1997
Reconstitution of calcium-regulated parathyroid hormone secretion from streptolysin-O-permeabilized parathyroid cells by guanosine 5'-O-(thio)triphosphate.
Matovcik L, Rhee S, Schaefer J, Kinder B. Reconstitution of calcium-regulated parathyroid hormone secretion from streptolysin-O-permeabilized parathyroid cells by guanosine 5'-O-(thio)triphosphate. Endocrinology 1997, 138: 1170-9. PMID: 9048624, DOI: 10.1210/endo.138.3.4971.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBacterial ProteinsCalciumCalcium-Calmodulin-Dependent Protein Kinase Type 2Calcium-Calmodulin-Dependent Protein KinasesCattleCell Membrane PermeabilityCyclic AMPCytosolEnergy MetabolismGuanine NucleotidesGuanosine 5'-O-(3-Thiotriphosphate)Osmolar ConcentrationParathyroid GlandsParathyroid HormoneParotid GlandStreptolysinsTime FactorsConceptsPTH secretionParathyroid cellsIntracellular Ca2Hormone secretionParathyroid hormone secretionGuanosine 5'Dose-dependent mannerCalcium/calmodulin-dependent protein kinase IIPTH releaseCalmodulin-dependent protein kinase IIExtracellular Ca2Physiological intracellular Ca2Protein kinase IISecretionAmbient Ca2Low Ca2Ca2GTPγSCellsInverse relationshipCytosolic cofactorsKinase IIAddition of GTPγSAbsence of GTPγSDependent regulation
1994
Antibodies to an epitope on synapsin I detect a protein associated with the endocytic compartment in non-neuronal cells.
Matovcik LM, Karapetian O, Czernik AJ, Marino CR, Kinder BK, Gorelick FS. Antibodies to an epitope on synapsin I detect a protein associated with the endocytic compartment in non-neuronal cells. European Journal Of Cell Biology 1994, 65: 327-40. PMID: 7536673.Peer-Reviewed Original ResearchConceptsClone 9 cellsEndocytic compartmentsPotential substrate proteinsDependent protein kinase IISynapsin ISmall intracellular vesiclesProtein kinase IIRat liver endosomesSubstrate proteinsPhosphorylation sequenceNon-neuronal cellsCLIP-170Intracellular vesiclesKinase IILarge endosomesPostnuclear supernatantEndosomesSensitive compartmentLiver endosomesProteinConfocal microscopyCell linesVesiclesIntestinal enterocytesCompartments