biology 1979, 50: 365-387. PMID: 513119, DOI: 10.1007/bf01868898.Peer-Reviewed Original ResearchConceptsLumen-negative transepithelial potential differenceActive Na absorptionApical cell membraneEffects of amilorideEquivalent short-circuit currentTransepithelial potential differenceNa absorptionMaximum inhibitory concentrationAmilorideDiuretic amilorideCation channelsCell membraneNa channelsInhibitory concentrationRenal epitheliumMembrane cation channelsRemoval of NaAbsence of NaTight junctionsPerfusateK permeabilityTubulesLumenModulation of cell membrane area in renal collecting tubules by corticosteroid hormones.
Wade J, O'Neil R, Pryor J, Boulpaep E. Modulation of cell membrane area in renal collecting tubules by corticosteroid hormones. Journal Of Cell Biology 1979, 81: 439-445. PMID: 468913, PMCID: PMC2110313, DOI: 10.1083/jcb.81.2.439.Peer-Reviewed Original Research
1976
Electrical phenomena in the nephron
Boulpaep E. Electrical phenomena in the nephron. Kidney International 1976, 9: 88-102. PMID: 940266, DOI: 10.1038/ki.1976.14.Peer-Reviewed Original ResearchConceptsSingle barrierAvailable experimental dataIon movementElectrical conductanceElectrical phenomenaSingle cell membraneSingle membrane levelElectric currentElectrical propertiesPotential differenceExperimental dataDiffusion barrierDistinctive physical propertiesElectrical characteristicsPhysical propertiesIndividual cell membranesDiffusion boundariesElectrical behaviorPotential gradientMembrane approachConjugate forcesBiological membranesElectrochemical potential gradientTransport processesMicroscopic level
1975
Models for coupling of salt and water transport; Proximal tubular reabsorption in Necturus kidney.
Sackin H, Boulpaep E. Models for coupling of salt and water transport; Proximal tubular reabsorption in Necturus kidney. The Journal Of General Physiology 1975, 66: 671-733. PMID: 1104761, PMCID: PMC2226230, DOI: 10.1085/jgp.66.6.671.Peer-Reviewed Original ResearchConceptsCoupling of saltWater transportDifferent boundary conditionsChemical driving forceSolvent fluxWater fluxTransport parametersBoundary conditionsGradient modelConcentration profilesOsmotic driving forceDriving forceIsotonic transportNet transportDiamondFluxTransportForceWaterFirst modelSecond modelModel
1971
Electrolyte transport in kidney tubule cells
Giebisch G, Boulpaep E, Whittembury G. Electrolyte transport in kidney tubule cells. Philosophical Transactions Of The Royal Society B Biological Sciences 1971, 262: 175-196. PMID: 4399217, DOI: 10.1098/rstb.1971.0088.Peer-Reviewed Original ResearchConceptsTubule cellsTubular levelKidney tubule cellsNet Na transportActive K uptakePotassium secretionNa-K exchange pumpK secretionProximal tubular levelLuminal cell membraneElectrolyte transportNa pumpLines of evidenceTranstubular movementNa transportNet NaCell compartmentExchange pumpSecretionInterstitial fluidCellsLumenPassive leakIonic pumpsUptake