2005
Assays for measuring extracellular GABA levels and cell migration rate in acute slices
Bolteus AJ, Garganta C, Bordey A. Assays for measuring extracellular GABA levels and cell migration rate in acute slices. Brain Research 2005, 14: 126-134. PMID: 15721818, DOI: 10.1016/j.brainresprot.2004.12.005.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiological AssayCell MovementGABA Plasma Membrane Transport ProteinsGamma-Aminobutyric AcidImmunohistochemistryLateral VentriclesMass SpectrometryMembrane Transport ModulatorsMembrane Transport ProteinsMiceMicroscopy, VideoNeurochemistryNeuronsOrgan Culture TechniquesReceptors, GABAStem CellsTelencephalonConceptsRostral migratory streamSubventricular zoneEndogenous GABAGABA levelsGABA receptorsNeuronal precursorsCell migrationLocal GABA levelsExtracellular GABA levelsAcute brain slicesAdult rodent brainPostnatal subventricular zoneFuture therapeutic useNeuronal precursor migrationAcute slicesBrain slicesNeurotransmitter GABASVZ cellsOlfactory bulbRodent brainRMS explantsMigratory streamTherapeutic useNeural precursorsGABA
2004
GABA Release and Uptake Regulate Neuronal Precursor Migration in the Postnatal Subventricular Zone
Bolteus AJ, Bordey A. GABA Release and Uptake Regulate Neuronal Precursor Migration in the Postnatal Subventricular Zone. Journal Of Neuroscience 2004, 24: 7623-7631. PMID: 15342728, PMCID: PMC6729616, DOI: 10.1523/jneurosci.1999-04.2004.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAstrocytesBicucullineBiological TransportBrainCalcium SignalingCell MovementGABA Plasma Membrane Transport ProteinsGABA-A Receptor AgonistsGamma-Aminobutyric AcidMass SpectrometryMembrane Transport ProteinsMiceMicroscopy, VideoNeuronsPatch-Clamp TechniquesPotassiumReceptors, GABA-AStem CellsConceptsAstrocyte-like cellsSubventricular zoneNeuronal precursorsPostnatal subventricular zoneNeuronal precursor migrationCell migrationPrecursor migrationPresence of bicucullineEnhancement of GABAAnterior subventricular zoneInhibition of GABARostral migratory streamAmbient GABA levelsApplication of GABASagittal brain slicesAntagonist bicucullineGABA releaseEndogenous GABAGABA levelsBrain slicesAdult miceGABA transporterMigratory streamGABACell depolarization
2003
Chemokine modulation of high‐conductance Ca2+‐sensitive K+ currents in microglia from human hippocampi
Bordey A, Spencer DD. Chemokine modulation of high‐conductance Ca2+‐sensitive K+ currents in microglia from human hippocampi. European Journal Of Neuroscience 2003, 18: 2893-2898. PMID: 14656339, DOI: 10.1111/j.1460-9568.2003.03021.x.Peer-Reviewed Original ResearchConceptsHigh-conductance Ca2MIP1-alphaAcute pathological processesNon-activated microgliaSensitive outward currentsPharmaco-resistant epilepsyPatch-clamp recordingsOutward current amplitudeHigh input resistanceChemokine modulationHippocampal slicesInjury siteBrain slicesChronic pathologiesMicrogliaEpileptic patientsAmeboid morphologyIntracellular Ca2Human hippocampusOutward currentsMigratory effectsInput resistancePositive labelingPathological processesMode of actionGABA Depolarizes Neuronal Progenitors of the Postnatal Subventricular Zone Via GABAA Receptor Activation
Wang DD, Krueger DD, Bordey A. GABA Depolarizes Neuronal Progenitors of the Postnatal Subventricular Zone Via GABAA Receptor Activation. The Journal Of Physiology 2003, 550: 785-800. PMID: 12807990, PMCID: PMC2343064, DOI: 10.1113/jphysiol.2003.042572.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAnimalsBarbituratesBenzodiazepinesCerebral VentriclesDose-Response Relationship, DrugElectrophysiologyGABA AntagonistsGamma-Aminobutyric AcidGlutamate DecarboxylaseIsoenzymesMembrane PotentialsMiceNeostriatumNeuronsPatch-Clamp TechniquesPicrotoxinPotassium ChannelsReceptors, GABA-AStem CellsZincConceptsRostral migratory streamGABAA receptor activationSubventricular zoneNeuronal progenitorsReceptor activationGlutamic acid decarboxylase 67Resting potentialsPostnatal mammalian brainPatch-clamp recordingsSVZ/RMSMouse brain slicesRecorded cellsCell-attached patchesGABA responsesBrain slicesNeurotransmitter GABASubset of progenitorsBenzodiazepine agonistsEmbryonic neuronsAction potentialsMammalian brainMigratory streamGABAInverse agonistSpecific receptorsBiophysical Properties and Ionic Signature of Neuronal Progenitors of the Postnatal Subventricular Zone In Situ
Wang DD, Krueger DD, Bordey A. Biophysical Properties and Ionic Signature of Neuronal Progenitors of the Postnatal Subventricular Zone In Situ. Journal Of Neurophysiology 2003, 90: 2291-2302. PMID: 12801891, DOI: 10.1152/jn.01116.2002.Peer-Reviewed Original ResearchConceptsRostral migratory streamSubventricular zonePassive membrane propertiesNeuronal progenitorsCell-attached patch-clamp recordingsNeuron-specific class IIIPostnatal mammalian brainPostnatal day 15Patch-clamp recordingsMigration of progenitorsPostnatal subventricular zoneHigh input resistanceCell-attached patchesBrain slicesOlfactory bulbMature neuronsStrong immunoreactivityTransient outwardDay 15KDR currentElectrophysiological propertiesAction potentialsMammalian brainMigratory streamClass IIIModulation of Glutamatergic Transmission by Bergmann Glial Cells in Rat Cerebellum In Situ
Bordey A, Sontheimer H. Modulation of Glutamatergic Transmission by Bergmann Glial Cells in Rat Cerebellum In Situ. Journal Of Neurophysiology 2003, 89: 979-988. PMID: 12574474, DOI: 10.1152/jn.00904.2002.Peer-Reviewed Original ResearchMeSH Keywords6-Cyano-7-nitroquinoxaline-2,3-dioneAmino Acid Transport System X-AGAnimalsAspartic AcidCell CommunicationElectric StimulationExcitatory Amino Acid AgonistsExcitatory Amino Acid AntagonistsExcitatory Postsynaptic PotentialsGlutamic AcidMembrane PotentialsNerve FibersNeurogliaN-MethylaspartatePatch-Clamp TechniquesPurkinje CellsRatsRats, Sprague-DawleyReceptors, GlutamateSodiumSynaptic TransmissionConceptsExcitatory postsynaptic currentsBergmann glial cellsGlial cellsPurkinje cell synapseInward currentsCell synapseGlu transportPurkinje cellsSpontaneous excitatory postsynaptic currentsAbsence of TTXPresynaptic glutamate releaseCerebellar brain slicesSpontaneous EPSC frequencyPatch-clamp recordingsSingle glial cellsIonotropic Glu receptorsParallel fibersEPSC frequencyPresynaptic effectsExcitatory transmissionGlutamate releaseGlutamatergic transmissionPostsynaptic currentsGlu receptorsBrain slices
2000
Ion channel expression by astrocytes in situ: Comparison of different CNS regions
Bordey A, Sontheimer H. Ion channel expression by astrocytes in situ: Comparison of different CNS regions. Glia 2000, 30: 27-38. PMID: 10696142, DOI: 10.1002/(sici)1098-1136(200003)30:1<27::aid-glia4>3.0.co;2-#.Peer-Reviewed Original ResearchConceptsBergmann glial cellsIon channel complementGlial cellsIon channel expressionDifferent CNS regionsCNS regionsChannel expressionPercentage of astrocytesTransient A-typeCerebellar Bergmann glial cellsChannel complementPatch-clamp recordingsVoltage-activated currentsPostnatal day 17Whole-cell currentsIon channelsVoltage-gated ion channelsBrain slicesRat astrocytesAstrocytesDay 17Low input resistanceInput resistanceCNSInitial recording
1999
Differential Inhibition of Glial K+ Currents by 4-AP
Bordey A, Sontheimer H. Differential Inhibition of Glial K+ Currents by 4-AP. Journal Of Neurophysiology 1999, 82: 3476-3487. PMID: 10601476, DOI: 10.1152/jn.1999.82.6.3476.Peer-Reviewed Original ResearchMeSH Keywords4-AminopyridineAction PotentialsAnimalsAstrocytesCells, CulturedDelayed Rectifier Potassium ChannelsElectrophysiologyLarge-Conductance Calcium-Activated Potassium ChannelsNeurogliaPatch-Clamp TechniquesPotassium Channel BlockersPotassium ChannelsPotassium Channels, Calcium-ActivatedPotassium Channels, Inwardly RectifyingPotassium Channels, Voltage-GatedRatsRats, Sprague-DawleySpinal CordTetraethylammoniumConceptsAcute spinal cord slicesSpinal cord slicesConcentration-dependent blockPotassium channel typesType currentsDose-dependent inhibitorNeuronal action potentialsCord slicesSpinal cordChannel blockersEpileptiform dischargesBrain slicesAction potentialsHomeostatic functionsA-currentAstrocytesDifferential inhibitionRepolarizationMembrane potentialChannel typesSlicesBlockersGlialCord