2016
Statistical modeling and removal of lipid membrane projections for cryo-EM structure determination of reconstituted membrane proteins
Jensen KH, Brandt SS, Shigematsu H, Sigworth FJ. Statistical modeling and removal of lipid membrane projections for cryo-EM structure determination of reconstituted membrane proteins. Journal Of Structural Biology 2016, 194: 49-60. PMID: 26835990, PMCID: PMC4866491, DOI: 10.1016/j.jsb.2016.01.012.Peer-Reviewed Original Research
2013
Quantifying the local resolution of cryo-EM density maps
Kucukelbir A, Sigworth FJ, Tagare HD. Quantifying the local resolution of cryo-EM density maps. Nature Methods 2013, 11: 63-65. PMID: 24213166, PMCID: PMC3903095, DOI: 10.1038/nmeth.2727.Peer-Reviewed Original Research
2009
Structure of the BK potassium channel in a lipid membrane from electron cryomicroscopy
Wang L, Sigworth FJ. Structure of the BK potassium channel in a lipid membrane from electron cryomicroscopy. Nature 2009, 461: 292-295. PMID: 19718020, PMCID: PMC2797367, DOI: 10.1038/nature08291.Peer-Reviewed Original ResearchConceptsVoltage sensor domainNative membrane environmentMembrane proteinsMembrane environmentChannel voltage-sensor domainElectron cryomicroscopyVoltage-activated potassium channelsSingle-particle electron cryomicroscopyIndividual protein particlesPotassium channelsBK potassium channelsLarge conductance calciumLipid membranesX-ray crystal structureBK channelsStructural studiesProtein particlesProteinCryomicroscopyCrystal structureFormation of crystalsDomainStructureCrystalsChannels
2006
Using cryo-EM to measure the dipole potential of a lipid membrane
Wang L, Bose PS, Sigworth FJ. Using cryo-EM to measure the dipole potential of a lipid membrane. Proceedings Of The National Academy Of Sciences Of The United States Of America 2006, 103: 18528-18533. PMID: 17116859, PMCID: PMC1693696, DOI: 10.1073/pnas.0608714103.Peer-Reviewed Original ResearchMeSH KeywordsCryoelectron MicroscopyLipid BilayersLiposomesMembrane PotentialsOsmotic PressurePhosphatidylcholinesConceptsDipole potentialLipid membranesPhase-contrast imagingMolecular dynamics calculationsLipid bilayer membranesIon translocation ratesAtomic potentialLipid moleculesLipid monolayersBilayer membranesDynamics calculationsProbe methodSurface potentialAnionsDiphytanoylphosphatidylcholineCationsElectronsImage intensityAbsolute valueMembraneMoleculesConformationLarge permeabilityMonolayersBilayers
1998
A Maximum-Likelihood Approach to Single-Particle Image Refinement
Sigworth F. A Maximum-Likelihood Approach to Single-Particle Image Refinement. Journal Of Structural Biology 1998, 122: 328-339. PMID: 9774537, DOI: 10.1006/jsbi.1998.4014.Peer-Reviewed Original ResearchMeSH KeywordsCryoelectron MicroscopyImage Processing, Computer-AssistedLikelihood FunctionsMacromolecular SubstancesMolecular StructureParticle Size