2020
Functional interactions of ion channels with the actin cytoskeleton: does coupling to dynamic actin regulate NMDA receptors?
Shaw JE, Koleske AJ. Functional interactions of ion channels with the actin cytoskeleton: does coupling to dynamic actin regulate NMDA receptors? The Journal Of Physiology 2020, 599: 431-441. PMID: 32034761, PMCID: PMC7416480, DOI: 10.1113/jp278702.Peer-Reviewed Original ResearchConceptsActin cytoskeletonIon channelsPostsynaptic compartmentsCytoskeletal protein actinActin binding proteinsNeurotransmitter release machineryNeurotransmitter receptorsProtein actinSynaptic neurotransmitter receptorsΑ-actininMolecular mechanismsBinding proteinCytoskeletonMolecular linkageFunctional interactionRelease machineryDiverse collectionActinNMDA receptorsTraffickingChannel openingProteinVoltage-gated NaReceptorsCompartments
2018
Noonan Syndrome-Associated SHP2 Dephosphorylates GluN2B to Regulate NMDA Receptor Function
Levy AD, Xiao X, Shaw JE, Devi S, Katrancha SM, Bennett AM, Greer CA, Howe JR, Machida K, Koleske AJ. Noonan Syndrome-Associated SHP2 Dephosphorylates GluN2B to Regulate NMDA Receptor Function. Cell Reports 2018, 24: 1523-1535. PMID: 30089263, PMCID: PMC6234505, DOI: 10.1016/j.celrep.2018.07.006.Peer-Reviewed Original ResearchConceptsTyrosine phosphatase SHP2Noonan syndromePhosphatase SHP2Regulatory proteinsSHP2Recombinant GluN1Nck2Receptor functionNMDA receptor functionNMDAR functionGluN2B functionMutationsNMDAR dysfunctionNeuron functionNS miceGluN1ProteinAllelesNMDA receptorsDiheteromersReceptor kineticsReduced contributionsFunctionHyperactivationMice
2016
Disruption of Coordinated Presynaptic and Postsynaptic Maturation Underlies the Defects in Hippocampal Synapse Stability and Plasticity in Abl2/Arg-Deficient Mice
Xiao X, Levy AD, Rosenberg BJ, Higley MJ, Koleske AJ. Disruption of Coordinated Presynaptic and Postsynaptic Maturation Underlies the Defects in Hippocampal Synapse Stability and Plasticity in Abl2/Arg-Deficient Mice. Journal Of Neuroscience 2016, 36: 6778-6791. PMID: 27335408, PMCID: PMC4916252, DOI: 10.1523/jneurosci.4092-15.2016.Peer-Reviewed Original ResearchMeSH KeywordsAge FactorsAnimalsAnimals, NewbornDendritic SpinesExcitatory Amino Acid AgentsFemaleGene Expression Regulation, DevelopmentalGlutamic AcidHippocampusHSP70 Heat-Shock ProteinsIn Vitro TechniquesMaleMiceMice, Inbred C57BLMice, KnockoutNeuronal PlasticityNeurotransmitter AgentsProtein-Tyrosine KinasesReceptors, GlutamateReceptors, N-Methyl-D-AspartateSubcellular FractionsSynapsesSynaptic PotentialsConceptsNMDA receptorsHippocampal synapsesEarly adulthoodFocal glutamate uncagingImmature glutamatergic synapsesDendritic spine numberNormal synaptic plasticityPostnatal day 21NMDA receptor currentsLate postnatal developmentHigh release probabilityArg kinaseForms of plasticityGlutamate uncagingSynapse lossPresynaptic sitesGlutamatergic synapsesSynapse stabilityPostsynaptic sitesSynaptic stabilityCultured neuronsMice ageReceptor currentsDay 21Synaptic plasticity