Import and processing of human ornithine transcarbamoylase precursor by mitochondria from Saccharomyces cerevisiae.
Cheng M, Pollock R, Hendrick J, Horwich A. Import and processing of human ornithine transcarbamoylase precursor by mitochondria from Saccharomyces cerevisiae. Proceedings Of The National Academy Of Sciences Of The United States Of America 1987, 84: 4063-4067. PMID: 3295876, PMCID: PMC305022, DOI: 10.1073/pnas.84.12.4063.Peer-Reviewed Original ResearchConceptsMitochondrial membraneEnzymatic activityNH2-terminal leader peptideMitochondrial matrix fractionWild-type precursorS. cerevisiae strainMitochondrial importMammalian mitochondriaMature subunitSubunit precursorOperon promoterS. cerevisiaeSelective growth conditionsLeader peptideYeast cellsArtificial mutationsOTCase activityMatrix fractionOrnithine transcarbamoylaseCerevisiae strainSaccharomycesGrowth conditionsMatrix compartmentMitochondriaSubunits