2017
Two transmembrane dimers of the bovine papillomavirus E5 oncoprotein clamp the PDGF β receptor in an active dimeric conformation
Karabadzhak AG, Petti LM, Barrera FN, Edwards APB, Moya-Rodríguez A, Polikanov YS, Freites JA, Tobias DJ, Engelman DM, DiMaio D. Two transmembrane dimers of the bovine papillomavirus E5 oncoprotein clamp the PDGF β receptor in an active dimeric conformation. Proceedings Of The National Academy Of Sciences Of The United States Of America 2017, 114: e7262-e7271. PMID: 28808001, PMCID: PMC5584431, DOI: 10.1073/pnas.1705622114.Peer-Reviewed Original ResearchConceptsTransmembrane domainE5 proteinE5 dimerPlatelet-derived growth factor β receptorGrowth factor β receptorActive dimeric conformationPDGF β-receptorTransmembrane dimerProtein bindsMembrane environmentReceptor dimerizationDimeric conformationAtom molecular dynamics simulationsBiochemical experimentsMouse cellsMolecular mechanismsActive dimerΒ receptorBovine papillomavirusProteinSpecific interactionsMembrane modelingReceptor activationDimerizationComplexes
2016
An Update on Canine, Feline and Bovine Papillomaviruses
da Costa R, Peleteiro M, Pires M, DiMaio D. An Update on Canine, Feline and Bovine Papillomaviruses. Transboundary And Emerging Diseases 2016, 64: 1371-1379. PMID: 27615361, DOI: 10.1111/tbed.12555.Peer-Reviewed Original ResearchConceptsAnimal healthSignificant economic lossesNumber of papillomavirusesCross-species infectionFarm animalsVeterinary practitionersNew viral typesEconomic lossesWild animalsWorldwide importanceFeline papillomavirusesAnimal papillomavirusesPrevalent pathogenDomestic catsPapillomavirus researchCell biologyFascinating modelAnimalsBovine papillomavirusRecent discoveryGreat impactPathogensFelineCanineWide range
2001
Mechanisms of cell transformation by papillomavirus E5 proteins
DiMaio D, Mattoon D. Mechanisms of cell transformation by papillomavirus E5 proteins. Oncogene 2001, 20: 7866-7873. PMID: 11753669, DOI: 10.1038/sj.onc.1204915.Peer-Reviewed Original ResearchConceptsE5 proteinBovine papillomavirus E5 proteinCellular signal transduction pathwaysSignal transduction pathwaysLigand-independent fashionGrowth factor receptor activityReceptor tyrosine kinasesTransforming proteinTransduction pathwaysGrowth factor receptorVacuolar ATPaseReceptor dimerizationTyrosine kinaseCell transformationProteinViral transformationBovine papillomavirusFactor receptorUnique mechanismStable complexesNew insightsReceptor activityPathwayReceptorsKinase
2000
The platelet-derived growth factor ß receptor as a target of the bovine papillomavirus E5 protein
DiMaio D, Lai C, Mattoon D. The platelet-derived growth factor ß receptor as a target of the bovine papillomavirus E5 protein. Cytokine & Growth Factor Reviews 2000, 11: 283-293. PMID: 10959076, DOI: 10.1016/s1359-6101(00)00012-5.Peer-Reviewed Original ResearchConceptsE5 proteinBovine papillomavirus E5 proteinSH2 domain-containing proteinsCellular signal transduction pathwaysDomain-containing proteinsSignal transduction complexSignal transduction pathwaysLigand-independent fashionGrowth factor receptor activitySpecific transmembraneTransduction complexCytoplasmic domainTransmembrane proteinTransduction pathwaysReceptor dimerizationTyrosine residuesAmino acidsProteinViral transformationDirect interactionBovine papillomavirusUnique mechanismStable complexesComplex formationNew insights
1994
The E5 transforming proteins of the papillomaviruses
DiMaio D, Petti L, Hwang E. The E5 transforming proteins of the papillomaviruses. Seminars In Virology 1994, 5: 369-379. DOI: 10.1006/smvy.1994.1041.Peer-Reviewed Original ResearchBPV E5 proteinE5 proteinSimilar cellular targetsΑ-adaptinPDGF β-receptorTransforming proteinCellular proteinsEGF receptorCellular targetsProteinBovine papillomavirusPotential targetCultured fibroblastsΒ receptorImportant mediatorFibroblastsReceptorsSubunitsTargetATPaseInteractsE5Considerable evidenceSpecific interaction between the bovine papillomavirus E5 transforming protein and the beta receptor for platelet-derived growth factor in stably transformed and acutely transfected cells
Petti L, DiMaio D. Specific interaction between the bovine papillomavirus E5 transforming protein and the beta receptor for platelet-derived growth factor in stably transformed and acutely transfected cells. Journal Of Virology 1994, 68: 3582-3592. PMID: 8189497, PMCID: PMC236862, DOI: 10.1128/jvi.68.6.3582-3592.1994.Peer-Reviewed Original ResearchConceptsPDGF beta receptorE5 proteinPlatelet-derived growth factorEGF receptorEpidermal growth factorGrowth factor receptorCOS cellsTumorigenic transformationBovine fibroblastsPDGF receptorHeterologous cell systemFactor receptorNIH 3T3 cellsGrowth factorBovine papillomavirus E5Beta receptorsMembrane proteinsTransient overexpressionRodent fibroblastsCell typesProteinBovine papillomavirusPotential targetSpecific interactionsEpithelial cells
1992
Stable association between the bovine papillomavirus E5 transforming protein and activated platelet-derived growth factor receptor in transformed mouse cells.
Petti L, DiMaio D. Stable association between the bovine papillomavirus E5 transforming protein and activated platelet-derived growth factor receptor in transformed mouse cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 1992, 89: 6736-6740. PMID: 1323117, PMCID: PMC49578, DOI: 10.1073/pnas.89.15.6736.Peer-Reviewed Original ResearchConceptsE5 proteinPlatelet-derived growth factorGrowth factor receptor activationPDGF receptorMouse C127 cellsBovine papillomavirus E5Platelet-derived growth factor receptorShorter proteinTransforming proteinCoimmunoprecipitation analysisGrowth factor receptorReceptor transmitsStable associationC127 cellsTumorigenic transformationMouse cellsProteinBovine papillomavirusFactor receptorDistinct mechanismsStable complexesGrowth factorReceptor activationImportant targetBeta receptors
1989
Genetic Evidence that Acute Morphologic Transformation, Induction of Cellular DNA Synthesis, and Focus Formation Are Mediated by a Single Activity of the Bovine Papillomavirus E5 Protein
Settleman J, Fazeli A, Malicki J, Horwitz B, Dimaio D. Genetic Evidence that Acute Morphologic Transformation, Induction of Cellular DNA Synthesis, and Focus Formation Are Mediated by a Single Activity of the Bovine Papillomavirus E5 Protein. Molecular And Cellular Biology 1989, 9: 5563-5572. DOI: 10.1128/mcb.9.12.5563-5572.1989.Peer-Reviewed Original ResearchInduction of cellular DNA synthesisE5 proteinCellular DNA synthesisBovine papillomavirusC127 cellsBovine papillomavirus E5 proteinDNA synthesisStable cell transformantsMouse C127 cellsMutants expressed normal levelsVirus multiplicity of infectionMissense mutantsDefective phenotypesGenetic evidenceE5 geneE5 activitySerum starvationFocus-forming assayMultiplicity of infectionCell cycleMutation analysisViral genesBiochemical activityMorphological transformationContact inhibition
1987
Genetic and biochemical definition of the bovine papillomavirus E5 transforming protein.
Burkhardt A, DiMaio D, Schlegel R. Genetic and biochemical definition of the bovine papillomavirus E5 transforming protein. The EMBO Journal 1987, 6: 2381-2385. PMID: 2822390, PMCID: PMC553643, DOI: 10.1002/j.1460-2075.1987.tb02515.x.Peer-Reviewed Original ResearchConceptsFirst methionine codonMethionine codonE5 proteinCellular membranesAmino-terminal mutationsFrameshift mutationBovine papillomavirus E5Mutant viral genomesCellular transformationInitiation codonHydrophobic polypeptidesBPV genomeSmall polypeptidesCell transformationCodonViral genomeE5 ORFProteinBovine papillomavirusGenetic alterationsMutationsPolypeptideForm dimersGenomeFrame mutations
1986
Nonsense mutation in open reading frame E2 of bovine papillomavirus DNA
DiMaio D. Nonsense mutation in open reading frame E2 of bovine papillomavirus DNA. Journal Of Virology 1986, 57: 475-480. PMID: 3003380, PMCID: PMC252759, DOI: 10.1128/jvi.57.2.475-480.1986.Peer-Reviewed Original ResearchConceptsMutant viral DNAOpen reading frame E2C127 cellsBovine papillomavirus DNAViral DNAGenetic mapping experimentsNonsense mutationMouse C127 cellsSingle base substitution mutationsHost cell genomeFull-length viral DNAFocus-forming activityBase substitution mutationsWild-type DNAAmber mutationCell genomeOncogenic transformationViral ORFsSubstitution mutationsORF E2Mapping experimentsMutant DNATransformation assaysDNABovine papillomavirus
1984
High-level expression of a cloned HLA heavy chain gene introduced into mouse cells on a bovine papillomavirus vector.
DiMaio D, Corbin V, Sibley E, Maniatis T. High-level expression of a cloned HLA heavy chain gene introduced into mouse cells on a bovine papillomavirus vector. Molecular And Cellular Biology 1984, 4: 340-350. PMID: 6321959, PMCID: PMC368701, DOI: 10.1128/mcb.4.2.340.Peer-Reviewed Original ResearchConceptsHLA antigensBovine papillomavirus vectorCell linesFunction of HLA antigensHuman heavy chainsBovine papillomavirusHeavy chainHuman lymphoblastoid cell linesHeavy chain geneMouse cellsDNA expression vectorsLymphoblastoid cell linesTransformed cellsJY cell lineExtrachromosomal viral DNAHuman histocompatibility antigensSelection in vivoHistocompatibility antigensTransformed cell linesHLA genesChain geneCultured mouse cellsHLAHigh-level expressionAntigenHigh-Level Expression of a Cloned HLA Heavy Chain Gene Introduced into Mouse Cells on a Bovine Papillomavirus Vector
DiMaio D, Corbin V, Sibley E, Maniatis T. High-Level Expression of a Cloned HLA Heavy Chain Gene Introduced into Mouse Cells on a Bovine Papillomavirus Vector. Molecular And Cellular Biology 1984, 4: 340-350. DOI: 10.1128/mcb.4.2.340-350.1984.Peer-Reviewed Original ResearchHLA antigensBovine papillomavirus vectorCell linesFunction of HLA antigensHuman heavy chainsBovine papillomavirusHeavy chainHuman lymphoblastoid cell linesHeavy chain geneMouse cellsDNA expression vectorsLymphoblastoid cell linesTransformed cellsJY cell lineExtrachromosomal viral DNAHuman histocompatibility antigensSelection in vivoHistocompatibility antigensTransformed cell linesHLA genesChain geneCultured mouse cellsHLAHigh-level expressionAntigen