Featured Publications
Ectopic HCN4 expression drives mTOR-dependent epilepsy in mice
Hsieh LS, Wen JH, Nguyen LH, Zhang L, Getz SA, Torres-Reveron J, Wang Y, Spencer DD, Bordey A. Ectopic HCN4 expression drives mTOR-dependent epilepsy in mice. Science Translational Medicine 2020, 12 PMID: 33208499, PMCID: PMC9888000, DOI: 10.1126/scitranslmed.abc1492.Peer-Reviewed Original ResearchConceptsFocal cortical dysplasia type IITuberous sclerosis complexFocal cortical malformationsPyramidal neuronsMouse modelHCN4 expressionCortical pyramidal neuronsOnset of seizuresIntracellular cAMP concentrationSeizure activityCortical malformationsRepetitive firingDiseased neuronsSeizuresAbnormal expressionNeuronsEpilepsyCausative linkSeizure mechanismsCAMP concentrationMechanistic targetHCN4Channel activityPatientsGene therapy
2006
GFAP‐expressing cells in the postnatal subventricular zone display a unique glial phenotype intermediate between radial glia and astrocytes
Liu X, Bolteus AJ, Balkin DM, Henschel O, Bordey A. GFAP‐expressing cells in the postnatal subventricular zone display a unique glial phenotype intermediate between radial glia and astrocytes. Glia 2006, 54: 394-410. PMID: 16886203, DOI: 10.1002/glia.20392.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid Transport System X-AGAnimalsAnimals, NewbornAstrocytesBiomarkersCell DifferentiationCell ShapeConnexinsEpendymaGlial Fibrillary Acidic ProteinGlutamic AcidGreen Fluorescent ProteinsMembrane PotentialsMiceMice, TransgenicOrgan Culture TechniquesPatch-Clamp TechniquesPhenotypePotassiumPotassium ChannelsRecombinant Fusion ProteinsStem CellsTelencephalonConceptsGlial fibrillary acidic proteinPostnatal subventricular zoneSubventricular zoneGFAP-expressing cellsRadial gliaAstroglial marker glial fibrillary acidic proteinGlial propertiesEpendymal cellsGlutamate transportersGLT-1 glutamate transporterMarker glial fibrillary acidic proteinAMPA-type glutamate receptorsFunctional glutamate transportersFibrillary acidic proteinHuman glial fibrillary acidic proteinAdult subventricular zoneConnexin 43 expressionGap junction couplingNeural stem cellsMicroM Ba2Acute slicesAstrocytic functionsGlutamate receptorsGlial phenotypeClamp recordings
2003
Chemokine modulation of high‐conductance Ca2+‐sensitive K+ currents in microglia from human hippocampi
Bordey A, Spencer DD. Chemokine modulation of high‐conductance Ca2+‐sensitive K+ currents in microglia from human hippocampi. European Journal Of Neuroscience 2003, 18: 2893-2898. PMID: 14656339, DOI: 10.1111/j.1460-9568.2003.03021.x.Peer-Reviewed Original ResearchConceptsHigh-conductance Ca2MIP1-alphaAcute pathological processesNon-activated microgliaSensitive outward currentsPharmaco-resistant epilepsyPatch-clamp recordingsOutward current amplitudeHigh input resistanceChemokine modulationHippocampal slicesInjury siteBrain slicesChronic pathologiesMicrogliaEpileptic patientsAmeboid morphologyIntracellular Ca2Human hippocampusOutward currentsMigratory effectsInput resistancePositive labelingPathological processesMode of actionGABA Depolarizes Neuronal Progenitors of the Postnatal Subventricular Zone Via GABAA Receptor Activation
Wang DD, Krueger DD, Bordey A. GABA Depolarizes Neuronal Progenitors of the Postnatal Subventricular Zone Via GABAA Receptor Activation. The Journal Of Physiology 2003, 550: 785-800. PMID: 12807990, PMCID: PMC2343064, DOI: 10.1113/jphysiol.2003.042572.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAnimalsBarbituratesBenzodiazepinesCerebral VentriclesDose-Response Relationship, DrugElectrophysiologyGABA AntagonistsGamma-Aminobutyric AcidGlutamate DecarboxylaseIsoenzymesMembrane PotentialsMiceNeostriatumNeuronsPatch-Clamp TechniquesPicrotoxinPotassium ChannelsReceptors, GABA-AStem CellsZincConceptsRostral migratory streamGABAA receptor activationSubventricular zoneNeuronal progenitorsReceptor activationGlutamic acid decarboxylase 67Resting potentialsPostnatal mammalian brainPatch-clamp recordingsSVZ/RMSMouse brain slicesRecorded cellsCell-attached patchesGABA responsesBrain slicesNeurotransmitter GABASubset of progenitorsBenzodiazepine agonistsEmbryonic neuronsAction potentialsMammalian brainMigratory streamGABAInverse agonistSpecific receptors
2002
Analysis of the K+ Current Profile of Mature Rat Oligodendrocytes in situ
Gipson K, Bordey A. Analysis of the K+ Current Profile of Mature Rat Oligodendrocytes in situ. The Journal Of Membrane Biology 2002, 189: 201-212. PMID: 12395285, DOI: 10.1007/s00232-002-1014-8.Peer-Reviewed Original Research
2001
Electrophysiological Characteristics of Reactive Astrocytes in Experimental Cortical Dysplasia
Bordey A, Lyons SA, Hablitz JJ, Sontheimer H. Electrophysiological Characteristics of Reactive Astrocytes in Experimental Cortical Dysplasia. Journal Of Neurophysiology 2001, 85: 1719-1731. PMID: 11287494, DOI: 10.1152/jn.2001.85.4.1719.Peer-Reviewed Original ResearchConceptsGap junction couplingReactive gliosisFreeze lesionGlial fibrillary acidic protein immunoreactivityWhole-cell patch-clamp recordingsCell patch-clamp recordingsExperimental cortical dysplasiaCortical layers IPatch-clamp recordingsPostnatal day 16Reduced gap junction couplingNormal neuronal functionDysplastic neocortexP24 ratsDysplastic cortexReactive astrocytesCortical dysplasiaNeuronal dysfunctionProtein immunoreactivityElectrophysiological characteristicsAstrocytesChannel expressionNeuronal functionMicrosulcusLayer I
2000
Reactive astrocytes show enhanced inwardly rectifying K+ currents in situ
Bordey A, Hablitz JJ, Sontheimer H. Reactive astrocytes show enhanced inwardly rectifying K+ currents in situ. Neuroreport 2000, 11: 3151-3155. PMID: 11043540, DOI: 10.1097/00001756-200009280-00022.Peer-Reviewed Original ResearchMuscarinic Activation of BK Channels Induces Membrane Oscillations in Glioma Cells and Leads to Inhibition of Cell Migration
Bordey A, Sontheimer H, Trouslard J. Muscarinic Activation of BK Channels Induces Membrane Oscillations in Glioma Cells and Leads to Inhibition of Cell Migration. The Journal Of Membrane Biology 2000, 176: 31-40. PMID: 10882426, DOI: 10.1007/s00232001073.Peer-Reviewed Original ResearchConceptsPatch-clamp recordingsGlioma cellsAstrocytoma cellsCell migrationCultured human astrocytoma cellsIntracellular free calcium concentrationACh receptor agonistsFree calcium concentrationHuman astrocytoma cellsRelease of Ca2Glioma cell migrationMuscarinic activationMuscarinic antagonistCerebral tumorsMuscarinic receptorsReceptor agonistBath applicationActivate Ca2Intracellular injectionCerebrospinal fluidACh applicationAcetylcholineBiphasic increaseIntracellular storesExternal calciumIon channel expression by astrocytes in situ: Comparison of different CNS regions
Bordey A, Sontheimer H. Ion channel expression by astrocytes in situ: Comparison of different CNS regions. Glia 2000, 30: 27-38. PMID: 10696142, DOI: 10.1002/(sici)1098-1136(200003)30:1<27::aid-glia4>3.0.co;2-#.Peer-Reviewed Original ResearchConceptsBergmann glial cellsIon channel complementGlial cellsIon channel expressionDifferent CNS regionsCNS regionsChannel expressionPercentage of astrocytesTransient A-typeCerebellar Bergmann glial cellsChannel complementPatch-clamp recordingsVoltage-activated currentsPostnatal day 17Whole-cell currentsIon channelsVoltage-gated ion channelsBrain slicesRat astrocytesAstrocytesDay 17Low input resistanceInput resistanceCNSInitial recording
1999
Differential Inhibition of Glial K+ Currents by 4-AP
Bordey A, Sontheimer H. Differential Inhibition of Glial K+ Currents by 4-AP. Journal Of Neurophysiology 1999, 82: 3476-3487. PMID: 10601476, DOI: 10.1152/jn.1999.82.6.3476.Peer-Reviewed Original ResearchMeSH Keywords4-AminopyridineAction PotentialsAnimalsAstrocytesCells, CulturedDelayed Rectifier Potassium ChannelsElectrophysiologyLarge-Conductance Calcium-Activated Potassium ChannelsNeurogliaPatch-Clamp TechniquesPotassium Channel BlockersPotassium ChannelsPotassium Channels, Calcium-ActivatedPotassium Channels, Inwardly RectifyingPotassium Channels, Voltage-GatedRatsRats, Sprague-DawleySpinal CordTetraethylammoniumConceptsAcute spinal cord slicesSpinal cord slicesConcentration-dependent blockPotassium channel typesType currentsDose-dependent inhibitorNeuronal action potentialsCord slicesSpinal cordChannel blockersEpileptiform dischargesBrain slicesAction potentialsHomeostatic functionsA-currentAstrocytesDifferential inhibitionRepolarizationMembrane potentialChannel typesSlicesBlockersGlialCord
1998
Passive Glial Cells, Fact or Artifact?
Bordey A, Sontheimer H. Passive Glial Cells, Fact or Artifact? The Journal Of Membrane Biology 1998, 166: 213-222. PMID: 9843595, DOI: 10.1007/s002329900463.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsVoltage-activated currentsPassive astrocytesVoltage-dependent outwardCell accessAcute tissue slicesBath Ca2Glial cellsRat hippocampusSlice recordingsCultured astrocytesAstrocytesPipette solutionEffect of Ca2Tissue slicesMembrane capacitanceSuch cellsSubpopulationsCellsTransient maskingConcentration of Ca2Ca2SuperfusionHippocampusDoseProperties of human glial cells associated with epileptic seizure foci
Bordey A, Sontheimer H. Properties of human glial cells associated with epileptic seizure foci. Epilepsy Research 1998, 32: 286-303. PMID: 9761328, DOI: 10.1016/s0920-1211(98)00059-x.Peer-Reviewed Original ResearchConceptsSeizure focusGlial cellsMesio-temporal lobe epilepsyWhole-cell patch-clamp recordingsLucifer YellowControl rat hippocampusPathophysiology of seizuresSlow action potentialsCurrent-clamp studiesGFAP-positive astrocytesEpileptic seizure fociPatch-clamp recordingsHuman glial cellsLobe epilepsyAcute slicesRat hippocampusEpilepsy patientsClamp studiesRat astrocytesAstrocytesIntermediate filaments GFAPNormal astrocytesAction potentialsPhysiological propertiesBranched processesElectrophysiological Properties of Human Astrocytic Tumor Cells In Situ: Enigma of Spiking Glial Cells
Bordey A, Sontheimer H. Electrophysiological Properties of Human Astrocytic Tumor Cells In Situ: Enigma of Spiking Glial Cells. Journal Of Neurophysiology 1998, 79: 2782-2793. PMID: 9582244, DOI: 10.1152/jn.1998.79.5.2782.Peer-Reviewed Original ResearchMeSH KeywordsAction PotentialsAstrocytesAstrocytomaBrain NeoplasmsCarcinomaCell DifferentiationChildChoroid Plexus NeoplasmsDelayed Rectifier Potassium ChannelsHumansIon TransportNeoplasm ProteinsNeoplastic Stem CellsNerve Tissue ProteinsPatch-Clamp TechniquesPotassiumPotassium Channel BlockersPotassium ChannelsPotassium Channels, Inwardly RectifyingPotassium Channels, Voltage-GatedSodium Channel BlockersSodium ChannelsSpinal CordTetraethylammoniumTetrodotoxinConceptsAstrocytoma cellsTumor cellsGlial cellsWhole-cell patch-clamp recordingsCell patch-clamp recordingsAction potential-like responsesOlder pediatric patientsSpinal cord astrocytesHuman astrocytic tumor cellsSensitive sodium currentsGroup of tumorsLow-grade astrocytomasPatch-clamp recordingsOutward potassium currentGlial tumor cellsAstrocytic tumor cellsGeneration of spikesHigh input resistancePeritumoral astrocytesPediatric patientsMicroM Ba2Pilocytic astrocytomaPotassium currentAstrocytesElectrophysiological properties
1995
Kinetics of A-currents in sympathetic preganglionic neurones and glial cells
Bordey A, Feltz P, Trouslard J. Kinetics of A-currents in sympathetic preganglionic neurones and glial cells. Neuroreport 1995, 7: 37-40. PMID: 8742411, DOI: 10.1097/00001756-199512290-00008.Peer-Reviewed Original Research