2016
Design and synthesis of purine analogues as highly specific ligands for FcyB, a ubiquitous fungal nucleobase transporter
Lougiakis N, Gavriil E, Kairis M, Sioupouli G, Lambrinidis G, Benaki D, Krypotou E, Mikros E, Marakos P, Pouli N, Diallinas G. Design and synthesis of purine analogues as highly specific ligands for FcyB, a ubiquitous fungal nucleobase transporter. Bioorganic & Medicinal Chemistry 2016, 24: 5941-5952. PMID: 27720327, DOI: 10.1016/j.bmc.2016.09.055.Peer-Reviewed Original ResearchMeSH KeywordsAspergillus nidulansDose-Response Relationship, DrugDrug DesignLigandsMolecular Docking SimulationMolecular StructureNucleobase Transport ProteinsPurinesStructure-Activity Relationship
2015
Functional characterization of NAT/NCS2 proteins of Aspergillus brasiliensis reveals a genuine xanthine–uric acid transporter and an intrinsically misfolded polypeptide
Krypotou E, Scazzocchio C, Diallinas G. Functional characterization of NAT/NCS2 proteins of Aspergillus brasiliensis reveals a genuine xanthine–uric acid transporter and an intrinsically misfolded polypeptide. Fungal Genetics And Biology 2015, 75: 56-63. PMID: 25639910, DOI: 10.1016/j.fgb.2015.01.009.Peer-Reviewed Original ResearchConceptsNucleobase-ascorbate transporterUptake of purinesAcid transportAspergillus nidulansMisfolded polypeptidesER-retainedGFP tagMisfolded proteinsHeterologous expressionEvolutionary implicationsInactive proteinPutative transportersAspergillus brasiliensisFunctional characterizationTurned-overPlasma membranePeptide sequencesIn silicoProteinSubfamilyAspergillusTransport functionLow affinityHigher affinityVacuoles
2014
Modelling, substrate docking and mutational analysis identify residues essential for function and specificity of the major fungal purine transporter AzgA
Krypotou E, Lambrinidis G, Evangelidis T, Mikros E, Diallinas G. Modelling, substrate docking and mutational analysis identify residues essential for function and specificity of the major fungal purine transporter AzgA. Molecular Microbiology 2014, 93: 129-145. PMID: 24818808, DOI: 10.1111/mmi.12646.Peer-Reviewed Original ResearchConceptsSubstrate dockingMutation analysisNucleobase-ascorbate transporterAmino acid residuesNAT familyAspergillus nidulansInverted repeatsSubstrate bindingSubstrate translocationA-helicesPurine bindingC-tailAcid residuesTransporter familyCytoplasmic N-H(+) bindingAzgATMS8TMS10Founding memberFlexible domainsBinding cavityGate domainTMS3Residues