mTORC1 Targets the Translational Repressor 4E-BP2, but Not S6 Kinase 1/2, to Regulate Neural Stem Cell Self-Renewal In Vivo
Hartman NW, Lin TV, Zhang L, Paquelet GE, Feliciano DM, Bordey A. mTORC1 Targets the Translational Repressor 4E-BP2, but Not S6 Kinase 1/2, to Regulate Neural Stem Cell Self-Renewal In Vivo. Cell Reports 2013, 5: 433-444. PMID: 24139800, DOI: 10.1016/j.celrep.2013.09.017.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAnimalsCarrier ProteinsCell Cycle ProteinsCell DifferentiationCells, CulturedEukaryotic Initiation FactorsMechanistic Target of Rapamycin Complex 1MiceMonomeric GTP-Binding ProteinsMultiprotein ComplexesNeural Stem CellsNeuropeptidesPhosphoproteinsPhosphorylationRas Homolog Enriched in Brain ProteinRibosomal Protein S6 Kinases, 90-kDaRNA InterferenceRNA, Small InterferingSirolimusTOR Serine-Threonine KinasesConceptsCap-dependent translationNeural stem cellsNSC differentiationStem Cell Self-RenewalTranslational repressor 4E-BP1P70 S6 kinase 1Neural Stem Cell Self-RenewalCell Self-RenewalRapamycin complex 1Neonatal neural stem cellsS6 kinase 1Downstream regulatory mechanismsLineage expansionSelf-RenewalRegulatory mechanismsKinase 1Kinase 1/2Constitutive activationMammalian targetCell growthStem cellsBrain sizeDifferentiationKnockdownNeuron productionNeonatal subventricular zone electroporation.
Feliciano DM, Lafourcade CA, Bordey A. Neonatal subventricular zone electroporation. Journal Of Visualized Experiments 2013 PMID: 23426329, PMCID: PMC3601042, DOI: 10.3791/50197.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsNeural stem cellsGenetic engineeringEmbryonic neural stem cellsWhole animal levelMultiple cell typesSVZ neural stem cellsMammalian systemsMolecular pathwaysCell typesStem cellsTime-effective alternativeRodent forebrainAnimal levelElectroporationEpendymal cellsInvertebratesCellsCortical developmentRobust labelingProgenyCentral nervous system disordersNervous system disordersDifferentiationPathwayVast majority