1990
Sorting pathways of mitochondrial inner membrane proteins
MAHLKE K, PFANNER N, MARTIN J, HORWICH A, HARTL F, NEUPERT W. Sorting pathways of mitochondrial inner membrane proteins. The FEBS Journal 1990, 192: 551-555. PMID: 2145157, DOI: 10.1111/j.1432-1033.1990.tb19260.x.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceBiological EvolutionDNA, FungalHeat-Shock ProteinsIntracellular MembranesMitochondrial ADP, ATP TranslocasesMolecular Sequence DataNeurospora crassaOligonucleotide ProbesProtein Processing, Post-TranslationalProton-Translocating ATPasesRecombinant Fusion ProteinsSubmitochondrial ParticlesConceptsMitochondrial inner membrane proteinADP/ATP carrierInner membrane proteinMembrane proteinsATP carrierTargeting signalsNuclear-encoded mitochondrial inner membrane proteinsAmino-terminal targeting signalsNuclear-encoded mitochondrial proteinsDifferent import receptorsMitochondrial precursor proteinsHeat shock protein Hsp60Precursor proteinProkaryotic equivalentProkaryotic ancestorsEndosymbiont hypothesisImport receptorSubunit 9Sorting pathwaysMitochondrial proteinsInner membraneF0-ATPaseMitochondrial matrixAssembly pathwayMitochondrial membrane
1985
Arginine in the leader peptide is required for both import and proteolytic cleavage of a mitochondrial precursor.
Horwich A, Kalousek F, Rosenberg L. Arginine in the leader peptide is required for both import and proteolytic cleavage of a mitochondrial precursor. Proceedings Of The National Academy Of Sciences Of The United States Of America 1985, 82: 4930-4933. PMID: 3895227, PMCID: PMC390471, DOI: 10.1073/pnas.82.15.4930.Peer-Reviewed Original ResearchConceptsLeader peptideOrnithine transcarbamoylaseImport of precursorsMost mitochondrial proteinsMitochondrial matrix fractionOverall amino acid compositionMitochondrial matrix enzymeMitochondrial precursorsMitochondrial proteinsSubunit precursorAmino acid compositionBasic arginine residuesBasic residuesMatrix enzymeGlycine residueLarger precursorArginine residuesMatrix fractionIntact mitochondriaNH2-terminalDependent proteaseProteolytic cleavageTranscarbamoylaseResiduesMitochondriaA leader peptide is sufficient to direct mitochondrial import of a chimeric protein.
Horwich A, Kalousek F, Mellman I, Rosenberg L. A leader peptide is sufficient to direct mitochondrial import of a chimeric protein. The EMBO Journal 1985, 4: 1129-1135. PMID: 3891325, PMCID: PMC554314, DOI: 10.1002/j.1460-2075.1985.tb03750.x.Peer-Reviewed Original ResearchConceptsChimeric precursor proteinsMitochondrial importLeader peptideChimeric precursorsDihydrofolate reductaseMost mitochondrial proteinsPost-translational importMutant CHO cell linesCloned nucleotide sequencePrecursor proteinOrnithine transcarbamylaseCell-free systemCHO cell linesEnzyme dihydrofolate reductaseMitochondrial proteinsMitochondrial localizationRegulatory elementsLeader sequenceNucleotide sequenceStable transformantsAdditional proteinsSelectable markerChimeric proteinLarger precursorIntact cells
1984
Structure and Expression of a Complementary DNA for the Nuclear Coded Precursor of Human Mitochondrial Ornithine Transcarbamylase
Horwich A, Fenton W, Williams K, Kalousek F, Kraus J, Doolittle R, Konigsberg W, Rosenberg L. Structure and Expression of a Complementary DNA for the Nuclear Coded Precursor of Human Mitochondrial Ornithine Transcarbamylase. Science 1984, 224: 1068-1074. PMID: 6372096, DOI: 10.1126/science.6372096.Peer-Reviewed Original ResearchConceptsComplementary DNALeader peptideOrnithine transcarbamylaseAmino-terminal leader peptideMost mitochondrial proteinsComplete primary structureHuman ornithine transcarbamylaseFree cytoplasmic ribosomesMitochondrial matrix enzymeCultured HeLa cellsMitochondrial proteinsCytoplasmic ribosomesRegulatory elementsNucleotide sequenceStable transformantsMatrix enzymeAsparagine residuesAcidic residuesLarger precursorMature formPrimary structureProtein occursHeLa cellsEscherichia coliAmino acids