2021
Neuroanatomy of the GnRH/Kisspeptin System
Spergel D. Neuroanatomy of the GnRH/Kisspeptin System. Masterclass In Neuroendocrinology 2021, 12: 197-229. DOI: 10.1007/978-3-030-86630-3_8.ChaptersKisspeptin neuronsExtra-hypothalamic areasGonadotropin-releasing hormoneGnRH neuronsGnRH secretionHypothalamic GnRHViral tracingTransgenic miceKisspeptin systemNeuronsGnRHGreen fluorescent proteinAnatomical characterizationNeuroanatomyCurrent bodyDendritic zoneTissue clearingRatsAxonsMiceHormoneSecretionBiomarkers
2019
Modulation of Gonadotropin-Releasing Hormone Neuron Activity and Secretion in Mice by Non-peptide Neurotransmitters, Gasotransmitters, and Gliotransmitters
Spergel DJ. Modulation of Gonadotropin-Releasing Hormone Neuron Activity and Secretion in Mice by Non-peptide Neurotransmitters, Gasotransmitters, and Gliotransmitters. Frontiers In Endocrinology 2019, 10: 329. PMID: 31178828, PMCID: PMC6538683, DOI: 10.3389/fendo.2019.00329.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsGnRH neuron activityGnRH secretionNeuron activityNon-peptide neurotransmittersGnRH neuronsGonadotropin-Releasing Hormone Neuron ActivityMouse GnRH neuronsProstaglandin E2Central regulationAdult miceGliotransmittersMouse studiesNitric oxideSecretionNeurotransmittersDirect modulationMiceNeuronsGasotransmitterFurther explorationGonadotropinModulationActivityGABAPostnatal
2018
Neuropeptidergic modulation of GnRH neuronal activity and GnRH secretion controlling reproduction: insights from recent mouse studies
Spergel DJ. Neuropeptidergic modulation of GnRH neuronal activity and GnRH secretion controlling reproduction: insights from recent mouse studies. Cell And Tissue Research 2018, 375: 179-191. PMID: 30078104, DOI: 10.1007/s00441-018-2893-z.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsGnRH neuronsGnRH secretionGnRH neuronal activityGnRH neuronal firingRecent mouse studiesHormone secretionNeuronal activityNeuronal firingNeuropeptidergic modulationMouse studiesCognate receptorsNeuropeptidesNeuronsSecretionMammalian reproductionReproduction-related processesGonadotropinModulationMiceReceptors
2007
Calcium and Small-Conductance Calcium-Activated Potassium Channels in Gonadotropin-Releasing Hormone Neurons before, during, and after Puberty
Spergel DJ. Calcium and Small-Conductance Calcium-Activated Potassium Channels in Gonadotropin-Releasing Hormone Neurons before, during, and after Puberty. Endocrinology 2007, 148: 2383-2390. PMID: 17289846, PMCID: PMC3315592, DOI: 10.1210/en.2006-1693.Peer-Reviewed Original ResearchConceptsAdult GnRH neuronsSK channel expressionGnRH neuronsGnRH secretionSK currentPubertal increaseGnRH-green fluorescent protein transgenic miceChannel expressionSmall-conductance calcium-activated potassium channelsGonadotropin-releasing hormone (GnRH) neuronsSK channel blocker apaminCalcium-activated potassium channelsCytoplasmic freeChannel blocker apaminExcitatory synaptic inputsWhole-cell techniqueSK channel subtypesHormone neuronsBlocker apaminKisspeptin stimulationChannel blockersSK channelsBrain slicesChannel subtypesSynaptic inputs
2005
Impaired reproductive behavior by lack of GluR-B containing AMPA receptors but not of NMDA receptors in hypothalamic and septal neurons.
Shimshek DR, Bus T, Grinevich V, Single FN, Mack V, Sprengel R, Spergel DJ, Seeburg PH. Impaired reproductive behavior by lack of GluR-B containing AMPA receptors but not of NMDA receptors in hypothalamic and septal neurons. Endocrinology 2005, 20: 219-31. PMID: 16099814, DOI: 10.1210/me.2005-0262.Peer-Reviewed Original ResearchConceptsAMPA receptorsNMDA receptorsGnRH secretionMale miceN-methyl-D-aspartate receptorsLimbic system neuronsSerum testosterone levelsIsoxazolepropionic acid (AMPA) receptorsIonotropic glutamate receptorsNormal litter sizeMale sexual behaviorGnRH neuronsSeptal neuronsSystem neuronsOvarian morphologyGlutamate receptorsSeptal areaTestosterone levelsControl littermatesMale littermatesPup retrievalMajor subtypesTestis weightMaternal aggressionAcid receptors
1996
Immortalized GnRH Neurons Express Large-Conductance Calcium-Activated Potassium Channels
Spergel D, Catt K, Rojas E. Immortalized GnRH Neurons Express Large-Conductance Calcium-Activated Potassium Channels. Neuroendocrinology 1996, 63: 101-111. PMID: 9053774, DOI: 10.1159/000126946.Peer-Reviewed Original ResearchConceptsGT1-7 cellsGnRH secretory responseGnRH secretionChannel blockersBK channelsSecretory responseGonadotropin-releasing hormone (GnRH) neuronsEffects of charybdotoxinImmortalized GnRH neuronsPerforated-patch recordingsSmall conductance Ca2Patch-clamp recordingsBK channel blockersGamma-aminobutyric acidPatch-clamp techniqueLarge-conductance Ca2Cytoplasmic free Ca2Endoplasmic reticulum Ca2Hormone neuronsGnRH neuronsExcitatory neurotransmitterVoltage-dependent mannerSustained componentFura-2Whole-cell conductance
1994
Glutamate Modulates [Ca2+]i and Gonadotropin-Releasing Hormone Secretion in Immortalized Hypothalamic GT1-7 Neurons
Spergel D, Krsmanovic L, Stojilkovic S, Catt K. Glutamate Modulates [Ca2+]i and Gonadotropin-Releasing Hormone Secretion in Immortalized Hypothalamic GT1-7 Neurons. Neuroendocrinology 1994, 59: 309-317. PMID: 7911229, DOI: 10.1159/000126672.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalciumCells, CulturedCycloleucineExcitatory Amino Acid AntagonistsFura-2GlutamatesGlutamic AcidGonadotropin-Releasing HormoneHypothalamusInositol PhosphatesMiceMice, TransgenicNeuronsNeurotoxinsReceptors, AMPAReceptors, GlutamateReceptors, N-Methyl-D-AspartateSpectrometry, FluorescenceConceptsDichlorokynurenic acidGnRH secretionMK-801Immortalized hypothalamic GT1-7 neuronsGonadotropin-Releasing Hormone SecretionOxo-4-isoxazolepropanoic acidAMPA/kainate antagonistGT1-7 cell lineAction of glutamateReceptor subtype agonistsNMDA receptor antagonistConcentration-dependent riseGT1-7 neuronsGT1-7 cellsKainate antagonistSubtype agonistsHormone secretionHypothalamic neuronsReceptor antagonistGlutamate actionSecretory responseNMDA receptorsGlycine siteCyclopentanedicarboxylic acidNeuroendocrine regulation