In vitro evolution of high-titer, virus-like vesicles containing a single structural protein
Rose NF, Buonocore L, Schell JB, Chattopadhyay A, Bahl K, Liu X, Rose JK. In vitro evolution of high-titer, virus-like vesicles containing a single structural protein. Proceedings Of The National Academy Of Sciences Of The United States Of America 2014, 111: 16866-16871. PMID: 25385608, PMCID: PMC4250146, DOI: 10.1073/pnas.1414991111.Peer-Reviewed Original ResearchMeSH KeywordsAlphavirusAmino Acid MotifsBiological EvolutionIn Vitro TechniquesMicroscopy, Electron, TransmissionViral Structural ProteinsConceptsVirus-like vesiclesStructural proteinsCapsid proteinCell surfaceLate domain motifsVesicular stomatitis virus glycoproteinVSV G proteinPlasmid-based systemOnly structural proteinSingle structural proteinDomain motifReplicase proteinsReplication factoriesMultiple point mutationsExtensive passagingG proteinsRNA repliconsRNA virusesPoint mutationsProteinEfficient packagingInnate immunityMutationsVirus glycoproteinVesicles