Site-Specific Dephosphorylation of Endothelial Nitric Oxide Synthase by Protein Phosphatase 2A: Evidence for Crosstalk between Phosphorylation Sites †
Greif DM, Kou R, Michel T. Site-Specific Dephosphorylation of Endothelial Nitric Oxide Synthase by Protein Phosphatase 2A: Evidence for Crosstalk between Phosphorylation Sites †. Biochemistry 2002, 41: 15845-15853. PMID: 12501214, DOI: 10.1021/bi026732g.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCattleCell LineCOS CellsEnzyme ActivationEnzyme InhibitorsMarine ToxinsMutagenesis, Site-DirectedNitric Oxide SynthaseNitric Oxide Synthase Type IIIOkadaic AcidOxazolesPhosphatesPhosphoprotein PhosphatasesPhosphorus RadioisotopesPhosphorylationProtein Phosphatase 2SerineSignal TransductionThreonineTransfectionConceptsProtein phosphatase 2ASerine 116Bovine aortic endothelial cellsCOS-7 cellsENOS dephosphorylationENOS mutantPhosphatase 2ASerine 1179Phosphorylation sitesThreonine 497Calcium/calmodulin-dependent enzymesInhibitor of PP2ASite-specific dephosphorylationProtein kinase pathwayWild-type eNOSEnzyme activityNitric oxide-dependent signaling pathwaysCalmodulin-dependent enzymesProtein phosphatasePosttranslational modificationsEpitope tagENOS phosphorylationKinase pathwayEndothelial cellsRecombinant proteinsDephosphorylation of Endothelial Nitric-oxide Synthase by Vascular Endothelial Growth Factor IMPLICATIONS FOR THE VASCULAR RESPONSES TO CYCLOSPORIN A*
Kou R, Greif D, Michel T. Dephosphorylation of Endothelial Nitric-oxide Synthase by Vascular Endothelial Growth Factor IMPLICATIONS FOR THE VASCULAR RESPONSES TO CYCLOSPORIN A*. Journal Of Biological Chemistry 2002, 277: 29669-29673. PMID: 12050171, DOI: 10.1074/jbc.m204519200.Peer-Reviewed Original ResearchConceptsENOS dephosphorylationSerine 116MAP kinase pathway inhibitor U0126Protein phosphatase pathwaysPhosphorylation-deficient mutantDependent protein phosphatasePhosphorylation state-specific antibodiesWild-type enzymeVascular endothelial growth factorProtein kinase C inhibitor calphostinPathway inhibitor U0126Activation of calcineurinProtein phosphataseInhibitor of calcineurinPhosphatase pathwaysENOS agonistsProtein kinaseEndothelial cellsAlanine residuesDephosphorylationDependent enzymesInhibitor U0126Dephosphorylation of eNOSCultured endothelial cellsEnzyme activity