2001
Allostery and protein substrate conformational change during GroEL/GroES-mediated protein folding
Saibil H, Horwich A, Fenton W. Allostery and protein substrate conformational change during GroEL/GroES-mediated protein folding. Advances In Protein Chemistry 2001, 59: 45-72. PMID: 11868280, DOI: 10.1016/s0065-3233(01)59002-6.Peer-Reviewed Original ResearchConceptsProtein foldingATP-dependent protein foldingChloroplasts of eukaryotesDouble-ring complexesCo-chaperonin GroESC-terminal portionChaperonin machineProtein folding reactionChaperonin systemSubstrate polypeptidesChaperonin complexGroEL-GroESHeptameric ringsGroEL subunitStructural biologyBiophysical approachesEquatorial domainATPase mechanismConformational changesSubstrate conformational changesFolding reactionNative formGroESFoldingGroEL
1996
Characterization of the Active Intermediate of a GroEL–GroES-Mediated Protein Folding Reaction
Weissman J, Rye H, Fenton W, Beechem J, Horwich A. Characterization of the Active Intermediate of a GroEL–GroES-Mediated Protein Folding Reaction. Cell 1996, 84: 481-490. PMID: 8608602, DOI: 10.1016/s0092-8674(00)81293-3.Peer-Reviewed Original ResearchConceptsCis ternary complexProtein foldingRelease of GroESAddition of GroESFolding reactionTernary complexNonhydrolyzable ATP analogGroES releaseProtein folding reactionSubstrate proteinsPresence of ATPGroEL mutantGroEL-GroESGroEL complexNonnative substratesATP hydrolysisGroESComplete foldingSubstrate flexibilityATP analogFoldingFluorescence anisotropyActive stateATPRecent studies
1994
GroEL-mediated protein folding proceeds by multiple rounds of binding and release of nonnative forms
Weissman J, Kashi Y, Fenton W, Horwich A. GroEL-mediated protein folding proceeds by multiple rounds of binding and release of nonnative forms. Cell 1994, 78: 693-702. PMID: 7915201, DOI: 10.1016/0092-8674(94)90533-9.Peer-Reviewed Original ResearchConceptsCochaperonin GroESMultiple roundsGroEL functionChaperonin GroELKinetic partitioningMutant formsNonnative conformationsNonnative formsGroELAddition of ATPGroEL moleculeTryptophan fluorescenceFolding reactionDouble-ring structureUnfolded statePolypeptideDiverse setGroESProteolysisProteinATPBindingFateConformationComplexes