2019
A versatile flow-based assay for immunocyte-mediated cytotoxicity
Rabinovich PM, Zhang J, Kerr SR, Cheng BH, Komarovskaya M, Bersenev A, Hurwitz ME, Krause DS, Weissman SM, Katz SG. A versatile flow-based assay for immunocyte-mediated cytotoxicity. Journal Of Immunological Methods 2019, 474: 112668. PMID: 31525367, PMCID: PMC6891822, DOI: 10.1016/j.jim.2019.112668.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell Line, TumorCell NucleusCytotoxicity Tests, ImmunologicCytotoxicity, ImmunologicFlow CytometryHigh-Throughput Screening AssaysHumansImmunotherapy, AdoptiveKiller Cells, NaturalLymphocytes, Tumor-InfiltratingMaleMelanomaMice, Inbred C57BLPredictive Value of TestsReceptors, Chimeric AntigenReproducibility of ResultsSkin NeoplasmsTime FactorsT-LymphocytesWorkflowConceptsCell-mediated cytotoxicityTumor-Infiltrating LymphocytesEffector cellsTarget cellsNK-92 cellsChimeric antigen receptorNuclear staining patternInfiltrating lymphocytesT cellsEffector nucleiFlow-based assayImmune systemFlow cytometryStaining patternAntigen receptorDead cellsKilling reactionCytotoxicityCell permeable dyeCellsAssaysCell mixturesNuclear proteinsNovel strategyCell proteinsMKL1-actin pathway restricts chromatin accessibility and prevents mature pluripotency activation
Hu X, Liu ZZ, Chen X, Schulz VP, Kumar A, Hartman AA, Weinstein J, Johnston JF, Rodriguez EC, Eastman AE, Cheng J, Min L, Zhong M, Carroll C, Gallagher PG, Lu J, Schwartz M, King MC, Krause DS, Guo S. MKL1-actin pathway restricts chromatin accessibility and prevents mature pluripotency activation. Nature Communications 2019, 10: 1695. PMID: 30979898, PMCID: PMC6461646, DOI: 10.1038/s41467-019-09636-6.Peer-Reviewed Original ResearchConceptsCell fate reprogrammingChromatin accessibilityActin cytoskeletonSomatic cell reprogrammingPluripotency transcription factorsGlobal chromatin accessibilityGenomic accessibilityCytoskeleton (LINC) complexCell reprogrammingCytoskeletal genesTranscription factorsReprogrammingPluripotencyChromatinCytoskeletonMKL1Unappreciated aspectPathwayNuclear volumeNucleoskeletonSUN2CellsActivationGenesExpression
2012
Induction of megakaryocyte differentiation drives nuclear accumulation and transcriptional function of MKL1 via actin polymerization and RhoA activation
Smith EC, Teixeira AM, Chen RC, Wang L, Gao Y, Hahn KL, Krause DS. Induction of megakaryocyte differentiation drives nuclear accumulation and transcriptional function of MKL1 via actin polymerization and RhoA activation. Blood 2012, 121: 1094-1101. PMID: 23243284, PMCID: PMC3575755, DOI: 10.1182/blood-2012-05-429993.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsCell DifferentiationCell Line, TumorCell NucleusDNA-Binding ProteinsEnzyme ActivationHumansMegakaryocyte Progenitor CellsMegakaryocytesMiceOncogene Proteins, FusionProtein MultimerizationRhoA GTP-Binding ProteinSerum Response FactorTetradecanoylphorbol AcetateThrombopoietinTrans-ActivatorsConceptsMegakaryocyte differentiationActin polymerizationSubcellular localizationSerum response factor (SRF) transcriptional activityRhoA activitySRF target genesComplex cellular responsesFactor transcriptional activityMuscle cell typesCell-type specificHuman erythroleukemia cellsPrimary megakaryocytesTranscriptional regulatorsActin organizationCellular functionsTranscriptional functionSRF activityNuclear localizationTarget genesMegakaryocytic differentiationTranscriptional activityNuclear accumulationErythroleukemia cellsMolecular mechanismsRhoA activation
2007
Rbm15 Modulates Notch-Induced Transcriptional Activation and Affects Myeloid Differentiation
Ma X, Renda MJ, Wang L, Cheng EC, Niu C, Morris SW, S. AS, Krause DS. Rbm15 Modulates Notch-Induced Transcriptional Activation and Affects Myeloid Differentiation. Molecular And Cellular Biology 2007, 27: 3056-3064. PMID: 17283045, PMCID: PMC1899951, DOI: 10.1128/mcb.01339-06.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBasic Helix-Loop-Helix Transcription FactorsCell NucleusCHO CellsCricetinaeCricetulusDNA-Binding ProteinsDrosophila ProteinsGene Expression ProfilingHomeodomain ProteinsImmunoglobulin J Recombination Signal Sequence-Binding ProteinMiceMolecular Sequence DataMyeloid CellsMyelopoiesisNuclear ProteinsPromoter Regions, GeneticProtein BindingProtein Structure, TertiaryProtein TransportReceptors, NotchRNA, MessengerRNA, Small InterferingRNA-Binding ProteinsTranscription Factor HES-1Transcription, GeneticTranscriptional ActivationConceptsN-terminusPromoter activityMyeloid differentiationCell linesCell type-specific mannerMyeloid precursor cell linePrimary murine cellsType-specific mannerDominant negative effectStimulation of NotchHematopoietic cell linesHuman erythroleukemia cellsPrecursor cell lineMurine cell linesHematopoietic stem cellsTranscriptional activationHes1 transcriptionRNA interferenceErythroleukemia cellsFusion proteinHes1 promoter activityMurine cellsFusion partnerHematopoietic cellsRBM15