Featured Publications
ESCRT-mediated membrane repair protects tumor-derived cells against T cell attack
Ritter AT, Shtengel G, Xu CS, Weigel A, Hoffman DP, Freeman M, Iyer N, Alivodej N, Ackerman D, Voskoboinik I, Trapani J, Hess HF, Mellman I. ESCRT-mediated membrane repair protects tumor-derived cells against T cell attack. Science 2022, 376: 377-382. PMID: 35446649, DOI: 10.1126/science.abl3855.Peer-Reviewed Original ResearchMeSH KeywordsEndosomal Sorting Complexes Required for TransportGranzymesMembrane GlycoproteinsPerforinPore Forming Cytotoxic ProteinsT-Lymphocytes, CytotoxicConceptsESCRT machineryEndosomal Sorting ComplexCancer-derived cellsESCRT proteinsTarget cellsTumor-derived cellsPlasma membranePerforin poresTransport proteinsMembrane woundsMembrane repairRelease of perforinMachineryCytosolProteinCellsCytolytic attackCytotoxic T lymphocytesESCRTTumor cellsPerforin releaseGranzymesRepairApoptosisCell attack
2019
Spastin tethers lipid droplets to peroxisomes and directs fatty acid trafficking through ESCRT-III
Chang C, Weigel A, Ioannou M, Pasolli H, Xu C, Peale D, Shtengel G, Freeman M, Hess H, Blackstone C, Lippincott-Schwartz J. Spastin tethers lipid droplets to peroxisomes and directs fatty acid trafficking through ESCRT-III. Journal Of Cell Biology 2019, 218: 2583-2599. PMID: 31227594, PMCID: PMC6683741, DOI: 10.1083/jcb.201902061.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphatasesAmino Acid MotifsATP Binding Cassette Transporter, Subfamily D, Member 1Biological TransportEndosomal Sorting Complexes Required for TransportFatty AcidsHeLa CellsHumansHydrolysisLauric AcidsLipid DropletsModels, BiologicalMutant ProteinsOncogene ProteinsPeroxisomesSpastinConceptsFA traffickingLipid dropletsESCRT-III componentsNeutral lipid storage organellesLipid storage organellesFatty acid traffickingAAA-ATPaseESCRT-IIIDual roleMIT domainFatty acidsStorage organellesContact sitesPathogenesis of diseaseFA metabolismTraffickingPeroxisomesSpastinIST1OrganellesCHMP1BInterrelated mechanismsLipid peroxidationContact formationATPase
2018
The ESCRT-III Protein CHMP1A Mediates Secretion of Sonic Hedgehog on a Distinctive Subtype of Extracellular Vesicles
Coulter ME, Dorobantu CM, Lodewijk GA, Delalande F, Cianferani S, Ganesh VS, Smith RS, Lim ET, Xu CS, Pang S, Wong ET, Lidov HGW, Calicchio ML, Yang E, Gonzalez DM, Schlaeger TM, Mochida GH, Hess H, Lee WA, Lehtinen MK, Kirchhausen T, Haussler D, Jacobs FMJ, Gaudin R, Walsh CA. The ESCRT-III Protein CHMP1A Mediates Secretion of Sonic Hedgehog on a Distinctive Subtype of Extracellular Vesicles. Cell Reports 2018, 24: 973-986.e8. PMID: 30044992, PMCID: PMC6178983, DOI: 10.1016/j.celrep.2018.06.100.Peer-Reviewed Original ResearchMeSH KeywordsAdultAnimalsBrainChoroid PlexusEndosomal Sorting Complexes Required for TransportExtracellular VesiclesHedgehog ProteinsHumansInfant, NewbornMiceNIH 3T3 CellsVesicular Transport ProteinsConceptsExtracellular vesiclesMultivesicular bodiesIntraluminal vesicle formationESCRT proteinsHedgehog secretionProgenitor maintenanceMolecular mechanistic insightsComplex proteinsCell communicationEV subtypesVesicle formationChmp1AAutosomal recessive microcephalyHuman cerebral organoidsSonic hedgehogProgenitor proliferationFunction mutationsImpairs secretionAdult functionMechanistic insightsCerebral organoidsNull micePontocerebellar hypoplasiaShhProtein