2015
Minimalist Model Systems Reveal Similarities and Differences between Membrane Interaction Modes of MCL1 and BAK*
Landeta O, Landajuela A, Garcia-Saez A, Basañez G. Minimalist Model Systems Reveal Similarities and Differences between Membrane Interaction Modes of MCL1 and BAK*. Journal Of Biological Chemistry 2015, 290: 17004-17019. PMID: 25987560, PMCID: PMC4505444, DOI: 10.1074/jbc.m114.602193.Peer-Reviewed Original ResearchConceptsBCL2 family proteinsFamily proteinsStructural foldMembrane environmentStable heterodimeric complexMembrane association modesFluorescence cross-correlation spectroscopyBCL2 family membersMembrane interaction modesCross-correlation spectroscopyCell fateMembrane associationHeterodimeric complexBCL2 familyMitochondrial membraneCellular factorsFluorescence-based techniquesMode of actionApoptosis-related factorsBakKey modulatorFRET measurementsMCL1ProteinCardiolipin content
2013
Proapoptotic Bax and Bak Proteins Form Stable Protein-permeable Pores of Tunable Size
Bleicken S, Landeta O, Landajuela A, Basañez G, García-Sáez A. Proapoptotic Bax and Bak Proteins Form Stable Protein-permeable Pores of Tunable Size. Journal Of Biological Chemistry 2013, 288: 33241-33252. PMID: 24100034, PMCID: PMC3829170, DOI: 10.1074/jbc.m113.512087.Peer-Reviewed Original ResearchConceptsMitochondrial outer membraneApoptotic poreOuter membraneMitochondrial apoptotic factorsSingle vesicle levelActivity of BaxProapoptotic protein BaxProteinaceous channelsVesicle levelMembrane permeabilizationBak proteinForm poresProtein BaxProapoptotic BaxCardiolipin concentrationCytochrome cApoptotic factorsBaxBakPermeabilizationProteinSimilar mechanismMembraneMembrane poresProtein concentration