2012
Transient mGlu5R inhibition enhances the survival of granule cell precursors in the neonatal cerebellum
Kubera C, Hernandez AL, Heng V, Bordey A. Transient mGlu5R inhibition enhances the survival of granule cell precursors in the neonatal cerebellum. Neuroscience 2012, 219: 271-279. PMID: 22677205, PMCID: PMC3402690, DOI: 10.1016/j.neuroscience.2012.05.064.Peer-Reviewed Original ResearchConceptsExternal germinal layerGranule cell precursorsGranule cellsS-phase marker bromodeoxyuridineMetabotropic glutamate receptor 5Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) stainingTransferase dUTP nick end labeling stainingCell precursorsDUTP nick end labeling stainingNick end labeling stainingGlutamate receptor 5Postnatal day 2Proliferative granule cell precursorsEnd labeling stainingCerebellar granule cellsAcute slicesAbundant neuronsBrdU injectionLabeling stainingVivo treatmentReceptor 5Day 2Vivo injectionSpecific receptorsClonal expansion
2010
Astroglial cells in the external granular layer are precursors of cerebellar granule neurons in neonates
Silbereis J, Heintz T, Taylor MM, Ganat Y, Ment LR, Bordey A, Vaccarino F. Astroglial cells in the external granular layer are precursors of cerebellar granule neurons in neonates. Molecular And Cellular Neuroscience 2010, 44: 362-373. PMID: 20470892, PMCID: PMC2900521, DOI: 10.1016/j.mcn.2010.05.001.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornAstrocytesBasic Helix-Loop-Helix Transcription FactorsBeta-GalactosidaseCell LineageCerebellumGenes, ReporterGlial Fibrillary Acidic ProteinGreen Fluorescent ProteinsIntegrasesMiceMice, Inbred C57BLMice, TransgenicNeurogenesisNeuronsPromoter Regions, GeneticStem CellsTime FactorsConceptsExternal granule cell layerGranule cell precursorsInternal granule cell layerGranule cell layerGranule cellsRhombic lipAstroglial cellsProtein expression profilesGlial fibrillary acidic protein promoterCerebellar granule cell precursorsHuman glial fibrillary acidic protein promoterEmbryonic rhombic lipInducible Cre recombinaseNeuronal progenitor cellsReporter proteinFirst postnatal weekNeural stem cell markersLate embryogenesisCellular plasticityImmature granule cellsEarly postnatal developmentCell layerReporter geneCerebellar granule neuronsStem cell markers
2009
GABA increases Ca2+ in cerebellar granule cell precursors via depolarization: Implications for proliferation
Dave KA, Bordey A. GABA increases Ca2+ in cerebellar granule cell precursors via depolarization: Implications for proliferation. IUBMB Life 2009, 61: 496-503. PMID: 19391160, PMCID: PMC2675662, DOI: 10.1002/iub.185.Peer-Reviewed Original ResearchConceptsGamma-aminobutyric acidRole of GABAVertebrate central nervous systemComplement of receptorsCerebellar granule cell precursorsCell precursorsGranule cell precursorsDevelopmental processesMetabotropic glutamate receptorsCentral nervous systemCell proliferationAdult mammalsNovel targetAmino acid glutamatePharmacological regulationCurrent knowledgeGlutamate receptorsNeural cancersNervous systemCerebellar tumorsCalcium increaseReceptorsProliferationMammalsNovel data
2004
Bergmann glial GlyT1 mediates glycine uptake and release in mouse cerebellar slices
Huang H, Barakat L, Wang D, Bordey A. Bergmann glial GlyT1 mediates glycine uptake and release in mouse cerebellar slices. The Journal Of Physiology 2004, 560: 721-736. PMID: 15331688, PMCID: PMC1665288, DOI: 10.1113/jphysiol.2004.067801.Peer-Reviewed Original ResearchConceptsWhole-cell patch-clamp recordingsGlycine receptor blockerMouse cerebellar slicesNMDA receptor activationVoltage-dependent inward currentsExtracellular glycine levelsPatch-clamp recordingsDependent glycine transportersReceptor blockersInhibitory neurotransmitterNMDA receptorsGlycine levelsBergmann gliaCerebellar slicesInward currentsReceptor activationGlycine transporterGliaGlycine transportGlyT1Glycine uptakeBlockersAgonistsIntact systemNeuronesGlial Glutamate Transporters Limit Spillover Activation of Presynaptic NMDA Receptors and Influence Synaptic Inhibition of Purkinje Neurons
Huang H, Bordey A. Glial Glutamate Transporters Limit Spillover Activation of Presynaptic NMDA Receptors and Influence Synaptic Inhibition of Purkinje Neurons. Journal Of Neuroscience 2004, 24: 5659-5669. PMID: 15215288, PMCID: PMC6729224, DOI: 10.1523/jneurosci.1338-04.2004.Peer-Reviewed Original ResearchMeSH KeywordsAction PotentialsAmino Acid Transport System X-AGAnimalsAntiportersCalciumCerebellumCystineEvoked PotentialsGamma-Aminobutyric AcidGlutamic AcidIn Vitro TechniquesMiceNerve FibersNeural InhibitionNeurogliaPatch-Clamp TechniquesPurkinje CellsReceptors, N-Methyl-D-AspartateReceptors, PresynapticRyanodineSynaptic TransmissionConceptsGlial glutamate transportersMiniature IPSCsPurkinje neuronsGlutamate transportersSpontaneous IPSCsSynaptic inhibitionInhibitory synapsesNMDAR activationWhole-cell patch-clamp recordingsPresynaptic NMDA receptorsPaired-pulse ratioMouse cerebellar slicesNMDA receptor activationPresynaptic NMDA receptor activationPatch-clamp recordingsBergmann glial cellsBeta-benzyloxyaspartic acidAdjacent Purkinje neuronsCystine-glutamate antiporterMIPSC frequencyGABAergic terminalsGABAergic synapsesGlial cellsNMDA receptorsExcitatory synapses
2002
GAT-1 and Reversible GABA Transport in Bergmann Glia in Slices
Barakat L, Bordey A. GAT-1 and Reversible GABA Transport in Bergmann Glia in Slices. Journal Of Neurophysiology 2002, 88: 1407-1419. PMID: 12205162, DOI: 10.1152/jn.2002.88.3.1407.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiological TransportCarrier ProteinsCerebellumElectrophysiologyGABA Plasma Membrane Transport ProteinsGamma-Aminobutyric AcidIn Vitro TechniquesMembrane ProteinsMembrane Transport ProteinsNeurogliaOrganic Anion TransportersPatch-Clamp TechniquesRatsRats, Sprague-DawleyReceptors, GABA-AConceptsGAT-1Bergmann gliaInward currentsGABA transporterWhole-cell patch-clamp recordingsCell patch-clamp recordingsGlial GABA uptakePatch-clamp recordingsRat cerebellar slicesGABA perfusionReceptor blockersAmbient GABANNC-711Extracellular GABAGABA effluxGABA uptakeGABA receptorsCerebellar slicesGAT subtypesNipecotic acidReceptor activationGABABlockersGliaOutward currentsCarrier‐mediated uptake and release of taurine from Bergmann glia in rat cerebellar slices
Barakat L, Wang D, Bordey A. Carrier‐mediated uptake and release of taurine from Bergmann glia in rat cerebellar slices. The Journal Of Physiology 2002, 541: 753-767. PMID: 12068038, PMCID: PMC2290349, DOI: 10.1113/jphysiol.2001.015834.Peer-Reviewed Original ResearchConceptsRat cerebellar slicesBergmann gliaGuanidinoethyl sulphonateCerebellar slicesTaurine transporterInward currentsWhole-cell patch-clamp recordingsTaurine uptakeGABA receptor blockersTaurine transporter inhibitorRelease of taurinePatch-clamp recordingsDependent taurine transporterOrder of potencyTaurine perfusionReceptor blockersIntracellular taurineIschemic conditionsCarrier-mediated uptakeTaurine effluxOutward currentsTransporter currentsTransporter inhibitorsIntracellular perfusionGlia