2023
Direct determination of oligomeric organization of integral membrane proteins and lipids from intact customizable bilayer
Panda A, Giska F, Duncan A, Welch A, Brown C, McAllister R, Hariharan P, Goder J, Coleman J, Ramakrishnan S, Pincet F, Guan L, Krishnakumar S, Rothman J, Gupta K. Direct determination of oligomeric organization of integral membrane proteins and lipids from intact customizable bilayer. Nature Methods 2023, 20: 891-897. PMID: 37106230, PMCID: PMC10932606, DOI: 10.1038/s41592-023-01864-5.Peer-Reviewed Original ResearchConceptsIntegral membrane proteinsMembrane proteinsOligomeric organizationOligomeric stateNative mass spectrometry analysisFunctional oligomeric stateKey membrane componentMass spectrometry analysisNMS analysisTarget membraneLipid bindingMembrane componentsProteolipid vesiclesMembrane compositionLipid compositionSpectrometry analysisLipid membranesNeurotransmitter releaseProteinMembraneLipidsMembrane propertiesDirect determinationBilayersTransporters
2015
RDGBα, a PtdIns-PtdOH transfer protein, regulates G-protein-coupled PtdIns(4,5)P2 signalling during Drosophila phototransduction
Yadav S, Garner K, Georgiev P, Li M, Gomez-Espinosa E, Panda A, Mathre S, Okkenhaug H, Cockcroft S, Raghu P. RDGBα, a PtdIns-PtdOH transfer protein, regulates G-protein-coupled PtdIns(4,5)P2 signalling during Drosophila phototransduction. Journal Of Cell Science 2015, 128: 3330-3344. PMID: 26203165, PMCID: PMC4582195, DOI: 10.1242/jcs.173476.Peer-Reviewed Original ResearchConceptsPhospholipase CPlasma membraneEndoplasmic reticulumMulti-domain proteinsPlasma membrane lipidsExchange of lipidsRdgB functionDrosophila phototransductionDrosophila photoreceptorsProtein domainsRdgB mutantsVivo functionMembrane lipidsPtdInsLipid intermediatesLipid homeostasisMembrane receptorsPLC activationPtdOH levelsTransfer proteinPhosphatidic acidPLC activityPhototransductionNormal phototransductionPtdOH