2017
Closely-related Borrelia burgdorferi (sensu stricto) strains exhibit similar fitness in single infections and asymmetric competition in multiple infections
Rynkiewicz EC, Brown J, Tufts DM, Huang CI, Kampen H, Bent SJ, Fish D, Diuk-Wasser MA. Closely-related Borrelia burgdorferi (sensu stricto) strains exhibit similar fitness in single infections and asymmetric competition in multiple infections. Parasites & Vectors 2017, 10: 64. PMID: 28166814, PMCID: PMC5292797, DOI: 10.1186/s13071-016-1964-9.Peer-Reviewed Original ResearchConceptsAsymmetric competitive interactionsPatterns of coexistenceBiotic contextCommon disease vectorPathogen communitiesVector-borne pathogensBorrelia burgdorferiLong-term persistencePathogen speciesAsymmetric competitionPathogen dynamicsInfection phenotypesSimilar fitnessTransmission phenotypeDisease vectorsTemperate regionsCompetitive interactionsTransmission advantagePathogen strainsPathogen persistenceBlack-legged tickCompetitive strainsPeromyscus leucopusWhite-footed miceHost
2015
Association between body size and reservoir competence of mammals bearing Borrelia burgdorferi at an endemic site in the northeastern United States
Barbour AG, Bunikis J, Fish D, Hanincová K. Association between body size and reservoir competence of mammals bearing Borrelia burgdorferi at an endemic site in the northeastern United States. Parasites & Vectors 2015, 8: 299. PMID: 26024881, PMCID: PMC4459683, DOI: 10.1186/s13071-015-0903-5.Peer-Reviewed Original ResearchConceptsBody sizeLarge-sized mammalsMedium-sized mammalsDifferent mammalian speciesEastern North AmericaSpecies-specific PCRLyme disease agentSmall mammalsMammalian speciesWhite-footed mouseCommon raccoonSized mammalsMammalsBorrelia burgdorferiPine volesBody massGrey squirrelsVirginia opossumReservoir competenceEastern chipmunksIxodes scapularis ticksDisease agentsStriped skunksSpeciesNymphal Ixodes scapularis ticks
2014
Borrelia burgdorferi Promotes the Establishment of Babesia microti in the Northeastern United States
Dunn JM, Krause PJ, Davis S, Vannier EG, Fitzpatrick MC, Rollend L, Belperron AA, States SL, Stacey A, Bockenstedt LK, Fish D, Diuk-Wasser MA. Borrelia burgdorferi Promotes the Establishment of Babesia microti in the Northeastern United States. PLOS ONE 2014, 9: e115494. PMID: 25545393, PMCID: PMC4278703, DOI: 10.1371/journal.pone.0115494.Peer-Reviewed Original ResearchConceptsB. microtiB. burgdorferiBabesia microtiLyme diseaseRespective causative agentsSpread of babesiosisI. scapularis larvaeInfected micePrimary reservoir hostHigh prevalenceLeucopus miceMiceHuman babesiosisBorrelia burgdorferiLaboratory dataLarval burdenCausative agentMicrotiBurgdorferiCoinfectionReservoir hostsDiseaseR0 modelBasic reproduction numberEnzootic cycleGut Microbiota of the Tick Vector Ixodes scapularis Modulate Colonization of the Lyme Disease Spirochete
Narasimhan S, Rajeevan N, Liu L, Zhao YO, Heisig J, Pan J, Eppler-Epstein R, DePonte K, Fish D, Fikrig E. Gut Microbiota of the Tick Vector Ixodes scapularis Modulate Colonization of the Lyme Disease Spirochete. Cell Host & Microbe 2014, 15: 58-71. PMID: 24439898, PMCID: PMC3905459, DOI: 10.1016/j.chom.2013.12.001.Peer-Reviewed Original ResearchConceptsPeritrophic matrixTranscription factor signal transducerPathogen colonizationLyme disease spirochete Borrelia burgdorferiActivator of transcriptionGut microbiotaArthropod gutsSignal transducerLyme disease spirocheteFunctional linkArthropod vectorsMajor vectorKey glycoproteinsHuman pathogensSpirochete Borrelia burgdorferiGut epitheliumIxodes scapularis ticksColonizationGut epithelial barrierMicrobiotaExpressionGut lumenScapularis ticksBorrelia burgdorferiEpithelial barrier
2013
Quantitative PCR for Detection of Babesia microti in Ixodes scapularis Ticks and in Human Blood
Rollend L, Bent SJ, Krause PJ, Usmani-Brown S, Steeves TK, States SL, Lepore T, Ryan R, Dias F, Mamoun C, Fish D, Diuk-Wasser MA. Quantitative PCR for Detection of Babesia microti in Ixodes scapularis Ticks and in Human Blood. Vector-Borne And Zoonotic Diseases 2013, 13: 784-790. PMID: 24107203, PMCID: PMC3822370, DOI: 10.1089/vbz.2011.0935.Peer-Reviewed Original ResearchConceptsB. microti DNAIxodes scapularis ticksHuman babesiosisScapularis ticksBabesia microtiQuantitative PCRViral-like illnessB. microti infectionAcute infectionBlood transfusionDisparate incidenceMicroti infectionProlonged illnessBlood supplyI. scapularis ticksBlood samplesEpidemiological surveillanceInfected peopleBorrelia miyamotoiB. microtiBorrelia burgdorferiHuman blood samplesI. scapularis nymphsAnaplasma phagocytophilumSpecific quantitative PCR
2012
Transovarial transmission of Borrelia spirochetes by Ixodes scapularis: A summary of the literature and recent observations
Rollend L, Fish D, Childs JE. Transovarial transmission of Borrelia spirochetes by Ixodes scapularis: A summary of the literature and recent observations. Ticks And Tick-borne Diseases 2012, 4: 46-51. PMID: 23238242, DOI: 10.1016/j.ttbdis.2012.06.008.Peer-Reviewed Original ResearchHuman Risk of Infection with Borrelia burgdorferi, the Lyme Disease Agent, in Eastern United States
Diuk-Wasser MA, Hoen AG, Cislo P, Brinkerhoff R, Hamer SA, Rowland M, Cortinas R, Vourc'h G, Melton F, Hickling GJ, Tsao JI, Bunikis J, Barbour AG, Kitron U, Piesman J, Fish D. Human Risk of Infection with Borrelia burgdorferi, the Lyme Disease Agent, in Eastern United States. American Journal Of Tropical Medicine And Hygiene 2012, 86: 320-327. PMID: 22302869, PMCID: PMC3269287, DOI: 10.4269/ajtmh.2012.11-0395.Peer-Reviewed Original ResearchConceptsLyme diseaseHuman riskSpread of infectionHuman infection riskBorrelia burgdorferi sensu strictoRisk factorsBurgdorferi sensu strictoInfection riskTick-borne pathogensLyme disease agentPrevention effortsInfectionBorrelia burgdorferiConfidence intervalsB. burgdorferiInfected nymphsRiskDiseaseRisk focusBurgdorferiUnited StatesDisease agentsHost-seeking nymphsI. scapularis populationsDiagnosisPredicted Outcomes of Vaccinating Wildlife to Reduce Human Risk of Lyme Disease
Tsao K, Fish D, Galvani AP. Predicted Outcomes of Vaccinating Wildlife to Reduce Human Risk of Lyme Disease. Vector-Borne And Zoonotic Diseases 2012, 12: 544-551. PMID: 22251312, DOI: 10.1089/vbz.2011.0731.Peer-Reviewed Original ResearchConceptsMice vaccinationTick biteB. burgdorferi transmissionLyme disease preventionTick blood mealVaccination outcomesHuman riskVaccination effortsDisease preventionLyme diseaseVaccinationInfection prevalenceBorrelia burgdorferiMiceWildlife vaccinationB. burgdorferiCausative agentPredicted outcomeBlood mealVector ticksBiteOutcomesBurgdorferiRiskWildlife reservoirs
2009
Effects of Tick Control by Acaricide Self-Treatment of White-Tailed Deer on Host-Seeking Tick Infection Prevalence and Entomologic Risk for Ixodes scapularis-Borne Pathogens
Hoen A, Rollend L, Papero M, Carroll J, Daniels T, Mather T, Schulze T, Stafford K, Fish D. Effects of Tick Control by Acaricide Self-Treatment of White-Tailed Deer on Host-Seeking Tick Infection Prevalence and Entomologic Risk for Ixodes scapularis-Borne Pathogens. Vector-Borne And Zoonotic Diseases 2009, 9: 431-438. PMID: 19650738, DOI: 10.1089/vbz.2008.0155.Peer-Reviewed Original ResearchConceptsEntomologic riskInfection prevalenceB. burgdorferiPrevalence of infectionFever group spirochetesBacterial coinfectionSelf treatmentTick infection prevalenceLyme diseaseBorrelia miyamotoiB. miyamotoiBacterial agentsPrevalenceBorrelia burgdorferiAnaplasma phagocytophilumA. phagocytophilumAdultsRiskBurgdorferiInfectionDiseaseHost-seeking ticksProportion of ticksAdult ticksTick control
2008
MLST of housekeeping genes captures geographic population structure and suggests a European origin of Borrelia burgdorferi
Margos G, Gatewood A, Aanensen D, Hanincová K, Terekhova D, Vollmer S, Cornet M, Piesman J, Donaghy M, Bormane A, Hurn M, Feil E, Fish D, Casjens S, Wormser G, Schwartz I, Kurtenbach K. MLST of housekeeping genes captures geographic population structure and suggests a European origin of Borrelia burgdorferi. Proceedings Of The National Academy Of Sciences Of The United States Of America 2008, 105: 8730-8735. PMID: 18574151, PMCID: PMC2435589, DOI: 10.1073/pnas.0800323105.Peer-Reviewed Original ResearchConceptsHousekeeping genesIntergenic spacerPopulation structureGeographic population structureChromosomal housekeeping genesMultilocus sequence typing (MLST) schemeB. burgdorferiPhylogenetic signalNorth AmericaEvolutionary relationshipsSequence typing schemeEvolutionary trajectoriesIGS locusSequence dataMLST schemeCultured isolatesGenesBorrelia burgdorferiOuter surface protein CMLST dataDistinct populationsSurface protein CBacterium Borrelia burgdorferiEuropean populationsVector-borne diseases
2006
Epidemic Spread of Lyme Borreliosis, Northeastern United States - Volume 12, Number 4—April 2006 - Emerging Infectious Diseases journal - CDC
Hanincová K, Kurtenbach K, Diuk-Wasser M, Brei B, Fish D. Epidemic Spread of Lyme Borreliosis, Northeastern United States - Volume 12, Number 4—April 2006 - Emerging Infectious Diseases journal - CDC. Emerging Infectious Diseases 2006, 12: 604-611. PMID: 16704808, PMCID: PMC3294694, DOI: 10.3201/eid1204.051016.Peer-Reviewed Original Research
2004
Borrelia burgdorferi Infection in a Natural Population of Peromyscus Leucopus Mice: A Longitudinal Study in an Area Where Lyme Borreliosis Is Highly Endemic
Bunikis J, Tsao J, Luke C, Luna M, Fish D, Barbour A. Borrelia burgdorferi Infection in a Natural Population of Peromyscus Leucopus Mice: A Longitudinal Study in an Area Where Lyme Borreliosis Is Highly Endemic. The Journal Of Infectious Diseases 2004, 189: 1515-1523. PMID: 15073690, DOI: 10.1086/382594.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, BacterialAntigens, BacterialBacterial Outer Membrane ProteinsBlotting, WesternBorrelia burgdorferiConnecticutDisease ReservoirsDNA, BacterialEndemic DiseasesFemaleImmunoenzyme TechniquesIncidenceIxodesLongitudinal StudiesLyme DiseasePeromyscusPolymerase Chain ReactionRodent DiseasesSeasonsSeroepidemiologic StudiesConceptsPeromyscus leucopus miceLeucopus miceTransmission seasonBorrelia burgdorferi infectionPolymerase chain reactionSurface protein CBurgdorferi infectionBlood samplesMice ageEnzyme immunoassayLyme borreliosisMiceSerum samplesProtein CDynamics of infectionBorrelia burgdorferiB. burgdorferiChain reactionLongitudinal studyEnzootic siteInfectionAntibodiesEntire populationBurgdorferiIxodes scapularis
2002
Detection of Attenuated, Noninfectious Spirochetes in Borrelia burgdorferi–Infected Mice after Antibiotic Treatment
Bockenstedt LK, Mao J, Hodzic E, Barthold SW, Fish D. Detection of Attenuated, Noninfectious Spirochetes in Borrelia burgdorferi–Infected Mice after Antibiotic Treatment. The Journal Of Infectious Diseases 2002, 186: 1430-1437. PMID: 12404158, DOI: 10.1086/345284.Peer-Reviewed Original ResearchConceptsPolymerase chain reactionSham-treated miceAntibiotic-treated miceBorrelia burgdorferi infectionReal-time polymerase chain reactionIxodes scapularis ticksAntibiotic therapyNaive miceHistopathologic evidenceAntibiotic treatmentBurgdorferi infectionPersistent infectionSpirochete DNAMiceBorrelia burgdorferiMonthsScapularis ticksChain reactionTherapyXenodiagnosisInfectionSpirochetesLimited durationTreatmentLow levels
2001
Prophylaxis with Single-Dose Doxycycline for the Prevention of Lyme Disease after an Ixodes scapularis Tick Bite
Nadelman R, Nowakowski J, Fish D, Falco R, Freeman K, McKenna D, Welch P, Marcus R, Agüero-Rosenfeld M, Dennis D, Wormser G. Prophylaxis with Single-Dose Doxycycline for the Prevention of Lyme Disease after an Ixodes scapularis Tick Bite. New England Journal Of Medicine 2001, 345: 79-84. PMID: 11450675, DOI: 10.1056/nejm200107123450201.Peer-Reviewed Original ResearchConceptsDose of doxycyclineTick biteLyme diseaseErythema migransSingle-dose doxycyclinePlacebo-controlled trialFrequent adverse effectsMore frequent adverse effectsEfficacy of treatmentSerum antibody testsNymphal ticksAsymptomatic seroconversionPlacebo groupDoxycycline groupBlood culturesDegree of engorgementExtracutaneous signsI. scapularis ticksAntibody testAntimicrobial treatmentDiseaseAdverse effectsDoxycyclineBiteBorrelia burgdorferiEffect of Tick Removal on Transmission of Borrelia burgdorferi and Ehrlichia phagocytophila by Ixodes scapularis Nymphs
Vignes F, Piesman J, Heffernan R, Schulze T, Stafford K, Fish D. Effect of Tick Removal on Transmission of Borrelia burgdorferi and Ehrlichia phagocytophila by Ixodes scapularis Nymphs. The Journal Of Infectious Diseases 2001, 183: 773-778. PMID: 11181154, DOI: 10.1086/318818.Peer-Reviewed Original ResearchConceptsEhrlichia phagocytophilaBorrelia burgdorferiLyme disease endemic areaHuman granulocytic ehrlichiosisDisease-endemic areasScapularis nymphsPolymerase chain reaction analysisSerologic testingField strainsChain reaction analysisSpirochete Borrelia burgdorferiIxodes scapularis nymphsTick removalProbability of infectionHuman infectionsE. phagocytophilaGranulocytic ehrlichiosisInfectionI. scapularis nymphsB. burgdorferiBurgdorferiMiceLyme disease spirochete Borrelia burgdorferiNymphal Ixodes scapularisFed ticks
2000
Acquisition of Coinfection and Simultaneous Transmission of Borrelia burgdorferi and Ehrlichia phagocytophila by Ixodes scapularis Ticks
Levin M, Fish D. Acquisition of Coinfection and Simultaneous Transmission of Borrelia burgdorferi and Ehrlichia phagocytophila by Ixodes scapularis Ticks. Infection And Immunity 2000, 68: 2183-2186. PMID: 10722618, PMCID: PMC97402, DOI: 10.1128/iai.68.4.2183-2186.2000.Peer-Reviewed Original ResearchConceptsHuman granulocytic ehrlichiosisPrior infection statusGranulocytic ehrlichiosisInfected miceLyme diseaseInfection statusI. scapularis nymphsScapularis ticksSecond pathogenSusceptible hostsScapularis nymphsInfected hostIxodes scapularis ticksPrevious infectionI. scapularis ticksEhrlichia phagocytophilaUninfected ticksInfected ticksMiceBorrelia burgdorferiDiseaseEhrlichiosisBorreliaEvidence of interactionNymphal ticks
1999
Disparity in the Natural Cycles of Borrelia burgdorferi and the Agent of Human Granulocytic Ehrlichiosis - Volume 5, Number 2—April 1999 - Emerging Infectious Diseases journal - CDC
Levin M, Vignes F, Fish D. Disparity in the Natural Cycles of Borrelia burgdorferi and the Agent of Human Granulocytic Ehrlichiosis - Volume 5, Number 2—April 1999 - Emerging Infectious Diseases journal - CDC. Emerging Infectious Diseases 1999, 5: 204-208. PMID: 10221871, PMCID: PMC2640706, DOI: 10.3201/eid0502.990203.Peer-Reviewed Original ResearchConceptsHuman granulocytic ehrlichiosisAgent of HGEB. burgdorferi infectionBurgdorferi infectionInfectious Diseases journal - CDCBorrelia burgdorferiWhite-footed miceIxodes scapularis ticksWild white-footed miceDifferent prevalenceGranulocytic ehrlichiosisPrevalenceMiceScapularis ticksInfectionHost-seeking ticksNatural cycleBurgdorferiTicksAgentsEhrlichiosis
1997
Feeding Density Influences Acquisition of Borrelia burgdorferi in Larval Ixodes scapularis (Acari: Ixodidae)
Levin M, Papero M, Fish D. Feeding Density Influences Acquisition of Borrelia burgdorferi in Larval Ixodes scapularis (Acari: Ixodidae). Journal Of Medical Entomology 1997, 34: 569-572. PMID: 9379464, DOI: 10.1093/jmedent/34.5.569.Peer-Reviewed Original ResearchConceptsPrevalence of infectionBorrelia burgdorferi transmissionConsecutive weeksLarval Ixodes scapularisSpirochete prevalenceBorrelia burgdorferiB. burgdorferiLarval I. scapularisInfluences acquisitionPrevalenceBurgdorferiIxodes scapularisWhite-footed mouseIxodes scapularis SayI. scapularisEfficiency of acquisitionI. scapularis populationsInfectionMiceOspA antibodies inhibit the acquisition of Borrelia burgdorferi by Ixodes ticks
de Silva AM, Fish D, Burkot TR, Zhang Y, Fikrig E. OspA antibodies inhibit the acquisition of Borrelia burgdorferi by Ixodes ticks. Infection And Immunity 1997, 65: 3146-3150. PMID: 9234767, PMCID: PMC175444, DOI: 10.1128/iai.65.8.3146-3150.1997.Peer-Reviewed Original ResearchConceptsOspA antibodiesB. burgdorferiPassive antibody transferB. burgdorferi infectionIxodes ticksSurface protein ABurgdorferi-infected miceB. burgdorferi-infected miceLarval infection ratesOuter surface protein AAntibody transferAntibody responseBurgdorferi infectionInfection rateInfected ticksMiceInhibitory effectAntibodiesLarval infectionBorrelia burgdorferiBurgdorferiInfectionLarval ticksReservoir competenceSpirochetes
1996
Duration of Tick Bites in a Lyme Disease-endemic Area
Falco R, Fish D, Piesman J. Duration of Tick Bites in a Lyme Disease-endemic Area. American Journal Of Epidemiology 1996, 143: 187-192. PMID: 8546120, DOI: 10.1093/oxfordjournals.aje.a008728.Peer-Reviewed Original Research