2012
Kisspeptin Inhibits High-Voltage Activated Ca2+ Channels in GnRH Neurons via Multiple Ca2+ Influx and Release Pathways
Zhang XB, Spergel DJ. Kisspeptin Inhibits High-Voltage Activated Ca2+ Channels in GnRH Neurons via Multiple Ca2+ Influx and Release Pathways. Neuroendocrinology 2012, 96: 68-80. PMID: 22343183, DOI: 10.1159/000335985.Peer-Reviewed Original ResearchConceptsGnRH neuronsGnRH-green fluorescent protein transgenic miceG protein-coupled receptor 54Phospholipase C inhibitor edelfosineWhole-cell patch-clamp recordingsGonadotropin-releasing hormone (GnRH) secretionCanonical transient receptor potential (TRPC) channelsTransient receptor potential channelsRyR agonist caffeinePatch-clamp recordingsChelation of intracellularVGCC activityReceptor 54Kisspeptin-10Hormone secretionPeptide 234KP-10Antagonist caffeineBrain slicesSubsequent fertilityTransgenic miceTRPC channelsNeuronsDependent inhibitionCytoplasmic free
2007
Calcium and Small-Conductance Calcium-Activated Potassium Channels in Gonadotropin-Releasing Hormone Neurons before, during, and after Puberty
Spergel DJ. Calcium and Small-Conductance Calcium-Activated Potassium Channels in Gonadotropin-Releasing Hormone Neurons before, during, and after Puberty. Endocrinology 2007, 148: 2383-2390. PMID: 17289846, PMCID: PMC3315592, DOI: 10.1210/en.2006-1693.Peer-Reviewed Original ResearchConceptsAdult GnRH neuronsSK channel expressionGnRH neuronsGnRH secretionSK currentPubertal increaseGnRH-green fluorescent protein transgenic miceChannel expressionSmall-conductance calcium-activated potassium channelsGonadotropin-releasing hormone (GnRH) neuronsSK channel blocker apaminCalcium-activated potassium channelsCytoplasmic freeChannel blocker apaminExcitatory synaptic inputsWhole-cell techniqueSK channel subtypesHormone neuronsBlocker apaminKisspeptin stimulationChannel blockersSK channelsBrain slicesChannel subtypesSynaptic inputs
1995
L-Type Ca2+ Channels Mediate Joint Modulation by Gamma-Amino-butyric Acid and Glutamate of [Ca2+]i and Neuropeptide Secretion in Immortalized Gonadodropin-Releasing Hormone Neurons
Spergel D, Krsmanovic L, Stojilkovic S, Catt K. L-Type Ca2+ Channels Mediate Joint Modulation by Gamma-Amino-butyric Acid and Glutamate of [Ca2+]i and Neuropeptide Secretion in Immortalized Gonadodropin-Releasing Hormone Neurons. Neuroendocrinology 1995, 61: 499-508. PMID: 7617127, DOI: 10.1159/000126873.Peer-Reviewed Original ResearchConceptsGamma-aminobutyric acidReceptor antagonistReceptor agonistSecretory responseGonadotropin-releasing hormone (GnRH) secretionGlutamate-induced Ca2GnRH secretory responseImmortalized GnRH neuronsGABAB receptor antagonistGABAA receptor antagonistGABAB receptor agonistGABAA receptor agonistL-type Ca2GT1-7 neuronsHormone neuronsGnRH neuronsRole of calciumHormone secretionGamma-AminoNeuropeptide secretionAgonist concentrationsCytoplasmic calciumNeuronsFree mediumGlutamate