2019
Dynamic Network Activation of Hypothalamic MCH Neurons in REM Sleep and Exploratory Behavior
Blanco-Centurion C, Luo S, Spergel DJ, Vidal-Ortiz A, Oprisan S, Van den Pol AN, Liu M, Shiromani PJ. Dynamic Network Activation of Hypothalamic MCH Neurons in REM Sleep and Exploratory Behavior. Journal Of Neuroscience 2019, 39: 4986-4998. PMID: 31036764, PMCID: PMC6670248, DOI: 10.1523/jneurosci.0305-19.2019.Peer-Reviewed Original ResearchConceptsMelanin-concentrating hormoneRapid eye movement (REM) sleepMCH neuronsEye movement sleepREM sleepHypothalamic neuronsMovement sleepHypothalamic melanin-concentrating hormone (MCH) neuronsMelanin-concentrating hormone (MCH) neuronsNeuropeptide melanin-concentrating hormoneHypothalamic MCH neuronsIndividual hypothalamic neuronsMost brain neuronsFunctional activity mapsExploratory behaviorNon-REM sleepCalcium indicator GCaMP6Single neuron activityHormone neuronsNetwork activationBrain neuronsElectrophysiology studySame neuronsQuiet wakingIndicator GCaMP6s
1994
Glutamate Modulates [Ca2+]i and Gonadotropin-Releasing Hormone Secretion in Immortalized Hypothalamic GT1-7 Neurons
Spergel D, Krsmanovic L, Stojilkovic S, Catt K. Glutamate Modulates [Ca2+]i and Gonadotropin-Releasing Hormone Secretion in Immortalized Hypothalamic GT1-7 Neurons. Neuroendocrinology 1994, 59: 309-317. PMID: 7911229, DOI: 10.1159/000126672.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalciumCells, CulturedCycloleucineExcitatory Amino Acid AntagonistsFura-2GlutamatesGlutamic AcidGonadotropin-Releasing HormoneHypothalamusInositol PhosphatesMiceMice, TransgenicNeuronsNeurotoxinsReceptors, AMPAReceptors, GlutamateReceptors, N-Methyl-D-AspartateSpectrometry, FluorescenceConceptsDichlorokynurenic acidGnRH secretionMK-801Immortalized hypothalamic GT1-7 neuronsGonadotropin-Releasing Hormone SecretionOxo-4-isoxazolepropanoic acidAMPA/kainate antagonistGT1-7 cell lineAction of glutamateReceptor subtype agonistsNMDA receptor antagonistConcentration-dependent riseGT1-7 neuronsGT1-7 cellsKainate antagonistSubtype agonistsHormone secretionHypothalamic neuronsReceptor antagonistGlutamate actionSecretory responseNMDA receptorsGlycine siteCyclopentanedicarboxylic acidNeuroendocrine regulation4 Calcium Signaling and Episodic Secretory Responses of GnRH Neurons
Stojilkovic S, Krsmanovic L, Spergel D, Tomic M, Catt K. 4 Calcium Signaling and Episodic Secretory Responses of GnRH Neurons. International Review Of Neurobiology 1994, 20: 68-84. DOI: 10.1016/b978-0-12-185289-4.50010-3.ChaptersBasal GnRH releaseGnRH releaseGnRH neuronsMammalian gonadotropin-releasing hormoneGnRH pulse generatorPulsatile GnRH releaseGonadotropin-releasing hormoneIntracellular calcium concentrationRelease of neurotransmittersGnRH cellsHypothalamic neuronsPreoptic areaSecretory responseMediobasal hypothalamusHypothalamic tissuePortal vesselsPresynaptic terminalsCalcium concentrationNeuronsCalcium signalingPulsatile natureCellsReleaseSynchronized patternHypothalamus