2018
META RNA profiling: Multiplexed quantitation of targeted RNAs across large numbers of samples
Narayan A, Johnkennedy R, Zakaria M, Lee V, Patel AA. META RNA profiling: Multiplexed quantitation of targeted RNAs across large numbers of samples. Methods 2018, 152: 41-47. PMID: 30308315, PMCID: PMC6311137, DOI: 10.1016/j.ymeth.2018.09.012.Peer-Reviewed Original Research
2002
The splicing of U12‐type introns can be a rate‐limiting step in gene expression
Patel AA, McCarthy M, Steitz JA. The splicing of U12‐type introns can be a rate‐limiting step in gene expression. The EMBO Journal 2002, 21: 3804-3815. PMID: 12110592, PMCID: PMC126102, DOI: 10.1093/emboj/cdf297.Peer-Reviewed Original ResearchConceptsU12-type intronsGene expressionDrosophila melanogaster S2 cellsProtein-coding genesU12-type spliceosomePost-transcriptional regulationHuman tissue culture cellsU2-type intronsMetazoan genomesTissue culture cellsS2 cellsU12-typeIntron removalIdentical mRNAIntronsFluorescent proteinQuantitative RT-PCR assayMinigene constructsCulture cellsRate-limiting stepSpliceosomeMRNAMinor classExpressionRT-PCR assays