2021
Rab27a-Dependent Paracrine Communication Controls Dendritic Spine Formation and Sensory Responses in the Barrel Cortex
Zhang L, Zhang X, Hsieh LS, Lin TV, Bordey A. Rab27a-Dependent Paracrine Communication Controls Dendritic Spine Formation and Sensory Responses in the Barrel Cortex. Cells 2021, 10: 622. PMID: 33799820, PMCID: PMC8000154, DOI: 10.3390/cells10030622.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCells, CulturedDendritic SpinesExcitatory Postsynaptic PotentialsExtracellular VesiclesFemaleGene Expression Regulation, DevelopmentalGestational AgeMiceParacrine CommunicationPregnancyPyramidal CellsRab27 GTP-Binding ProteinsSensory Receptor CellsSomatosensory CortexSynaptic TransmissionVibrissaeConceptsSmall extracellular vesiclesL4 neuronsParacrine communicationExcitatory synaptic transmissionRelease of sEVsDendritic spine formationCell-autonomous effectsRisk of autismL2/3 neuronsPyramidal neuronsLayer 2/3Somatosensory cortexBarrel cortexCortical neuronsSynaptic transmissionWhisker stimulationSomatosensory informationJuvenile miceSynaptic connectivitySynaptic integrationSpine formationBrain developmentNeuronsSensory stimulationSpine development
2019
Hypervascularization in mTOR‐dependent focal and global cortical malformations displays differential rapamycin sensitivity
Zhang L, Huang T, Teaw S, Bordey A. Hypervascularization in mTOR‐dependent focal and global cortical malformations displays differential rapamycin sensitivity. Epilepsia 2019, 60: 1255-1265. PMID: 31125447, PMCID: PMC6558978, DOI: 10.1111/epi.15969.Peer-Reviewed Original ResearchConceptsBlood vesselsRapamycin treatmentVessel densityVessel abnormalitiesPostnatal day 14 miceAbsence of seizuresWild-type miceConditional transgenic miceTuberous sclerosis complexTypes of MCDDay 14 miceMCD modelFocal MCDMTOR blockersDysplastic neuronsFunctional outcomeEpilepsy treatmentSomatosensory cortexYoung miceFocal malformationsCortical developmentJuvenile miceTotal vessel lengthAnimal modelsTransgenic mice
2018
In utero electroporation-based translating ribosome affinity purification identifies age-dependent mRNA expression in cortical pyramidal neurons
Huang T, Nguyen L, Lin TV, Gong X, Zhang L, Kim GB, Sarkisian MR, Breunig JJ, Bordey A. In utero electroporation-based translating ribosome affinity purification identifies age-dependent mRNA expression in cortical pyramidal neurons. Neuroscience Research 2018, 143: 44-52. PMID: 29857015, PMCID: PMC6265126, DOI: 10.1016/j.neures.2018.05.006.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBrainDisks Large Homolog 4 ProteinDoublecortin ProteinElectroporationEmbryonic DevelopmentFemaleGene Expression ProfilingGene Expression Regulation, DevelopmentalGreen Fluorescent ProteinsMaleMiceOligonucleotide Array Sequence AnalysisProtein BiosynthesisPyramidal CellsRecombinant Fusion ProteinsRibosomal Protein L10Ribosomal ProteinsRNA, MessengerSomatosensory CortexSynapsinsConceptsSpecific neuronal populationsRibosomal protein L10aRibosome affinity purificationSelective biological processesNeuronal genesMolecular profileSpecific developmental patternsTarget genesAffinity purificationBiological processesGenesUtero electroporationNeuronal populationsEmbryonic day 14Molecular profilingDevelopmental patternsMRNA expression