Reconstitution of Calcium-Regulated Parathyroid Hormone Secretion from Streptolysin-O-Permeabilized Parathyroid Cells by Guanosine 5′-O-(Thio)Triphosphate*
Matovcik L, Rhee S, Schaefer J, Kinder B. Reconstitution of Calcium-Regulated Parathyroid Hormone Secretion from Streptolysin-O-Permeabilized Parathyroid Cells by Guanosine 5′-O-(Thio)Triphosphate*. Endocrinology 1997, 138: 1170-1179. DOI: 10.1210/en.138.3.1170.Peer-Reviewed Original ResearchIntracellular Ca2+ levelsPTH secretionParathyroid cellsCa2+ levelsPhysiological intracellular Ca2+ levelsHormone secretionRate of PTH secretionCalcium/calmodulin-dependent protein kinase IIIncreased PTH secretionInhibit PTH secretionParathyroid hormone secretionExtracellular Ca2+Regulation of hormone secretionCa2+ concentrationDose-dependent mannerProtein kinase IICa2+-dependent regulationAmbient Ca2+ concentrationStreptolysin OIn vitro model systemPTH releaseIntracellular Ca2SLO-permeabilized cellsKinase IISecretion