2019
Dynamic Network Activation of Hypothalamic MCH Neurons in REM Sleep and Exploratory Behavior
Blanco-Centurion C, Luo S, Spergel DJ, Vidal-Ortiz A, Oprisan S, Van den Pol AN, Liu M, Shiromani PJ. Dynamic Network Activation of Hypothalamic MCH Neurons in REM Sleep and Exploratory Behavior. Journal Of Neuroscience 2019, 39: 4986-4998. PMID: 31036764, PMCID: PMC6670248, DOI: 10.1523/jneurosci.0305-19.2019.Peer-Reviewed Original ResearchConceptsMelanin-concentrating hormoneRapid eye movement (REM) sleepMCH neuronsEye movement sleepREM sleepHypothalamic neuronsMovement sleepHypothalamic melanin-concentrating hormone (MCH) neuronsMelanin-concentrating hormone (MCH) neuronsNeuropeptide melanin-concentrating hormoneHypothalamic MCH neuronsIndividual hypothalamic neuronsMost brain neuronsFunctional activity mapsExploratory behaviorNon-REM sleepCalcium indicator GCaMP6Single neuron activityHormone neuronsNetwork activationBrain neuronsElectrophysiology studySame neuronsQuiet wakingIndicator GCaMP6s
2018
Deciphering the Contributions of CRH Receptors in the Brain and Pituitary to Stress-Induced Inhibition of the Reproductive Axis
Raftogianni A, Roth LC, García-González D, Bus T, Kühne C, Monyer H, Spergel DJ, Deussing JM, Grinevich V. Deciphering the Contributions of CRH Receptors in the Brain and Pituitary to Stress-Induced Inhibition of the Reproductive Axis. Frontiers In Molecular Neuroscience 2018, 11: 305. PMID: 30214395, PMCID: PMC6125327, DOI: 10.3389/fnmol.2018.00305.Peer-Reviewed Original ResearchCRH receptor type 1Corticotropin-releasing hormoneHPG axisCentral neuronsCRH receptorsPharmacological studiesAcute stressGonadotropin-releasing hormone (GnRH) neuronsMale HPG axisDecrease of plasmaEarlier pharmacological studiesReceptor type 1Stress induced inhibitionHormone neuronsLH releaseGABAergic neuronsCentral infusionPituitary levelMale miceGonadal axisReproductive axisImmunological stressType 2Pituitary culturesBasal conditions
2009
Innervation of Gonadotropin-Releasing Hormone Neurons by Peptidergic Neurons Conveying Circadian or Energy Balance Information in the Mouse
Ward DR, Dear FM, Ward IA, Anderson SI, Spergel DJ, Smith PA, Ebling FJ. Innervation of Gonadotropin-Releasing Hormone Neurons by Peptidergic Neurons Conveying Circadian or Energy Balance Information in the Mouse. PLOS ONE 2009, 4: e5322. PMID: 19390688, PMCID: PMC2669176, DOI: 10.1371/journal.pone.0005322.Peer-Reviewed Original ResearchConceptsGnRH neuronsVasoactive intestinal peptidePeptidergic neuronsGonadotropin-Releasing Hormone NeuronsGnRH secretory systemVIP-IR fibersGonadotropin-releasing hormoneHypothalamic peptide systemsHormone neuronsBasal forebrainIntestinal peptideHypothalamic neuropeptidesPeptidergic fibersReproductive axisTransgenic miceModulatory inputsNeuronsCircadian timing informationPeptidergic signalsMetabolic statusMiceCircadian informationInnervationSignificant proportionPeptide system
2007
Calcium and Small-Conductance Calcium-Activated Potassium Channels in Gonadotropin-Releasing Hormone Neurons before, during, and after Puberty
Spergel DJ. Calcium and Small-Conductance Calcium-Activated Potassium Channels in Gonadotropin-Releasing Hormone Neurons before, during, and after Puberty. Endocrinology 2007, 148: 2383-2390. PMID: 17289846, PMCID: PMC3315592, DOI: 10.1210/en.2006-1693.Peer-Reviewed Original ResearchConceptsAdult GnRH neuronsSK channel expressionGnRH neuronsGnRH secretionSK currentPubertal increaseGnRH-green fluorescent protein transgenic miceChannel expressionSmall-conductance calcium-activated potassium channelsGonadotropin-releasing hormone (GnRH) neuronsSK channel blocker apaminCalcium-activated potassium channelsCytoplasmic freeChannel blocker apaminExcitatory synaptic inputsWhole-cell techniqueSK channel subtypesHormone neuronsBlocker apaminKisspeptin stimulationChannel blockersSK channelsBrain slicesChannel subtypesSynaptic inputs
2004
Neurotrophic effects of BDNF on embryonic gonadotropin‐releasing hormone (GnRH) neurons
Cronin AS, Horan TL, Spergel DJ, Brooks AN, Hastings MH, Ebling FJ. Neurotrophic effects of BDNF on embryonic gonadotropin‐releasing hormone (GnRH) neurons. European Journal Of Neuroscience 2004, 20: 338-344. PMID: 15233743, DOI: 10.1111/j.1460-9568.2004.03490.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBrainBrain-Derived Neurotrophic FactorCell CountCells, CulturedCyclic AMP Response Element-Binding ProteinDose-Response Relationship, DrugDrug InteractionsEmbryo, MammalianGonadotropin-Releasing HormoneGreen Fluorescent ProteinsImmunoenzyme TechniquesImmunohistochemistryLuminescent ProteinsMiceMice, Inbred C57BLMice, TransgenicMitogen-Activated Protein KinasesNeuritesNeuronsOligodeoxyribonucleotides, AntisensePotassium ChlorideReceptor, trkBConceptsBrain-derived neurotrophic factorGnRH cellsNeurotrophic factorMedian eminenceGonadotropin-releasing hormone (GnRH) neuronsNeurite outgrowthGnRH secretory systemGnRH-immunoreactive cellsGonadotropin-releasing hormoneDose-dependent increaseResponse element-binding proteinPhosphorylation of ERKHormone neuronsBDNF treatmentGnRH neuronsNeurotrophic effectsPreoptic areaNeurons migrateReproductive axisElement-binding proteinOlfactory placodeOlfactory tissuePrimary culturesERK pathwayGnRH
2001
Using reporter genes to label selected neuronal populations in transgenic mice for gene promoter, anatomical, and physiological studies
Spergel D, Krüth U, Shimshek D, Sprengel R, Seeburg P. Using reporter genes to label selected neuronal populations in transgenic mice for gene promoter, anatomical, and physiological studies. Progress In Neurobiology 2001, 63: 673-686. PMID: 11165000, DOI: 10.1016/s0301-0082(00)00038-1.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsGreen fluorescent proteinReporter geneGene promoterSubcellular localization signalsCell type-specific expressionPromoter-driven reporter geneCorresponding reporter genesType-specific expressionLocalization signalReporter proteinNeuronal populationsPromoter elementsTransgenic miceFluorescent proteinTransgenic expressionGenesCell typesGonadotropin-releasing hormone (GnRH) neuronsGnRH neuronal networkPhysiological studiesPromoterProteinHormone neuronsBla genesExpression
1999
GABA- and Glutamate-Activated Channels in Green Fluorescent Protein-Tagged Gonadotropin-Releasing Hormone Neurons in Transgenic Mice
Spergel D, Krüth U, Hanley D, Sprengel R, Seeburg P. GABA- and Glutamate-Activated Channels in Green Fluorescent Protein-Tagged Gonadotropin-Releasing Hormone Neurons in Transgenic Mice. Journal Of Neuroscience 1999, 19: 2037-2050. PMID: 10066257, PMCID: PMC6782541, DOI: 10.1523/jneurosci.19-06-02037.1999.Peer-Reviewed Original ResearchConceptsGnRH neuronsPreoptic areaNucleated patchesWhole-cell recordingsApplication of GABANMDA receptor channelsGanglion terminaleHormone neuronsOrganum vasculosumSeptal nucleusDiagonal bandLamina terminalisNeuronal somataAxon terminalsOlfactory bulbMedian eminenceTransgenic miceFiring patternsNeuronsNeuronal dendritesReceptor channelsGABAGreen fluorescent proteinL-glutamateAMPA
1996
Immortalized GnRH Neurons Express Large-Conductance Calcium-Activated Potassium Channels
Spergel D, Catt K, Rojas E. Immortalized GnRH Neurons Express Large-Conductance Calcium-Activated Potassium Channels. Neuroendocrinology 1996, 63: 101-111. PMID: 9053774, DOI: 10.1159/000126946.Peer-Reviewed Original ResearchConceptsGT1-7 cellsGnRH secretory responseGnRH secretionChannel blockersBK channelsSecretory responseGonadotropin-releasing hormone (GnRH) neuronsEffects of charybdotoxinImmortalized GnRH neuronsPerforated-patch recordingsSmall conductance Ca2Patch-clamp recordingsBK channel blockersGamma-aminobutyric acidPatch-clamp techniqueLarge-conductance Ca2Cytoplasmic free Ca2Endoplasmic reticulum Ca2Hormone neuronsGnRH neuronsExcitatory neurotransmitterVoltage-dependent mannerSustained componentFura-2Whole-cell conductance
1995
L-Type Ca2+ Channels Mediate Joint Modulation by Gamma-Amino-butyric Acid and Glutamate of [Ca2+]i and Neuropeptide Secretion in Immortalized Gonadodropin-Releasing Hormone Neurons
Spergel D, Krsmanovic L, Stojilkovic S, Catt K. L-Type Ca2+ Channels Mediate Joint Modulation by Gamma-Amino-butyric Acid and Glutamate of [Ca2+]i and Neuropeptide Secretion in Immortalized Gonadodropin-Releasing Hormone Neurons. Neuroendocrinology 1995, 61: 499-508. PMID: 7617127, DOI: 10.1159/000126873.Peer-Reviewed Original ResearchConceptsGamma-aminobutyric acidReceptor antagonistReceptor agonistSecretory responseGonadotropin-releasing hormone (GnRH) secretionGlutamate-induced Ca2GnRH secretory responseImmortalized GnRH neuronsGABAB receptor antagonistGABAA receptor antagonistGABAB receptor agonistGABAA receptor agonistL-type Ca2GT1-7 neuronsHormone neuronsGnRH neuronsRole of calciumHormone secretionGamma-AminoNeuropeptide secretionAgonist concentrationsCytoplasmic calciumNeuronsFree mediumGlutamate