2009
Ependymal cells along the lateral ventricle express functional P2X7 receptors
Genzen JR, Platel JC, Rubio ME, Bordey A. Ependymal cells along the lateral ventricle express functional P2X7 receptors. Purinergic Signalling 2009, 5: 299-307. PMID: 19274488, PMCID: PMC2717311, DOI: 10.1007/s11302-009-9143-5.Peer-Reviewed Original ResearchP2X7 receptorEpendymal cellsSubventricular zoneWhole-cell patch-clamp recordingsCentral nervous system injuryFunctional P2X7 receptorsFunctional purinergic receptorsNervous system injuryPatch-clamp recordingsMouse subventricular zoneSVZ neurogenic nicheNeural progenitor responseAbsence of responseSystem injuryCerebral ventricleProgenitor responseLateral ventricleNeurogenic nichePurinergic receptorsClamp recordingsExtracellular adenosineReceptorsCellular damageWidespread expressionInflammation
2004
Bergmann glial GlyT1 mediates glycine uptake and release in mouse cerebellar slices
Huang H, Barakat L, Wang D, Bordey A. Bergmann glial GlyT1 mediates glycine uptake and release in mouse cerebellar slices. The Journal Of Physiology 2004, 560: 721-736. PMID: 15331688, PMCID: PMC1665288, DOI: 10.1113/jphysiol.2004.067801.Peer-Reviewed Original ResearchConceptsWhole-cell patch-clamp recordingsGlycine receptor blockerMouse cerebellar slicesNMDA receptor activationVoltage-dependent inward currentsExtracellular glycine levelsPatch-clamp recordingsDependent glycine transportersReceptor blockersInhibitory neurotransmitterNMDA receptorsGlycine levelsBergmann gliaCerebellar slicesInward currentsReceptor activationGlycine transporterGliaGlycine transportGlyT1Glycine uptakeBlockersAgonistsIntact systemNeuronesGlial Glutamate Transporters Limit Spillover Activation of Presynaptic NMDA Receptors and Influence Synaptic Inhibition of Purkinje Neurons
Huang H, Bordey A. Glial Glutamate Transporters Limit Spillover Activation of Presynaptic NMDA Receptors and Influence Synaptic Inhibition of Purkinje Neurons. Journal Of Neuroscience 2004, 24: 5659-5669. PMID: 15215288, PMCID: PMC6729224, DOI: 10.1523/jneurosci.1338-04.2004.Peer-Reviewed Original ResearchMeSH KeywordsAction PotentialsAmino Acid Transport System X-AGAnimalsAntiportersCalciumCerebellumCystineEvoked PotentialsGamma-Aminobutyric AcidGlutamic AcidIn Vitro TechniquesMiceNerve FibersNeural InhibitionNeurogliaPatch-Clamp TechniquesPurkinje CellsReceptors, N-Methyl-D-AspartateReceptors, PresynapticRyanodineSynaptic TransmissionConceptsGlial glutamate transportersMiniature IPSCsPurkinje neuronsGlutamate transportersSpontaneous IPSCsSynaptic inhibitionInhibitory synapsesNMDAR activationWhole-cell patch-clamp recordingsPresynaptic NMDA receptorsPaired-pulse ratioMouse cerebellar slicesNMDA receptor activationPresynaptic NMDA receptor activationPatch-clamp recordingsBergmann glial cellsBeta-benzyloxyaspartic acidAdjacent Purkinje neuronsCystine-glutamate antiporterMIPSC frequencyGABAergic terminalsGABAergic synapsesGlial cellsNMDA receptorsExcitatory synapses
2002
GAT-1 and Reversible GABA Transport in Bergmann Glia in Slices
Barakat L, Bordey A. GAT-1 and Reversible GABA Transport in Bergmann Glia in Slices. Journal Of Neurophysiology 2002, 88: 1407-1419. PMID: 12205162, DOI: 10.1152/jn.2002.88.3.1407.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiological TransportCarrier ProteinsCerebellumElectrophysiologyGABA Plasma Membrane Transport ProteinsGamma-Aminobutyric AcidIn Vitro TechniquesMembrane ProteinsMembrane Transport ProteinsNeurogliaOrganic Anion TransportersPatch-Clamp TechniquesRatsRats, Sprague-DawleyReceptors, GABA-AConceptsGAT-1Bergmann gliaInward currentsGABA transporterWhole-cell patch-clamp recordingsCell patch-clamp recordingsGlial GABA uptakePatch-clamp recordingsRat cerebellar slicesGABA perfusionReceptor blockersAmbient GABANNC-711Extracellular GABAGABA effluxGABA uptakeGABA receptorsCerebellar slicesGAT subtypesNipecotic acidReceptor activationGABABlockersGliaOutward currentsCarrier‐mediated uptake and release of taurine from Bergmann glia in rat cerebellar slices
Barakat L, Wang D, Bordey A. Carrier‐mediated uptake and release of taurine from Bergmann glia in rat cerebellar slices. The Journal Of Physiology 2002, 541: 753-767. PMID: 12068038, PMCID: PMC2290349, DOI: 10.1113/jphysiol.2001.015834.Peer-Reviewed Original ResearchConceptsRat cerebellar slicesBergmann gliaGuanidinoethyl sulphonateCerebellar slicesTaurine transporterInward currentsWhole-cell patch-clamp recordingsTaurine uptakeGABA receptor blockersTaurine transporter inhibitorRelease of taurinePatch-clamp recordingsDependent taurine transporterOrder of potencyTaurine perfusionReceptor blockersIntracellular taurineIschemic conditionsCarrier-mediated uptakeTaurine effluxOutward currentsTransporter currentsTransporter inhibitorsIntracellular perfusionGlia
2001
Electrophysiological Characteristics of Reactive Astrocytes in Experimental Cortical Dysplasia
Bordey A, Lyons SA, Hablitz JJ, Sontheimer H. Electrophysiological Characteristics of Reactive Astrocytes in Experimental Cortical Dysplasia. Journal Of Neurophysiology 2001, 85: 1719-1731. PMID: 11287494, DOI: 10.1152/jn.2001.85.4.1719.Peer-Reviewed Original ResearchConceptsGap junction couplingReactive gliosisFreeze lesionGlial fibrillary acidic protein immunoreactivityWhole-cell patch-clamp recordingsCell patch-clamp recordingsExperimental cortical dysplasiaCortical layers IPatch-clamp recordingsPostnatal day 16Reduced gap junction couplingNormal neuronal functionDysplastic neocortexP24 ratsDysplastic cortexReactive astrocytesCortical dysplasiaNeuronal dysfunctionProtein immunoreactivityElectrophysiological characteristicsAstrocytesChannel expressionNeuronal functionMicrosulcusLayer I
1998
Properties of human glial cells associated with epileptic seizure foci
Bordey A, Sontheimer H. Properties of human glial cells associated with epileptic seizure foci. Epilepsy Research 1998, 32: 286-303. PMID: 9761328, DOI: 10.1016/s0920-1211(98)00059-x.Peer-Reviewed Original ResearchConceptsSeizure focusGlial cellsMesio-temporal lobe epilepsyWhole-cell patch-clamp recordingsLucifer YellowControl rat hippocampusPathophysiology of seizuresSlow action potentialsCurrent-clamp studiesGFAP-positive astrocytesEpileptic seizure fociPatch-clamp recordingsHuman glial cellsLobe epilepsyAcute slicesRat hippocampusEpilepsy patientsClamp studiesRat astrocytesAstrocytesIntermediate filaments GFAPNormal astrocytesAction potentialsPhysiological propertiesBranched processesElectrophysiological Properties of Human Astrocytic Tumor Cells In Situ: Enigma of Spiking Glial Cells
Bordey A, Sontheimer H. Electrophysiological Properties of Human Astrocytic Tumor Cells In Situ: Enigma of Spiking Glial Cells. Journal Of Neurophysiology 1998, 79: 2782-2793. PMID: 9582244, DOI: 10.1152/jn.1998.79.5.2782.Peer-Reviewed Original ResearchMeSH KeywordsAction PotentialsAstrocytesAstrocytomaBrain NeoplasmsCarcinomaCell DifferentiationChildChoroid Plexus NeoplasmsDelayed Rectifier Potassium ChannelsHumansIon TransportNeoplasm ProteinsNeoplastic Stem CellsNerve Tissue ProteinsPatch-Clamp TechniquesPotassiumPotassium Channel BlockersPotassium ChannelsPotassium Channels, Inwardly RectifyingPotassium Channels, Voltage-GatedSodium Channel BlockersSodium ChannelsSpinal CordTetraethylammoniumTetrodotoxinConceptsAstrocytoma cellsTumor cellsGlial cellsWhole-cell patch-clamp recordingsCell patch-clamp recordingsAction potential-like responsesOlder pediatric patientsSpinal cord astrocytesHuman astrocytic tumor cellsSensitive sodium currentsGroup of tumorsLow-grade astrocytomasPatch-clamp recordingsOutward potassium currentGlial tumor cellsAstrocytic tumor cellsGeneration of spikesHigh input resistancePeritumoral astrocytesPediatric patientsMicroM Ba2Pilocytic astrocytomaPotassium currentAstrocytesElectrophysiological propertiesExpression of voltage-activated chloride currents in acute slices of human gliomas
Ullrich N, Bordey A, Gillespie GY, Sontheimer H. Expression of voltage-activated chloride currents in acute slices of human gliomas. Neuroscience 1998, 83: 1161-1173. PMID: 9502255, DOI: 10.1016/s0306-4522(97)00456-9.Peer-Reviewed Original ResearchConceptsAcute slicesChloride currentsGlioma cellsGrade tumorsGlial cellsPatient biopsiesWhole-cell patch-clamp recordingsChloride channelsHost glial cellsImplanted glioma cellsPathological tumor gradeLow-grade tumorsHigh-grade tumorsPatch-clamp recordingsSpecific Cl- channelsNormal glial cellsAstrocyte-like cellsShort-term cultureTumor gradePathological gradeSCID miceVoltage-activated sodiumIntracranial injectionPotassium currentExperimental tumors
1997
Postnatal Development of Ionic Currents in Rat Hippocampal Astrocytes In Situ
Bordey A, Sontheimer H. Postnatal Development of Ionic Currents in Rat Hippocampal Astrocytes In Situ. Journal Of Neurophysiology 1997, 78: 461-477. PMID: 9242294, DOI: 10.1152/jn.1997.78.1.461.Peer-Reviewed Original ResearchConceptsHippocampal astrocytesPostnatal developmentCurrent sensitiveGlial cellsWhole-cell patch-clamp recordingsCell patch-clamp recordingsVoltage-activated sodium currentsGlial fibrillary acidic proteinRat hippocampal slicesRat hippocampal astrocytesFibrillary acidic proteinPatch-clamp recordingsOutward potassium currentEarly postnatal developmentIon channel expressionPercentage of cellsSteady-state activationStrong inward rectificationStratum radiatumHippocampal slicesCA1 regionDevelopmental changesPotassium currentChannel expressionEmbryonic rats