2012
Generating live cell data using total internal reflection fluorescence microscopy.
Toomre D. Generating live cell data using total internal reflection fluorescence microscopy. Cold Spring Harbor Protocols 2012, 2012: 439-46. PMID: 22474670, DOI: 10.1101/pdb.ip068676.Peer-Reviewed Original ResearchConceptsTotal internal reflection fluorescence microscopyReflection fluorescence microscopyThinner optical planeDynamic cellular processesFluorescence microscopyLive-cell dataCell fluorescent microscopyRefractive indexHigh numerical aperture objective lensNumerical aperture objective lensMembrane traffickingCytoskeleton dynamicsCellular processesCytoskeleton remodelingCell cortexCell signalingTime-lapse moviesDifferent refractive indicesOrganelle levelTransient intermediate statesMolecular manipulationConventional epifluorescence microscopeDeep imagingEvanescent fieldObjective lens
2005
The Abl/Arg substrate ArgBP2/nArgBP2 coordinates the function of multiple regulatory mechanisms converging on the actin cytoskeleton
Cestra G, Toomre D, Chang S, De Camilli P. The Abl/Arg substrate ArgBP2/nArgBP2 coordinates the function of multiple regulatory mechanisms converging on the actin cytoskeleton. Proceedings Of The National Academy Of Sciences Of The United States Of America 2005, 102: 1731-1736. PMID: 15659545, PMCID: PMC547834, DOI: 10.1073/pnas.0409376102.Peer-Reviewed Original ResearchConceptsActin cytoskeletonCOOH-terminal SH3 domainFocal adhesion proteinsBrain-specific splice variantCell adhesion sitesMultiple regulatory mechanismsUbiquitin ligase CblNH2-terminal regionArg tyrosine kinasesActin rufflesWAVE isoformsAdaptor proteinScaffold proteinSH3 domainTyrosine phosphorylationRegulatory proteinsAdhesion proteinsStress fibersRegulatory mechanismsTyrosine kinaseCytoskeletonDomain domainSplice variantsNeuronal synapsesProtein