2024
Immune modulation in transplant medicine: a comprehensive review of cell therapy applications and future directions
Knoedler L, Dean J, Diatta F, Thompson N, Knoedler S, Rhys R, Sherwani K, Ettl T, Mayer S, Falkner F, Kilian K, Panayi A, Iske J, Safi A, Tullius S, Haykal S, Pomahac B, Kauke-Navarro M. Immune modulation in transplant medicine: a comprehensive review of cell therapy applications and future directions. Frontiers In Immunology 2024, 15: 1372862. PMID: 38650942, PMCID: PMC11033354, DOI: 10.3389/fimmu.2024.1372862.Peer-Reviewed Original ResearchConceptsRegulatory myeloid cellsSolid organ transplantationVascularized composite allotransplantationMesenchymal stromal cellsT cellsTransplant patientsCellular therapyChimeric antigen receptor T cellsTransplant surgerySide effectsIncreased risk of malignancyProgression to chronic rejectionAcute rejection ratesPerioperative treatment strategiesRegulatory T cellsPromote immune toleranceRisk of malignancySystemic side effectsRelated side effectsCell typesImprove transplant survivalCAR-TCell therapy applicationsImmunosuppressive regimensChronic rejection
2022
mTORC1 Inhibition Protects Human Regulatory T Cells From Granzyme-B-Induced Apoptosis
Eskandari SK, Allos H, Al Dulaijan B, Melhem G, Sulkaj I, Alhaddad JB, Saad AJ, Deban C, Chu P, Choi JY, Kollar B, Pomahac B, Riella LV, Berger SP, Sanders JSF, Lieberman J, Li L, Azzi JR. mTORC1 Inhibition Protects Human Regulatory T Cells From Granzyme-B-Induced Apoptosis. Frontiers In Immunology 2022, 13: 899975. PMID: 35757726, PMCID: PMC9229986, DOI: 10.3389/fimmu.2022.899975.Peer-Reviewed Original ResearchConceptsRegulatory T cellsT cellsHuman regulatory T cellsHost THumanized mouse modelPro-apoptotic pathwaysSkin allotransplantationClinical efficacyImmunosuppressive potencyIntracytoplasmic expressionTransplant fieldMouse modelCellular therapyHigh expressionTarget cellsRapamycin complex 1Lysosomal granulesFunctional effectsMechanistic targetPatientsApoptosisC-JunGranzymesDrug targetsS6K
2020
Local immunosuppression in vascularized composite allotransplantation (VCA): A systematic review
Safi AF, Kauke M, Nelms L, Palmer WJ, Tchiloemba B, Kollar B, Haug V, Pomahač B. Local immunosuppression in vascularized composite allotransplantation (VCA): A systematic review. Journal Of Plastic Reconstructive & Aesthetic Surgery 2020, 74: 327-335. PMID: 33229219, DOI: 10.1016/j.bjps.2020.10.003.Peer-Reviewed Original ResearchConceptsLocal immunosuppressionSystematic reviewAllograft survivalComposite allotransplantationAllograft survival dataCell-based therapy strategiesImproved allograft survivalMain immunosuppressive agentMeta-Analyses (PRISMA) guidelinesPreclinical animal studiesPreferred Reporting ItemsMalignant side effectsImmunosuppressive agentsPreclinical dataSignificant prolongationRat modelHuman trialsExclusion criteriaImmunosuppressionSide effectsAnimal studiesReporting ItemsPubMed databaseSystemic toxicityTherapy strategies
2019
Twenty-Four-Hour Ex Vivo Perfusion with Acellular Solution Enables Successful Replantation of Porcine Forelimbs.
Krezdorn N, Macleod F, Tasigiorgos S, Turk M D M, Wo L, Kiwanuka B A H, Lopdrup B I D R, Kollar B, Edelman E, Pomahac B. Twenty-Four-Hour Ex Vivo Perfusion with Acellular Solution Enables Successful Replantation of Porcine Forelimbs. Plastic & Reconstructive Surgery 2019, 144: 608e-618e. PMID: 31568296, DOI: 10.1097/prs.0000000000006084.Peer-Reviewed Original Research
2018
The Collagenase of the Bacterium Clostridium histolyticum in the Treatment of Irradiation-Induced Capsular Contracture
Diehm Y, Hirche C, Berger M, Heil J, Golatta M, Kotsougiani D, Pomahac B, Kneser U, Fischer S. The Collagenase of the Bacterium Clostridium histolyticum in the Treatment of Irradiation-Induced Capsular Contracture. Aesthetic Plastic Surgery 2018, 43: 836-844. PMID: 30456640, DOI: 10.1007/s00266-018-1267-y.Peer-Reviewed Original ResearchConceptsBacterium Clostridium histolyticumCapsular fibrosisHematoma formationTreatment optionsImplant pocketClostridium histolyticumSilicone implantsMiniature silicone implantsNovel treatment optionsPotential treatment optionInjection of collagenaseVessel wall architectureMethodsThirty-six ratsVessel wall thicknessCollagenase injectionSignificant complicationsBreast reconstructionCapsular contractureClinical safetyBreast cancerDay 1Application of collagenaseDay 120Implant sitesThin capsuleReduced Hypoxia-Related Genes in Porcine Limbs in Ex Vivo Hypothermic Perfusion Versus Cold Storage
Krezdorn N, Sakthivel D, Turk M, Aycart M, Tasigiorgos S, Bueno E, Sinha I, Pomahac B. Reduced Hypoxia-Related Genes in Porcine Limbs in Ex Vivo Hypothermic Perfusion Versus Cold Storage. Journal Of Surgical Research 2018, 232: 137-145. PMID: 30463709, DOI: 10.1016/j.jss.2018.05.067.Peer-Reviewed Original ResearchConceptsStatic cold storagePerfusion groupIschemia-reperfusion injuryPCR gene arrayPorcine limbsMuscle fiber disruptionIschemic injuryPerfadex solutionPerfused limbMachine perfusionHypothermic perfusionHistological changesPreservation modalityMuscle biopsyLimb replantationPerfusionICE groupLess activationPorcine forelimbDownregulation of genesMolecular changesLimbFiber disruptionExpression levelsReplantationExtracorporeal Perfusion in Vascularized Composite Allotransplantation
Kueckelhaus M, Puscz F, Dermietzel A, Dadras M, Fischer S, Krezdorn N, Pomahac B, Hirsch T. Extracorporeal Perfusion in Vascularized Composite Allotransplantation. Annals Of Plastic Surgery 2018, 80: 669-678. PMID: 29746324, DOI: 10.1097/sap.0000000000001477.Peer-Reviewed Original ResearchConceptsVascularized Composite AllotransplantationSolid organ transplantationIschemia timeMachine perfusionOrgan transplantationReconstructive surgeryComposite allotransplantationClinical solid organ transplantationMachine perfusion systemImmunosuppressive treatmentGraft immunogenicityBroad clinical applicationLifelong monitoringTherapeutic measuresPerfusion strategyClinical successSevere injuriesPerfusion solutionPreclinical trialsClinical settingPlastic surgeryPerfusionImmunogenicityPerfusion systemClinical application
2017
Acellular Hypothermic Extracorporeal Perfusion Extends Allowable Ischemia Time in a Porcine Whole Limb Replantation Model
Kueckelhaus M, Dermietzel A, Alhefzi M, Aycart M, Fischer S, Krezdorn N, Wo L, Maarouf O, Riella L, Abdi R, Bueno E, Pomahac B. Acellular Hypothermic Extracorporeal Perfusion Extends Allowable Ischemia Time in a Porcine Whole Limb Replantation Model. Plastic & Reconstructive Surgery 2017, 139: 922e-932e. PMID: 28350667, DOI: 10.1097/prs.0000000000003208.Peer-Reviewed Original Research
2016
Long-Term Effects of the Collagenase of the Bacterium Clostridium histolyticum for the Treatment of Capsular Fibrosis After Silicone Implants
Fischer S, Diehm Y, Henzler T, Berger M, Kolbenschlag J, Latz A, Bueno E, Hirche C, Kneser U, Pomahac B. Long-Term Effects of the Collagenase of the Bacterium Clostridium histolyticum for the Treatment of Capsular Fibrosis After Silicone Implants. Aesthetic Plastic Surgery 2016, 41: 211-220. PMID: 28008464, DOI: 10.1007/s00266-016-0724-8.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiopsy, NeedleBreast ImplantationBreast ImplantsClostridium histolyticumDisease Models, AnimalFemaleFibrosisHumansImmunohistochemistryImplant Capsular ContractureInjections, IntralesionalMagnetic Resonance ImagingMicrobial CollagenasePregnancyRandom AllocationRatsRats, Inbred LewReal-Time Polymerase Chain ReactionReference ValuesSilicone GelsTreatment OutcomeUltrasonography, DopplerConceptsMagnetic resonance imagingBacterium Clostridium histolyticumSilicone implantsCapsular fibrosisCollagenase injectionTreatment groupsSkin perforationFrequent long-term complicationMethodsForty-eight ratsMiniature silicone implantsLong-term complicationsClostridium histolyticumViable treatment optionLevel of evidenceHigh-resolution ultrasoundCapsule thicknessInjection of collagenaseExpression levelsDays post injectionLevel AssignedThis journalLong-term effectivenessBackgroundCapsular contractureAnti-fibrotic genesTreatment optionsImplant pocketA Mobile Extracorporeal Extremity Salvage System for Replantation and Transplantation
Kueckelhaus M, Fischer S, Sisk G, Kiwanuka H, Bueno E, Dermietzel A, Alhefzi M, Aycart M, Diehm Y, Pomahac B. A Mobile Extracorporeal Extremity Salvage System for Replantation and Transplantation. Annals Of Plastic Surgery 2016, 76: 355-360. PMID: 26808757, DOI: 10.1097/sap.0000000000000681.Peer-Reviewed Original ResearchConceptsMuscle damageLimb lossPerfusion deviceDeceased organ donorsIschemic muscle damageBilateral hind limbsPreferred treatmentBilateral limbsMuscle biopsyOrgan donorsTraumatic amputationHealthy limbPerfusion solutionReplantationArterial systemTransplantationHypoxic cellsPerfusate compositionHind limbPorcine limbsLimbSignificant differencesYorkshire pigsSalvage systemSimilar changes
2015
The Collagenase of the Bacterium Clostridium histolyticum for the Treatment of Capsular Fibrosis after Silicone Implants
Fischer S, Hirsch T, Diehm Y, Kiefer J, Bueno E, Kueckelhaus M, Kremer T, Hirche C, Kneser U, Pomahac B. The Collagenase of the Bacterium Clostridium histolyticum for the Treatment of Capsular Fibrosis after Silicone Implants. Plastic & Reconstructive Surgery 2015, 136: 981-989. PMID: 26171751, DOI: 10.1097/prs.0000000000001698.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiopsy, NeedleBreast ImplantsClostridium histolyticumDisease Models, AnimalFemaleFibrosisImmunohistochemistryImplant Capsular ContractureMagnetic Resonance ImagingMicrobial CollagenaseRandom AllocationRatsReal-Time Polymerase Chain ReactionReference ValuesSilicone GelsTreatment OutcomeConceptsBacterium Clostridium histolyticumSilicone implantsSkin perforationFibrotic capsuleMiniature silicone implantsClostridium histolyticumMagnetic resonance imagingInflammatory markersDosage-dependent decreasePolymerase chain reaction analysisCapsular fibrosisSafe clinical applicationCapsular contractureDupuytren's contractureMechanical irritationChain reaction analysisControl groupDrug AdministrationResonance imagingHistologic measurementsU.S. FoodThin capsuleVivo studiesSkin digestionCollagen densityVascularized composite allotransplantation: current standards and novel approaches to prevent acute rejection and chronic allograft deterioration
Kueckelhaus M, Fischer S, Seyda M, Bueno E, Aycart M, Alhefzi M, ElKhal A, Pomahac B, Tullius S. Vascularized composite allotransplantation: current standards and novel approaches to prevent acute rejection and chronic allograft deterioration. Transplant International 2015, 29: 655-662. PMID: 26265179, PMCID: PMC4785085, DOI: 10.1111/tri.12652.Peer-Reviewed Original ResearchConceptsAcute rejectionImproved long-term graft outcomesLong-term graft outcomeChronic allograft deteriorationCytomegalovirus infection statusGraft preservation techniquesUpper extremity transplantationUpper extremity transplantsAntibody-mediated rejectionGood midterm resultsSpecific treatment strategiesAllograft deteriorationChronic rejectionClinical VCAVCA graftsGraft outcomeImmunosuppressive strategiesLocal immunosuppressionMidterm resultsExtremity transplantationCell-based therapiesHLA matchingTolerance inductionTreatment strategiesUS CentersSilicone Implants with Smooth Surfaces Induce Thinner but Denser Fibrotic Capsules Compared to Those with Textured Surfaces in a Rodent Model
Fischer S, Hirche C, Reichenberger M, Kiefer J, Diehm Y, Mukundan S, Alhefzi M, Bueno E, Kneser U, Pomahac B. Silicone Implants with Smooth Surfaces Induce Thinner but Denser Fibrotic Capsules Compared to Those with Textured Surfaces in a Rodent Model. PLOS ONE 2015, 10: e0132131. PMID: 26151888, PMCID: PMC4495016, DOI: 10.1371/journal.pone.0132131.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, CDAntigens, Differentiation, MyelomonocyticBreast DiseasesBreast ImplantationBreast ImplantsCollagen Type IDisease Models, AnimalFemaleFibrosisGene ExpressionHumansImplant Capsular ContractureMagnetic Resonance ImagingMammaplastyRadiographyRats, Inbred LewReverse Transcriptase Polymerase Chain ReactionSilicone GelsSurface PropertiesUltrasonographySurgical Anatomy of the Whole Facial Nerve for Enabling Craniofacial and Regenerative Medicine Translational Research in Swine
Aycart M, Alhefzi M, Bueno E, Pomahac B. Surgical Anatomy of the Whole Facial Nerve for Enabling Craniofacial and Regenerative Medicine Translational Research in Swine. Journal Of Reconstructive Microsurgery 2015, 31: 547-550. PMID: 26115545, DOI: 10.1055/s-0035-1555113.Peer-Reviewed Original Research
2014
Hand transplants and the mandate for tolerance
Koulmanda M, Pomahac B, Fan Z, Murphy G, Strom T. Hand transplants and the mandate for tolerance. Current Opinion In Organ Transplantation 2014, Publish Ahead of Print: &na;. PMID: 25373855, PMCID: PMC4352932, DOI: 10.1097/mot.0000000000000138.Peer-Reviewed Original ResearchConceptsHand transplantationIL-2Hand transplantLow-dose IL-2IL-2-based therapyBalance of immunityDrug-free toleranceRegulatory T cellsUse of immunosuppressionRecent clinical trialsLife-long immunosuppressionInduction of toleranceNonhuman primate modelShort-term treatmentDrug minimizationPosttransplant periodInflammatory processClinical trialsPrimate modelT cellsPreclinical demonstrationComposite allograftsAdverse inflammationTransplantationTransplantAcute rejection in vascularized composite allotransplantation
Fischer S, Lian C, Kueckelhaus M, Strom T, Edelman E, Clark R, Murphy G, Chandraker A, Riella L, Tullius S, Pomahac B. Acute rejection in vascularized composite allotransplantation. Current Opinion In Organ Transplantation 2014, 19: 531-544. PMID: 25333831, DOI: 10.1097/mot.0000000000000140.Peer-Reviewed Original ResearchConceptsAcute rejection episodesAcute rejectionRejection episodesComposite allotransplantationDepth histological analysisHigh-dose steroidsLong-term safetyField of transplantationBetter diagnostic toolsFace transplant recipientsMaintenance immunosuppressionSteroid withdrawalTransplant recipientsCommon complicationImmunologic eventsAppropriate therapyPatient numbersTimely institutionRestorative surgeryDose reductionEarly diagnosisMost episodesNovel biomarkersHigh immunogenicityPatient care
2013
Lessons Learned from Simultaneous Face and Bilateral Hand Allotransplantation
Carty M, Hivelin M, Dumontier C, Talbot S, Benjoar M, Pribaz J, Lantieri L, Pomahac B. Lessons Learned from Simultaneous Face and Bilateral Hand Allotransplantation. Plastic & Reconstructive Surgery 2013, 132: 423-432. PMID: 23584623, DOI: 10.1097/prs.0b013e318295883d.Peer-Reviewed Original Research
2012
Epidermal Regeneration by Micrograft Transplantation with Immediate 100-Fold Expansion
Hackl F, Bergmann J, Granter S, Koyama T, Kiwanuka E, Zuhaili B, Pomahac B, Caterson E, Junker J, Eriksson E. Epidermal Regeneration by Micrograft Transplantation with Immediate 100-Fold Expansion. Plastic & Reconstructive Surgery 2012, 129: 443e-452e. PMID: 22373992, DOI: 10.1097/prs.0b013e318241289c.Peer-Reviewed Original ResearchConceptsSplit-thickness skin graftSkin graftsDermal sideEarly clinical resultsCorresponding time pointsFull-thickness woundsMicrograft transplantationDiabetic pigsClinical resultsMajor burnsCurrent treatmentSkin micrograftsComplete epithelializationDiabetic woundsWound chambersPercent reepithelializationGraftChronic woundsHealthy pigsTime pointsWound repairWoundsTransplantationMicrograftsEpidermal regeneration
2010
Meshed Skin Grafts Placed Upside Down Can Take If Desiccation Is Prevented
Zuhaili B, Aflaki P, Koyama T, Fossum M, Reish R, Schmidt B, Pomahac B, Eriksson E. Meshed Skin Grafts Placed Upside Down Can Take If Desiccation Is Prevented. Plastic & Reconstructive Surgery 2010, 125: 855-865. PMID: 20195112, PMCID: PMC2848958, DOI: 10.1097/prs.0b013e3181ccdc42.Peer-Reviewed Original ResearchConceptsSkin graftsMeshed graftReepithelialization rateSplit-thickness skin graftMeshed skin graftAutologous split-thickness skin graftWound environmentFull-thickness woundsHistologic specimensAccelerated reepithelializationDay 4GraftHigh contraction rateWound contractionSheet graftsWound healingWoundsYorkshire pigsSignificant differencesSurvivalHealingRegular graftsReepithelializationContraction rateCurrent study
2009
Use of a non–cross-linked porcine dermal scaffold in abdominal wall reconstruction
Pomahac B, Aflaki P. Use of a non–cross-linked porcine dermal scaffold in abdominal wall reconstruction. The American Journal Of Surgery 2009, 199: 22-27. PMID: 19427629, DOI: 10.1016/j.amjsurg.2008.12.033.Peer-Reviewed Original ResearchMeSH KeywordsAbdominal WallAdultAgedAged, 80 and overAnimalsBiocompatible MaterialsCohort StudiesCollagenFemaleFollow-Up StudiesHernia, AbdominalHernia, VentralHumansMaleMiddle AgedPlastic Surgery ProceduresRetrospective StudiesSeverity of Illness IndexSurgical Wound DehiscenceSuture TechniquesSwineTensile StrengthTreatment OutcomeWound HealingYoung AdultConceptsAbdominal wall reconstructionSuperficial wound dehiscenceAbdominal wallComplex abdominal wallWall reconstructionWound dehiscencePorcine dermalProsthetic meshRetrospective chart reviewIntra-abdominal catastropheSurgical site infectionLarge incisional herniasGiant ventral herniasComplicated abdominal wallMean followMultiorgan failureAbdominal traumaEmergency surgeryOpen abdomenChart reviewSite infectionAbdominal closureFistula formationWound infectionIncisional hernia