2006
CFTR is required for PKA-regulated ATP sensitivity of Kir1.1 potassium channels in mouse kidney
Lu M, Leng Q, Egan ME, Caplan MJ, Boulpaep EL, Giebisch GH, Hebert SC. CFTR is required for PKA-regulated ATP sensitivity of Kir1.1 potassium channels in mouse kidney. Journal Of Clinical Investigation 2006, 116: 797-807. PMID: 16470247, PMCID: PMC1361349, DOI: 10.1172/jci26961.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAnimalsCurcuminCyclic AMP-Dependent Protein KinasesCystic Fibrosis Transmembrane Conductance RegulatorHydrogen-Ion ConcentrationKidneyMiceMice, Inbred C57BLMice, Inbred CFTRMice, TransgenicMutationOocytesPatch-Clamp TechniquesPotassium Channels, Inwardly RectifyingXenopus laevisConceptsFunctional switchCystic fibrosis transmembrane conductance regulator (CFTR) Cl(-) channelATP sensitivityEffects of CFTRThick ascending limbPotential physiological rolePKA activityRenal K channelsCystic fibrosisPhysiological roleSecretory channelsK channelsRenal tubule epithelial cellsApical membraneCFTRDeltaF508 mutationDistal nephron segmentsCl- channelsK homeostasisTubule epithelial cellsEpithelial cellsTAL cellsPotassium channelsK handlingGlibenclamide sensitivity
1996
A novel preparation of dissociated renal proximal tubule cells that maintain epithelial polarity in suspension
Segal A, Boulpaep E, Maunsbach A. A novel preparation of dissociated renal proximal tubule cells that maintain epithelial polarity in suspension. American Journal Of Physiology 1996, 270: c1843-c1863. PMID: 8764170, DOI: 10.1152/ajpcell.1996.270.6.c1843.Peer-Reviewed Original ResearchConceptsEpithelial polarityFunctional polaritySingle cellsMembrane trafficking pathwaysCell surface polarityBasolateral membrane domainsIon channel proteinsBasolateral membraneApical brush borderCell polarityTrafficking pathwaysActin cytoskeletonMembrane domainsProximal tubule cellsMost epithelial cellsChannel proteinsZO-1 proteinTubule cellsJunctional complexesTight junctional complexesPolarized structureSingle cell modelEpithelial cellsRenal proximal tubule cellsZO-1