Purification of Endogenous Tagged TRAMP4/5 and Exosome Complexes from Yeast and In Vitro Polyadenylation-Exosome Activation Assays
Zigáčková D, Rájecká V, Vaňáčová Š. Purification of Endogenous Tagged TRAMP4/5 and Exosome Complexes from Yeast and In Vitro Polyadenylation-Exosome Activation Assays. Methods In Molecular Biology 2019, 2062: 237-253. PMID: 31768980, DOI: 10.1007/978-1-4939-9822-7_12.Peer-Reviewed Original ResearchMeSH KeywordsCell NucleusExosome Multienzyme Ribonuclease ComplexExosomesPolyadenylationRNARNA StabilitySaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSerine EndopeptidasesConceptsActivity of exosomesTRAMP complexExosome complexPolyadenylation complexRNA maturationRNA processingProtein complexesProtein domainsAuxiliary proteinsRNA substratesIndividual subunitsHigh processivityYeastParticular mutationAberrant formsActivation assaysRNAExosomesComplexesTRAMPWeak activityProcessivityPurificationSubunitsComplete degradation