2001
ClpA mediates directional translocation of substrate proteins into the ClpP protease
Reid B, Fenton W, Horwich A, Weber-Ban E. ClpA mediates directional translocation of substrate proteins into the ClpP protease. Proceedings Of The National Academy Of Sciences Of The United States Of America 2001, 98: 3768-3772. PMID: 11259663, PMCID: PMC31127, DOI: 10.1073/pnas.071043698.Peer-Reviewed Original ResearchConceptsSubstrate proteinsClpP proteaseUnfolded substrate proteinsATP-dependent unfoldingATP-dependent mannerATP-dependent translocationChaperone ClpAProteolytic chamberFluorescence resonance energy transferDirectional translocationCOOH terminusClpAResonance energy transferProteinTranslocationIntracellular degradationFluorescence anisotropyProteaseRing complexTerminusLarge assembliesDonor fluorophoreRecognition elementRecent studiesHslUV
1999
Global unfolding of a substrate protein by the Hsp100 chaperone ClpA
Weber-Ban E, Reid B, Miranker A, Horwich A. Global unfolding of a substrate protein by the Hsp100 chaperone ClpA. Nature 1999, 401: 90-93. PMID: 10485712, DOI: 10.1038/43481.Peer-Reviewed Original ResearchConceptsSubstrate proteinsATP-dependent degradationGreen fluorescent protein GFPHydrogen exchange experimentsStable monomeric proteinFluorescent protein GFPNon-native formsChaperone ClpAChaperone familyEukaryotic proteinsProtease ClpPPresence of ATPChaperonin GroELHexameric ringClpAProteasome functionProtein GFPProtein structureMonomeric proteinNative proteinGlobal unfoldingProteinCentral channelRecognition peptideClpAP
1985
Arginine in the leader peptide is required for both import and proteolytic cleavage of a mitochondrial precursor.
Horwich A, Kalousek F, Rosenberg L. Arginine in the leader peptide is required for both import and proteolytic cleavage of a mitochondrial precursor. Proceedings Of The National Academy Of Sciences Of The United States Of America 1985, 82: 4930-4933. PMID: 3895227, PMCID: PMC390471, DOI: 10.1073/pnas.82.15.4930.Peer-Reviewed Original ResearchConceptsLeader peptideOrnithine transcarbamoylaseImport of precursorsMost mitochondrial proteinsMitochondrial matrix fractionOverall amino acid compositionMitochondrial matrix enzymeMitochondrial precursorsMitochondrial proteinsSubunit precursorAmino acid compositionBasic arginine residuesBasic residuesMatrix enzymeGlycine residueLarger precursorArginine residuesMatrix fractionIntact mitochondriaNH2-terminalDependent proteaseProteolytic cleavageTranscarbamoylaseResiduesMitochondria