2019
Purification of Endogenous Tagged TRAMP4/5 and Exosome Complexes from Yeast and In Vitro Polyadenylation-Exosome Activation Assays
Zigáčková D, Rájecká V, Vaňáčová Š. Purification of Endogenous Tagged TRAMP4/5 and Exosome Complexes from Yeast and In Vitro Polyadenylation-Exosome Activation Assays. Methods In Molecular Biology 2019, 2062: 237-253. PMID: 31768980, DOI: 10.1007/978-1-4939-9822-7_12.Peer-Reviewed Original ResearchConceptsActivity of exosomesTRAMP complexExosome complexPolyadenylation complexRNA maturationRNA processingProtein complexesProtein domainsAuxiliary proteinsRNA substratesIndividual subunitsHigh processivityYeastParticular mutationAberrant formsActivation assaysRNAExosomesComplexesTRAMPWeak activityProcessivityPurificationSubunitsComplete degradation
2018
The role of 3′ end uridylation in RNA metabolism and cellular physiology
Zigáčková D, Vaňáčová Š. The role of 3′ end uridylation in RNA metabolism and cellular physiology. Philosophical Transactions Of The Royal Society B Biological Sciences 2018, 373: 20180171. PMID: 30397107, PMCID: PMC6232591, DOI: 10.1098/rstb.2018.0171.Peer-Reviewed Original ResearchConceptsRNA metabolismRegulation of stabilityCell cycle regulationNumber of pathwaysRNA maturationEukaryotic RNAOrganismal levelCellular physiologyCycle regulationRNA degradationCellular RNADiverse rolesWidespread modificationCell deathUridylationRNAMajority of modificationsCurrent knowledgeLife cycleCritical roleTheme issueRegulationPathwayMetabolismEukaryotes