Chloroplast Sec14-like 1 (CPSFL1) is essential for normal chloroplast development and affects carotenoid accumulation in Chlamydomonas
García-Cerdán JG, Schmid EM, Takeuchi T, McRae I, McDonald KL, Yordduangjun N, Hassan AM, Grob P, Xu CS, Hess HF, Fletcher DA, Nogales E, Niyogi KK. Chloroplast Sec14-like 1 (CPSFL1) is essential for normal chloroplast development and affects carotenoid accumulation in Chlamydomonas. Proceedings Of The National Academy Of Sciences Of The United States Of America 2020, 117: 12452-12463. PMID: 32404426, PMCID: PMC7275715, DOI: 10.1073/pnas.1916948117.Peer-Reviewed Original ResearchConceptsChloroplast developmentCarotenoid accumulationNormal chloroplast developmentBiosynthesis of carotenoidsEndosymbiotic originInsertional mutantsDomain proteinsIsoprenoid precursorsOxygenic photosynthesisChloroplast structureSuch proteinsAuxiliary proteinsCarotenoid transportPhytoene synthesisLow carotenoid contentMutantsCommitted precursorsProteinChlamydomonasEssential roleImpaired biosynthesisCarotenoid contentPhotosynthesisBiosynthesisIon beam scanning electron microscopy