Research Resources

Transcript

• 00:00Thanks again, Linda.
• 00:02So just to say there's three of us at
• 00:06the at the four of us actually more
• 00:08than that at the leadership level.
• 00:10But the key people to know aside
• 00:13from myself is John Crystal on
• 00:16the grant that supports the,
• 00:18we call it the what's called the Clinical
• 00:21Translational Science Award from NIH.
• 00:23I'll just mention this in a minute.
• 00:25John Crystal,
• 00:26who's also Chair of Psychiatry and
• 00:29myself are the KOPI eyes on that grant.
• 00:31So we're obviously very deeply involved
• 00:34and Dave Coleman has come in as an
• 00:37Interim Director for for YCCI as well,
• 00:40which has really been great because Dave
• 00:44really is devoting all of his time.
• 00:45Dave was faculty here for for a number
• 00:49of years and then became a chair of
• 00:51medicine up at Boston University.
• 00:53And when he retired,
• 00:54somehow Nancy convinced him that what
• 00:56he wanted to do was to have another
• 00:58job that took 180% of his time up.
• 01:00I'm not sure how she did it, but it's great.
• 01:03So just to say so the Yale Center
• 01:06for Clinical Investigation is the
• 01:08broad infra and superstructure,
• 01:10if you will, for clinical and
• 01:12translational research here at Yale.
• 01:14That doesn't mean it operates all by itself.
• 01:16The Cancer Center has a lot of,
• 01:18a lot of its work.
• 01:19The departments have a lot of work within
• 01:21the departments in these areas and so forth.
• 01:23And it basically has sort
• 01:25of two funding mechanisms.
• 01:26Just so you're aware,
• 01:28there's the underlying grant,
• 01:30which is we were actually in the
• 01:33first group that received this
• 01:35major grant from from the NIH.
• 01:37That's whatever it is now,
• 01:38must be 1617 years ago, 18 years ago.
• 01:42I'm trying not to remember because
• 01:44I know we have to renew again in two
• 01:46years and and in addition to that
• 01:49there's somewhere between 20 and $25 million.
• 01:51It's actually contributed not just
• 01:53by the Dean's office,
• 01:54but to its credit by the owner haven
• 01:56health system to support clinical
• 01:58and translational research.
• 01:59And I think that's a a good example of
• 02:01kind of the partnership that I think is,
• 02:03is continuing to grow and and
• 02:06get better and better over time.
• 02:08We do a variety of things and let
• 02:10me just move on to that within YCCI
• 02:12that you guys should know about.
• 02:13And I want to make sure I leave
• 02:15enough time for Amy because she's a
• 02:17lot of substantive information to
• 02:18talk about on both based on basic
• 02:20cores and what have you as well.
• 02:21So what the YCCI supports is
• 02:23certainly educational programs for
• 02:25junior faculty members as well as
• 02:27a variety of trainees,
• 02:28students and very significant
• 02:31part at for training staff.
• 02:33One of the issues for those of you
• 02:35who are engaged in clinical and
• 02:37translational research frankly is
• 02:39workforce that's been challenging
• 02:40particularly since Kovat a lot of
• 02:42people work remotely now which is fine,
• 02:45but the total workforce that's
• 02:47out there sort of diminished.
• 02:48And so the fact that we can
• 02:51train up everybody
• 02:52is really good in terms of
• 02:54keeping that that workflow going.
• 02:55We also do pilot funding for in
• 02:58a variety of targeted areas that
• 03:00would that changes every year
• 03:02and there are some core research
• 03:04resource facilities that we support.
• 03:06And I'll tell you more about
• 03:07all this in just a minute.
• 03:09This past safe.
• 03:10This past safe staff and research
• 03:13support services that go along with this.
• 03:15One of the things that again is,
• 03:17is something that is crucial if
• 03:19you're doing a lot of this kind of
• 03:21research is all the regulatory issues
• 03:23INDIDE's you know all the stuff that
• 03:26goes along with that and and that's
• 03:28within the responsibility and the and
• 03:30the the the daily work of the Yale
• 03:34Center for Clinical Investigation.
• 03:35And just to be clear, and again,
• 03:36I think this reflects well on the
• 03:39university and I think it's why we,
• 03:40why where we are, we excel.
• 03:43I think it's part of the reason that we
• 03:45excel and that is that we really don't
• 03:47think of this is just a School of Medicine,
• 03:49a grant.
• 03:49And in fact it is a grant
• 03:50to the whole university.
• 03:51And there's huge participation not
• 03:52just by the School of Medicine,
• 03:54but by the School of Public Health,
• 03:55School of Nursing,
• 03:57actually the engineering school,
• 03:58the Faculty of Arts and Sciences
• 04:00and even even the law school and the
• 04:02School of Management have particular
• 04:03parts that we help to support
• 04:05through the YCCI for various types
• 04:07of really I think kind of exciting
• 04:09clinical and translational research.
• 04:11So this is just some of the educational
• 04:13funding opportunities and again,
• 04:15not worth going and into any
• 04:17great details on this,
• 04:18but just to say there's a
• 04:21multidisciplinary predoctoral program,
• 04:22there's a multidisciplinary
• 04:24postdoctoral program.
• 04:25Again,
• 04:25things that we can talk about and
• 04:27you can find out more about as
• 04:29needed if you're if you're you
• 04:31know hiring on grad students,
• 04:33postdocs etcetera.
• 04:33There's also two of the major
• 04:36educational programs that actually Yale
• 04:37number of years ago was instrumental
• 04:39kind of across the country and
• 04:41creating one is called the National
• 04:44Clinical Clinician Scholars Program.
• 04:46That's actually what some of you may
• 04:48know is the old Robert Wood Johnson
• 04:50program and that is a master's
• 04:52degree granting program with a very,
• 04:55very rigorous curriculum and
• 04:56really has is a two year long
• 05:01educational experience that
• 05:02really is, is excellent.
• 05:04And then the investigative
• 05:05medicine program is one that you
• 05:07may or may not be familiar with.
• 05:08This generally is for individuals who
• 05:10are MD Onlys who want to obtain a pH.
• 05:13D degree so that they can do their
• 05:15investigative work in that context.
• 05:16And that's exactly what this program does.
• 05:19And so if you are,
• 05:21if you have just the just the MD degree,
• 05:25many of us kind of do that and
• 05:26managed to do research on that.
• 05:28But it's not a bad thing to necessarily get
• 05:30the training for a PhD degree on top of it.
• 05:32And that can either be in the
• 05:34kind of in the more of the health
• 05:36services side of research and those
• 05:38kinds of things or even very,
• 05:39very basic, you know,
• 05:40flies and fish and all those kinds
• 05:42of wonderful things research.
• 05:43There's a number of other things
• 05:45on the faculty level.
• 05:46There's the YCCI Scholar Awards
• 05:49and that again is a mechanism for
• 05:51for junior faculty and again maybe
• 05:53something you're interested in and
• 05:54we can talk about it separately
• 05:56not to take the time now.
• 05:57And that has been a very successful program.
• 05:59It's really great.
• 06:00Of the folks that have come through it,
• 06:02we're always proud of this,
• 06:0398% are still engaged in research.
• 06:05There's been about 1000 grants
• 06:06that those folks have managed to
• 06:09to produce over the last 15 years
• 06:12or so with about almost a billion
• 06:14dollars worth of research funding
• 06:15from that and lots of publication.
• 06:17So it works well.
• 06:18It does extend across the
• 06:19entire research spectrum,
• 06:21again from very basic mechanisms
• 06:22all the way up to to public
• 06:25health types of types of issues.
• 06:28On the second thing here that we provide,
• 06:29there's pilot funding and core resources.
• 06:31The pilots are announced once
• 06:33a year that won't be coming out
• 06:35until the late fall this year.
• 06:38We also support some of the core resources,
• 06:41for example, the pets,
• 06:42a couple of the imaging centers,
• 06:44the PET Research Center and the Mr.
• 06:46Magnetic Residence Research Center,
• 06:49the Biostatistics and the bioinformatics
• 06:52support mechanisms for for clinical
• 06:55and translational research and
• 06:57actually help to also support the Yale
• 07:00Center for Genomic Analysis as well.
• 07:04So again that's kind of takes care of
• 07:06the first two things that the YCCI does.
• 07:09Then there's kind of the space and staff,
• 07:11and I managed to say it right that
• 07:13time and research support services,
• 07:15there's basically 4 area space areas that
• 07:18are out there, just so you're aware.
• 07:20And again, this is all the kind,
• 07:22there's a very high level kind of view.
• 07:24Just so you know, for outpatient work.
• 07:26There's a thing called the Church St.
• 07:27South Research Unit, which is,
• 07:31which is nice.
• 07:32It actually has a very patient friendly
• 07:34parking which is not always true in
• 07:36New Haven and has the ability to see
• 07:39patients there for research protocols,
• 07:40even do infusions provide for the
• 07:44bottoming support and so forth and so
• 07:46on. There's an inpatient
• 07:47unit which is called the HRU,
• 07:49the human research unit within the
• 07:51confines of Yellow Haven Hospital.
• 07:53There's actually a sort of a new two
• 07:55bed unit that's right where the imaging
• 07:57centers are because sometimes people
• 07:59really need that aspect of things,
• 08:01but you need to do an infusion
• 08:03or this or that beforehand and
• 08:05support and that that helps to
• 08:07increase throughput for for clinical
• 08:09trials and translational trials.
• 08:11And we actually have a unit out of
• 08:13what's called the West Campus which
• 08:14was designed for COVID for individuals
• 08:17who were who were COVID infected and
• 08:19who were going to get trials and we're
• 08:21getting trials for that group of patients.
• 08:23So we can keep it isolated and
• 08:25all that's still out there and
• 08:26is is another effective unit.
• 08:29I'm not going to talk details
• 08:30about workforce development.
• 08:31I kind of alluded to that a few moments ago.
• 08:34But again that's one of the ways
• 08:35we have to try to keep enough
• 08:37support out there for the kinds of
• 08:40individuals that you need to do that,
• 08:42this kind of this kind of research.
• 08:45There is,
• 08:45there is a part of the YCCI
• 08:48that helps with recruitment.
• 08:50It extends all the way from something
• 08:52called the help us Discover clinical
• 08:53research awareness campaign,
• 08:54which actually includes
• 08:58samples and a number of individuals
• 09:00who have said they're fast.
• 09:03You know, they're they're very
• 09:04happy to be involved as sort of
• 09:06normal controls and what have you,
• 09:07all of which is very helpful.
• 09:09And there's a whole kind of
• 09:10infrastructure to try to,
• 09:11to try to do that. Right now,
• 09:13most of what we do is centered at
• 09:16Young Haven Hospital and this medical,
• 09:18the academic Medical Center here
• 09:20and the Yale School of Medicine.
• 09:21We do extend out to some of the
• 09:24other hospitals that Bobber was
• 09:26talking about earlier today.
• 09:27We have a goal of the course of
• 09:29next two years to make that part
• 09:31of doing the research that we can
• 09:33do truly across the entire health
• 09:35system smoother for everybody.
• 09:36One of the things that's been
• 09:39particularly successful particularly
• 09:40in in trying to get over all the very,
• 09:43very significant historical barriers
• 09:45to a lot of communities being
• 09:47involved in clinical research
• 09:49and not trusting our enterprise.
• 09:50We've really done a terrible
• 09:52job convincing people out there
• 09:54it doesn't matter who that that
• 09:57we're actually trying to do help
• 09:59instead of harm to the populations.
• 10:01Nevertheless,
• 10:02this was created now about 15 years,
• 10:0410 years ago, 11 years ago.
• 10:06The culture ambassador collaboration
• 10:08works through a variety of community
• 10:11groups including the AM E Zion
• 10:12churches and some of the pastors and
• 10:15and and individuals in in the in the
• 10:17church as well as one of the advocacy
• 10:21groups for for Hispanic individuals,
• 10:23Latinx individuals across the state.
• 10:25And that's been very successful and
• 10:27something else that's available.
• 10:28It's a really,
• 10:29truly a bidirectional kind of
• 10:31operation which is really nice.
• 10:32I mean they tell us what they
• 10:34think we should be doing,
• 10:35we tell them what we're doing and we
• 10:37try to get together on the on those
• 10:39two things and that's been terrific.
• 10:40There's a lot of compliance issues out there.
• 10:42Again,
• 10:42that's kind of the whole regulatory part.
• 10:44I won't torture you with all of that,
• 10:46but we have an infrastructure that tries
• 10:48to meet a lot of the things that become real,
• 10:52real issues for for these
• 10:55kinds of investigations.
• 10:57Finally,
• 10:59just to mention again that this is
• 11:01focused not just at the School of
• 11:02Medicine but across the the health
• 11:05system and across the various other
• 11:08schools within within Yale University.
• 11:11This is kind of a geographic view of
• 11:14all the places that that the YCCI touches.
• 11:17Many of these places have places
• 11:19to do pure research operations.
• 11:22Sometimes that's a coordinated with
• 11:24our clinical sites out there through
• 11:26Yale Medicine and Yellow Haven Health System.
• 11:28We want to do more and more
• 11:30of that coordination.
• 11:31Some of them are freestanding sites
• 11:34meeting particular needs that people have
• 11:36for their for their clinical research.
• 11:39We do actually interact well with
• 11:40the with the VA on these kinds
• 11:42of things as best one can with
• 11:44a federal entity which can be
• 11:45challenging in and of itself.
• 11:47There are a variety of other community
• 11:49partners out there,
• 11:51including the two FQHC's in in New Haven,
• 11:55Fair Haven and Cornell Scott.
• 11:57And there's actually a fairly
• 11:59good rapport with the community
• 12:00practices that may practice it.
• 12:02Young Haven Hospital and
• 12:05across the health system.
• 12:07There is a website.
• 12:10Gee, we're surprised and there's a
• 12:12variety of ways to kind of use that
• 12:14to help navigate your way around.
• 12:16But again, questions and things like that,
• 12:18please feel free to to,
• 12:20to e-mail myself, Dave Coleman,
• 12:23John, what have you that then that
• 12:26would be that would be great.
• 12:28And you can find all this on the website.
• 12:30You can actually do it by just kind
• 12:32of Googling YCCI plus whatever
• 12:34it is you're looking for and
• 12:35that that pretty much works well
• 12:39form which is short read sequencing.
• 12:41But we also have a microarray core which
• 12:44does long read sequencing with the pack bio.
• 12:47The microarray core also does 10X genomics.
• 12:49So we really do a lot of single cell work
• 12:52and we're actually going to be expanding
• 12:55on those on those types of services.
• 12:58And then both YCGA and microarray
• 13:01do offer spatial transcriptomex type
• 13:03services which we are working on
• 13:05expanding those and bringing some new
• 13:08technologies in for both of these cores.
• 13:10The staff provide services for
• 13:12next generation sequencing,
• 13:13bioinformatics and microarrays.
• 13:17We on top of the next generation sequencing
• 13:19we do offer a Sanger sequencing core.
• 13:21So this is a separate core and they do
• 13:24anything from individual samples to plates,
• 13:26PCR purification.
• 13:27They have an extensive a list of
• 13:30fragment analysis services and
• 13:31this is all staff provided.
• 13:33So you would drop samples off and you will
• 13:35get data you know usually within 8 hours.
• 13:37We really work to have a very
• 13:39quick turnaround time on that.
• 13:41Same thing kind of goes for
• 13:42the oligo resource.
• 13:43So we'll do anything from you know
• 13:45your standard primers to really
• 13:47complex oligo so you can reach out to
• 13:49them and they will help you design,
• 13:52you know trimmer base like there's
• 13:53there's just so much that they're
• 13:55doing at this point,
• 13:56so you can reach out to them and they
• 13:58will help you with some custom synthesis.
• 14:02Our biophysics resource studies oligomeric
• 14:04states of biomolecular assemblies.
• 14:07So there's several technologies available,
• 14:08light scattering surface plasma
• 14:12and resonance, you know,
• 14:13field flow fractionation and we
• 14:16recently added a nanoparticle tracking
• 14:18analysis technology to this course.
• 14:20So that has proven to be quite useful
• 14:23and you know it's it's an exciting
• 14:26addition to the biophysics resource.
• 14:28Our macromolecular structure analysis
• 14:30consultation core doesn't actually
• 14:32run any samples but what we have
• 14:34in this core is expertise.
• 14:36So if you have any macromolecular
• 14:39structures or cryoem based images
• 14:41that you really need some help
• 14:43with the analysis of it.
• 14:45So Janine is wonderful and he you
• 14:48know can really help answer the
• 14:50questions that you were asking in the
• 14:521st place and really push a lot of
• 14:54these structures to publication our
• 14:56mass spec and proteomics facility.
• 14:58They do everything from basic protein
• 15:00ID to intact protein determination.
• 15:03They do high resolution for
• 15:05small molecule work,
• 15:06targeted proteomics and they
• 15:09do some metabolomics services.
• 15:12Everything is staff provided though
• 15:14they do have one Open Access
• 15:16instrument so that you would,
• 15:18you know with some help from the staff
• 15:20you would independently be able to
• 15:22design your experiment and you know go
• 15:24through everything up to data collection.
• 15:27One of our newest cores is
• 15:28the chemical metabolism core.
• 15:30This is all staff provided services,
• 15:32but they specialize in mitochondrial
• 15:35work and metabolomics,
• 15:37so you can definitely reach out to them.
• 15:39They do like I'm a MOSA platform that's
• 15:43again not available anywhere but Yale,
• 15:45so it's very specialized.
• 15:49The Yale Genome Editing Center creates
• 15:52genetically engineered mice via
• 15:54CRISPR. They do cryopreservation.
• 15:56They also do assisted
• 15:58reproduction techniques.
• 16:00This is all staff provided
• 16:02services and they're they're
• 16:03very much into working with you
• 16:06to design exactly what you need.
• 16:08And they have,
• 16:09they can really tailor their
• 16:10services based on your needs.
• 16:14Our zebrafish phenotyping core they do,
• 16:17they create transgenic zebrafish and then
• 16:19they provide assistance in phenotyping.
• 16:22This is all staff provided
• 16:24service and again very,
• 16:26very tailored to what you know your needs
• 16:30are the Yale Center for Molecular Discovery.
• 16:32So they have resources for assay
• 16:34development and high throughput
• 16:35screening mostly for drug discovery.
• 16:37So this core is very specialized in the
• 16:39fact that you know all of your all of
• 16:42your experiments would be very different.
• 16:43So they definitely work with you from
• 16:45the beginning to design the best you know
• 16:48technique for what you're looking to do.
• 16:50This is all staff provided service,
• 16:52so they do have a self-service laboratory.
• 16:54So once you're trained on the instruments,
• 16:55you would have independent independent use.
• 17:00And we have a stem cell core,
• 17:02they do I PSC derivation,
• 17:04genomic editing,
• 17:05pluripotent stem cell quality testing.
• 17:08They have really a full range of stem
• 17:12cell technologies available and they they
• 17:15routinely kind of change around whatever
• 17:17they're doing in order to meet the needs.
• 17:20And they they really specialize in some
• 17:22of the extremely hard genomic editing.
• 17:25They're they're able to really do
• 17:27a lot of things that other places
• 17:29just aren't capable of and these are
• 17:32all staff provided services also.
• 17:34And finally, we have our custom machine shop.
• 17:37So a lot of times if you can't
• 17:39find something for your lab,
• 17:40you can reach out to Tony and he
• 17:42will actually fabricate, you know,
• 17:44design and fabricate something
• 17:45completely custom to to meet your needs.
• 17:48So like I said,
• 17:49it's a very unique resource to have
• 17:51on campus and very, very valuable.
• 17:55So please reach out to me with any
• 17:57questions at any time and then this
• 17:59can be found on the OAPD website.
• 18:01So you can read through all the
• 18:03information that I did not discuss
• 18:06today and I keep this updated and
• 18:07you can reach out to me,
• 18:09you can reach out to Brian,
• 18:10you can reach out to any of the
• 18:12cores at any time.
• 18:13And we really look forward to
• 18:15working with you.