2023
An optogenetic-phosphoproteomic study reveals dynamic Akt1 signaling profiles in endothelial cells
Zhou W, Li W, Wang S, Salovska B, Hu Z, Tao B, Di Y, Punyamurtula U, Turk B, Sessa W, Liu Y. An optogenetic-phosphoproteomic study reveals dynamic Akt1 signaling profiles in endothelial cells. Nature Communications 2023, 14: 3803. PMID: 37365174, PMCID: PMC10293293, DOI: 10.1038/s41467-023-39514-1.Peer-Reviewed Original ResearchMeSH KeywordsEndothelial CellsHumansOptogeneticsPhosphorylationProtein Serine-Threonine KinasesProto-Oncogene Proteins c-aktSignal TransductionConceptsPhosphorylation sitesSerine/threonine kinase AktMass spectrometry-based phosphoproteomicsThreonine kinase AktAkt-dependent phosphorylationAberrant Akt activationEndothelial cellsKinase substrateKinase AktCell signalingPhosphorylation profilePhenotypic outcomesDownstream signalingAkt activationAkt1 phosphorylationHuman diseasesSystem-level analysisAKT1Vascular endothelial cellsRich resourcePhosphorylationSignalingGrowth factorAktCells
2020
BMP-9 and LDL crosstalk regulates ALK-1 endocytosis and LDL transcytosis in endothelial cells
Tao B, Kraehling JR, Ghaffari S, Ramirez C, Lee S, Fowler JW, Lee WL, Fernandez-Hernando C, Eichmann A, Sessa WC. BMP-9 and LDL crosstalk regulates ALK-1 endocytosis and LDL transcytosis in endothelial cells. Journal Of Biological Chemistry 2020, 295: 18179-18188. PMID: 33097593, PMCID: PMC7939458, DOI: 10.1074/jbc.ra120.015680.Peer-Reviewed Original ResearchConceptsBone morphogenetic protein 9Dynamin 2Caveolin-1LDL transcytosisGene expressionActivin-like kinase 1Endothelial cellsEndothelial cell quiescenceCell surface levelsSmad1/5 phosphorylationExtensive endocytosisUnbiased screenProtein 9Cell quiescenceKinase 1ALK-1BMP-9 levelsEssential roleEndocytosisEndothelial homeostasisHeavy chainSignalingInternalizationTranscytosisCells