Featured Publications
Expression of 4E-BP1 in juvenile mice alleviates mTOR-induced neuronal dysfunction and epilepsy
Nguyen LH, Xu Y, Mahadeo T, Zhang L, Lin TV, Born HA, Anderson AE, Bordey A. Expression of 4E-BP1 in juvenile mice alleviates mTOR-induced neuronal dysfunction and epilepsy. Brain 2021, 145: 1310-1325. PMID: 34849602, PMCID: PMC9128821, DOI: 10.1093/brain/awab390.Peer-Reviewed Original ResearchConceptsFocal malformationsCortical developmentJuvenile miceCortical spectral activitySpontaneous seizure frequencyIrregular firing patternNovel therapeutic opportunitiesNeuronal cytomegalySeizure frequencyEpilepsy onsetNeuronal abnormalitiesElectrophysiological alterationsIntractable epilepsyNeuronal dysfunctionJuvenile brainMouse modelMTOR pathwayTherapeutic opportunitiesMalformationsFiring patternsEpilepsyAberrant expressionNeurodevelopmental disordersMTOR effectorsMice
2022
Loss of PTEN phosphorylation via single point mutation alters cortical connectivity and behaviour
Binder M, Bordey A. Loss of PTEN phosphorylation via single point mutation alters cortical connectivity and behaviour. Brain 2022, 145: 3343-3344. PMID: 36148582, PMCID: PMC10233246, DOI: 10.1093/brain/awac350.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus Statements
2016
Mammalian FMRP S499 Is Phosphorylated by CK2 and Promotes Secondary Phosphorylation of FMRP
Bartley CM, O’Keefe R, Blice-Baum A, Mihailescu MR, Gong X, Miyares L, Karaca E, Bordey A. Mammalian FMRP S499 Is Phosphorylated by CK2 and Promotes Secondary Phosphorylation of FMRP. ENeuro 2016, 3: eneuro.0092-16.2016. PMID: 27957526, PMCID: PMC5116651, DOI: 10.1523/eneuro.0092-16.2016.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlotting, WesternCasein Kinase IICells, CulturedCerebral CortexFragile X Mental Retardation ProteinHEK293 CellsHumansMass SpectrometryMiceNaphthyridinesPhenazinesPhosphorylationProtein BiosynthesisProtein Kinase InhibitorsReceptors, Metabotropic GlutamateRecombinant ProteinsTime FactorsConceptsTranslational repressionNearby residuesProtein phosphatase 2ACasein kinase IIMental retardation proteinFMRP lossPhosphatase 2AFragile X syndromePP2A pathwaySecondary phosphorylationProtein translationKinase IIGene transcriptsFMRPBrain transcriptsFunction mutationsPhosphorylationS499X syndromeTranscriptsRepressionResiduesRecent evidenceCK2Kinase
2014
FMRP S499 Is Phosphorylated Independent of mTORC1-S6K1 Activity
Bartley CM, O’Keefe R, Bordey A. FMRP S499 Is Phosphorylated Independent of mTORC1-S6K1 Activity. PLOS ONE 2014, 9: e96956. PMID: 24806451, PMCID: PMC4013076, DOI: 10.1371/journal.pone.0096956.Peer-Reviewed Original Research
2013
mTORC1 Targets the Translational Repressor 4E-BP2, but Not S6 Kinase 1/2, to Regulate Neural Stem Cell Self-Renewal In Vivo
Hartman NW, Lin TV, Zhang L, Paquelet GE, Feliciano DM, Bordey A. mTORC1 Targets the Translational Repressor 4E-BP2, but Not S6 Kinase 1/2, to Regulate Neural Stem Cell Self-Renewal In Vivo. Cell Reports 2013, 5: 433-444. PMID: 24139800, DOI: 10.1016/j.celrep.2013.09.017.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAnimalsCarrier ProteinsCell Cycle ProteinsCell DifferentiationCells, CulturedEukaryotic Initiation FactorsMechanistic Target of Rapamycin Complex 1MiceMonomeric GTP-Binding ProteinsMultiprotein ComplexesNeural Stem CellsNeuropeptidesPhosphoproteinsPhosphorylationRas Homolog Enriched in Brain ProteinRibosomal Protein S6 Kinases, 90-kDaRNA InterferenceRNA, Small InterferingSirolimusTOR Serine-Threonine KinasesConceptsCap-dependent translationNeural stem cellsNSC differentiationStem Cell Self-RenewalTranslational repressor 4E-BP1P70 S6 kinase 1Neural Stem Cell Self-RenewalCell Self-RenewalRapamycin complex 1Neonatal neural stem cellsS6 kinase 1Downstream regulatory mechanismsLineage expansionSelf-RenewalRegulatory mechanismsKinase 1Kinase 1/2Constitutive activationMammalian targetCell growthStem cellsBrain sizeDifferentiationKnockdownNeuron production