2023
4E-BP1 expression in embryonic postmitotic neurons mitigates mTORC1-induced cortical malformations and behavioral seizure severity but does not prevent epilepsy in mice
Nguyen L, Sharma M, Bordey A. 4E-BP1 expression in embryonic postmitotic neurons mitigates mTORC1-induced cortical malformations and behavioral seizure severity but does not prevent epilepsy in mice. Frontiers In Neuroscience 2023, 17: 1257056. PMID: 37680968, PMCID: PMC10480503, DOI: 10.3389/fnins.2023.1257056.Peer-Reviewed Original ResearchBehavioral seizure severityCortical malformationsSeizure frequencySeizure severityRadial gliaLayer 2/3 pyramidal neuronsCortical layer 2/3 pyramidal neuronsFocal cortical malformationsBehavioral seizuresNeuronal hypertrophyIntractable seizuresPyramidal neuronsSeizure activityRapamycin complex 1 (mTORC1) pathwayEctopic neuronsSeizure preventionMigratory neuronsWhite matterSeizuresNeuronsMalformationsMechanistic targetRecent evidenceSeverityEpilepsy
2016
Normalizing translation through 4E-BP prevents mTOR-driven cortical mislamination and ameliorates aberrant neuron integration
Lin TV, Hsieh L, Kimura T, Malone TJ, Bordey A. Normalizing translation through 4E-BP prevents mTOR-driven cortical mislamination and ameliorates aberrant neuron integration. Proceedings Of The National Academy Of Sciences Of The United States Of America 2016, 113: 11330-11335. PMID: 27647922, PMCID: PMC5056085, DOI: 10.1073/pnas.1605740113.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAnimalsCarrier ProteinsCell Cycle ProteinsDendritic SpinesEukaryotic Initiation FactorsExcitatory Postsynaptic PotentialsGene Knockdown TechniquesGreen Fluorescent ProteinsMatrix Attachment Region Binding ProteinsMechanistic Target of Rapamycin Complex 1MiceNeurogliaNeuronsPhosphoproteinsProtein BiosynthesisRas Homolog Enriched in Brain ProteinRNA CapsRNA, Small InterferingSignal TransductionTOR Serine-Threonine KinasesTranscription FactorsConceptsBrain cytoarchitectureUpper layer cortical neuronsHyperactive mammalian targetDendritic hypertrophyCortical neuronsCap-dependent translationEctopic placementRadial gliaMammalian targetLate corticogenesisTranslational repressor eukaryotic initiation factor 4EEukaryotic initiation factor 4ENeurodevelopmental disordersProtein 1Rapamycin complex 1Molecular hallmarksInitiation factor 4EMechanisms downstreamCytoarchitectureMolecular identityMisplacementActive mutantHypertrophyGliaOveractivation
2006
GFAP‐expressing cells in the postnatal subventricular zone display a unique glial phenotype intermediate between radial glia and astrocytes
Liu X, Bolteus AJ, Balkin DM, Henschel O, Bordey A. GFAP‐expressing cells in the postnatal subventricular zone display a unique glial phenotype intermediate between radial glia and astrocytes. Glia 2006, 54: 394-410. PMID: 16886203, DOI: 10.1002/glia.20392.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid Transport System X-AGAnimalsAnimals, NewbornAstrocytesBiomarkersCell DifferentiationCell ShapeConnexinsEpendymaGlial Fibrillary Acidic ProteinGlutamic AcidGreen Fluorescent ProteinsMembrane PotentialsMiceMice, TransgenicOrgan Culture TechniquesPatch-Clamp TechniquesPhenotypePotassiumPotassium ChannelsRecombinant Fusion ProteinsStem CellsTelencephalonConceptsGlial fibrillary acidic proteinPostnatal subventricular zoneSubventricular zoneGFAP-expressing cellsRadial gliaAstroglial marker glial fibrillary acidic proteinGlial propertiesEpendymal cellsGlutamate transportersGLT-1 glutamate transporterMarker glial fibrillary acidic proteinAMPA-type glutamate receptorsFunctional glutamate transportersFibrillary acidic proteinHuman glial fibrillary acidic proteinAdult subventricular zoneConnexin 43 expressionGap junction couplingNeural stem cellsMicroM Ba2Acute slicesAstrocytic functionsGlutamate receptorsGlial phenotypeClamp recordings