2022
Khorana, Har Gobind
Söll D, RajBhandary U. Khorana, Har Gobind. 2022 DOI: 10.1016/b978-0-12-822563-9.00089-5.Peer-Reviewed Original ResearchGene synthesisDNA genesChemical synthesisChemical biologyGenetic codeMembrane proteinsDNA mutagenesisDNA sequencesSynthesis of DNABacterio-opsinG proteinsSuch profound effectsProton pumpMRNA synthesisGenesDNA sequencingBiological researchSynthesisBiologyPCR amplificationDNA chipChemistryNucleic acidsDNA diagnosticsProtein
1988
Site-directed mutagenesis to fine-tune enzyme specificity
Uemura H, Rogers M, Swanson R, Watson L, Söll D. Site-directed mutagenesis to fine-tune enzyme specificity. Protein Engineering Design And Selection 1988, 2: 293-296. PMID: 3150543, DOI: 10.1093/protein/2.4.293.Peer-Reviewed Original ResearchConceptsOligonucleotide-directed mutagenesisEscherichia coli glutaminyl-tRNA synthetaseGenetic selectionGlutaminyl-tRNA synthetaseAmino acid replacementsSite-directed mutagenesisAcid replacementsEnzyme specificitySingle residueMutagenesisSide chainsRepulsive charge-charge interactionsSpecific recognitionCharge-charge interactionsNucleic acidsMutantsProteinSupFSynthetaseResiduesGlutamineSelection
1986
Proton NMR Studies of RNA’S and Related Enzymes Using Isotope Labels
Redfield A, Choi B, Griffey R, Jarema M, Rosevear P, Hoben P, Swanson R, Soll D. Proton NMR Studies of RNA’S and Related Enzymes Using Isotope Labels. NATO Science Series A: 1986, 99-112. DOI: 10.1007/978-1-4684-5173-3_9.Peer-Reviewed Original ResearchTransfer RNATRNA synthetasesProton NMRAminoacyl-tRNA synthetasesSmall RNA fragmentsDetails of recognitionProton NMR studiesNitrogen-15 labellingSmall proteinsTRNARNA fragmentsSmall enzymeNMR methodologyRelated enzymesNMR studiesRNANMRSmall moleculesLarge moleculesIsotope labelsSynthetasesImportant moleculesEnzymeNucleic acidsMolecules