Expansion of human mesenchymal stem/stromal cells (hMSCs) in bioreactors using microcarriers: lessons learnt and what the future holds
Couto P, Rotondi M, Bersenev A, Hewitt C, Nienow A, Verter F, Rafiq Q. Expansion of human mesenchymal stem/stromal cells (hMSCs) in bioreactors using microcarriers: lessons learnt and what the future holds. Biotechnology Advances 2020, 45: 107636. PMID: 32980437, DOI: 10.1016/j.biotechadv.2020.107636.Peer-Reviewed Original ResearchConceptsHuman mesenchymal stem/stromal cellsGene therapy applicationsManufacturing processMesenchymal stem/stromal cellsStem/stromal cellsCurrent engineering challengesTherapy applicationsImproved process monitoringMulti-differentiation capabilitiesProcess efficiencyEngineering challengesMicrocarriersProcess monitoringProcess variabilityBioreactorHuman operationProduction costsPotential future areasGood consistencyCartilage repairFinal productAffordable priceCellular interventionsRelevant scalesCell quality