2021
Single-cell longitudinal analysis of SARS-CoV-2 infection in human airway epithelium identifies target cells, alterations in gene expression, and cell state changes
Ravindra NG, Alfajaro MM, Gasque V, Huston NC, Wan H, Szigeti-Buck K, Yasumoto Y, Greaney AM, Habet V, Chow RD, Chen JS, Wei J, Filler RB, Wang B, Wang G, Niklason LE, Montgomery RR, Eisenbarth SC, Chen S, Williams A, Iwasaki A, Horvath TL, Foxman EF, Pierce RW, Pyle AM, van Dijk D, Wilen CB. Single-cell longitudinal analysis of SARS-CoV-2 infection in human airway epithelium identifies target cells, alterations in gene expression, and cell state changes. PLOS Biology 2021, 19: e3001143. PMID: 33730024, PMCID: PMC8007021, DOI: 10.1371/journal.pbio.3001143.Peer-Reviewed Original ResearchConceptsSARS-CoV-2 infectionSARS-CoV-2Human bronchial epithelial cellsInterferon-stimulated genesCell state changesAcute respiratory syndrome coronavirus 2 infectionSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infectionSyndrome coronavirus 2 infectionCell tropismCoronavirus 2 infectionCoronavirus disease 2019Onset of infectionCell-intrinsic expressionCourse of infectionAir-liquid interface culturesHost-viral interactionsBronchial epithelial cellsSingle-cell RNA sequencingCell typesIL-1Disease 2019Human airwaysDevelopment of therapeuticsDrug AdministrationViral replication
2015
Criteria for Reducing Unnecessary Testing for Herpes Simplex Virus, Varicella-Zoster Virus, Cytomegalovirus, and Enterovirus in Cerebrospinal Fluid Samples from Adults
Wilen CB, Monaco CL, Hoppe-Bauer J, Jackups R, Bucelli RC, Burnham CA. Criteria for Reducing Unnecessary Testing for Herpes Simplex Virus, Varicella-Zoster Virus, Cytomegalovirus, and Enterovirus in Cerebrospinal Fluid Samples from Adults. Journal Of Clinical Microbiology 2015, 53: 887-895. PMID: 25568435, PMCID: PMC4390640, DOI: 10.1128/jcm.03161-14.Peer-Reviewed Original ResearchMeSH KeywordsAdultAgedCerebrospinal FluidCohort StudiesCosts and Cost AnalysisCytomegalovirusEnterovirusHerpesvirus 3, HumanHumansLeukocyte CountLeukocytes, MononuclearMeningitis, AsepticMiddle AgedMolecular Diagnostic TechniquesNucleic Acid Amplification TechniquesRetrospective StudiesSensitivity and SpecificitySimplexvirusConceptsNucleic acid amplification testingVaricella-zoster virusCerebrospinal fluid samplesHSV-1/2Unnecessary testingHerpes simplex virus 1/2Retrospective observational studyCells/Fluid samplesComputerized order setsHerpes simplex virusHealth care costsNAAT assaysImmunocompetent adultsImmunocompetent subjectsValidation cohortAmplification testingObservational studyPositive testCSF samplesEV testingSimplex virusLimited followClinical specificityCare costs