TuKiet Lam, PhD, BS
Cards
Appointments
Titles
Director, Keck MS & Proteomics Resource, Molecular Biophysics and Biochemistry; Director, Discovery Proteomics Core of Yale/NIDA Neuroproteomics Center, Molecular Biophysics and Biochemistry
Contact Info
Yale School of Medicine
Keck MS & Proteomics Resource, 300 George Street
New Haven, CT 06510
United States
Appointments
Titles
Director, Keck MS & Proteomics Resource, Molecular Biophysics and Biochemistry; Director, Discovery Proteomics Core of Yale/NIDA Neuroproteomics Center, Molecular Biophysics and Biochemistry
Contact Info
Yale School of Medicine
Keck MS & Proteomics Resource, 300 George Street
New Haven, CT 06510
United States
Appointments
Titles
Director, Keck MS & Proteomics Resource, Molecular Biophysics and Biochemistry; Director, Discovery Proteomics Core of Yale/NIDA Neuroproteomics Center, Molecular Biophysics and Biochemistry
Contact Info
Yale School of Medicine
Keck MS & Proteomics Resource, 300 George Street
New Haven, CT 06510
United States
About
Titles
Research Scientist
Director, Keck MS & Proteomics Resource, Molecular Biophysics and Biochemistry; Director, Discovery Proteomics Core of Yale/NIDA Neuroproteomics Center, Molecular Biophysics and BiochemistryBiography
TuKiet T. Lam (Tu) earned his undergraduate degree from Rollins College (Winter Park, FL) in 1998. He completed his Ph.D. training at Florida State University in 2002 with mentorship from Alan G. Marshall, Ph.D. After a brief postdoctoral training at the National High Magnetic Field Laboratory (Tallahassee, FL), he joined and headed the FT-ICR Users Facility and worked with Mark R. Emmett Ph.D. there until summer of 2004. He then joined Yale in fall of 2004 as Director to the FT-ICR MS Resource where he utilized his training and expertise in high resolution mass spectrometry to establish his collaborative career in providing state-of-the-art mass spectrometry instrumentation and analyses to investigators from a multitude of disciplines (e.g., Biological, chemical, clinical, pharmacological, environmental, etc.). He currently serves as the Director of the Keck MS & Proteomics Resource at the Yale School of Medicine.
Appointments
Molecular Biophysics and Biochemistry
Research ScientistPrimary
Other Departments & Organizations
Education & Training
- Postdoctoral Fellow
- National High Magnetic Field Laboratory (2003)
- PhD
- Florida State University, Chemistry/Analytical (2002)
- BS
- Rollins College, Chemistry (1998)
Research
Overview
I am the Director of the Keck MS & Proteomics Resource at the Yale School of Medicine and have been with Yale since 2004. My specific aims are to train, assist, collaborate, and make available my expertise in mass spectrometry to as many investigators of other scientific and biomedical discipline as possible so that they can advance their research. By establishing a well-organized and well-trained core group of staffs who are dedicated to the same philosophy and mission, my team provides the high-quality mass spectrometry data and analyses which have resulted in co-authorships and acknowledgements in many high impact scientific publications. My mass spectrometry (MS) footing grew from my graduate career at Florida State University, where I developed a hydrogen/deuterium exchange workflow to robustly observe protein-protein interaction(s) and structural changes in proteins. The technique led to the discovery of unique conformational changes in HIV-1 viral capsid protein (Lanman, et. al., JMB, 325, 759-772, 2003, Cover). I became involved in many collaborative projects during my postdoctoral work at the National High Magnetic Field Lab and began to shape my career in using my analytical chemistry skillsets in high resolution tandem mass spectrometry to work with a multitude of investigators across many basic scientific and applied biomedical disciplines to understand biological functions at the molecular level. My successful multidisciplinary collaborative interaction with these investigators falls in the areas of protein identification, profiling, and quantitation; protein posttranslational modification (PTM); protein-protein interactions; natural products; metabolites; green chemistry; and organic product syntheses among many others. During my residence within the Keck MS and Proteomics Resource, I collaborated many biomedical and clinical investigators in their aims to better understand protein(s) functions in various relevant disease and cancers studies such as illustrated in Fogel et al, 2010, (which was featured on the cover of J. Biol. Chem. 285, 34864); Musante et al, 2017 (Elife 6); Miller et al, 2017 (ACS Chem Neurosci, 8(7), 1554); Sun et al, 2016 (Arthritis Rheumatology, 68(5), 1252); Tellez et al, 2013 (Sciene 341(6147), 800); Klein et al, 2017 (Neruon 95, p281); Goffredo et al, 2017 (Nutrients, 9, p 642); and Henderson et al, 2016 (Acta Neuropathol 131, p621). Currently I am involved in many projects which utilizes high resolution mass spectrometry (Orbitrap family of mass spectrometers) Label Free Quantitative LC MS and LC MS/MS methodology for protein ID/profiling and quantitation in various biological systems (i.e. brain, serum, plasma, liver, endothelial cells, FACS sorted cells, neurons, worms, mosquitos, flies, etc.), for protein ubiquitinylation, palmitoylation, acetylation, and phosphorylation modification, for small molecule quantitation and structural elucidation along with targeted drug compounds assays. The use of these exceedingly more reproducible high resolution mass spectrometry to facilitate protein and protein posttranslational modification ID and quantitative global profiling provides a very robust and biostatistical confident way to monitor protein changes, functions, and interactions within a complex biological system.
Medical Subject Headings (MeSH)
Research at a Glance
Yale Co-Authors
Publications Timeline
Research Interests
Angus Nairn, PhD
Rolando Garcia Milian, MLS, AHIP
Ala F Nassar, PhD
Georgia Charkoftaki, PharmD, PhD
Kenneth Williams, PhD
Marina Picciotto, PhD
Proteomics
Mass Spectrometry
Tandem Mass Spectrometry
Proteins
Spectrometry, Mass, Electrospray Ionization
Chromatography, High Pressure Liquid
Publications
2024
Phosphorylation of the nuclear poly(A) binding protein (PABPN1) during mitosis protects mRNA from hyperadenylation and maintains transcriptome dynamics
Gordon J, Phizicky D, Schärfen L, Brown C, Escayola D, Kanyo J, Lam T, Simon M, Neugebauer K. Phosphorylation of the nuclear poly(A) binding protein (PABPN1) during mitosis protects mRNA from hyperadenylation and maintains transcriptome dynamics. Nucleic Acids Research 2024, gkae562. PMID: 38943343, DOI: 10.1093/nar/gkae562.Peer-Reviewed Original ResearchAltmetricConceptsPoly(A)-binding proteinTranscriptome dynamicsNuclear poly(A) binding proteinPoly(A) binding proteinMode of gene regulationFunctional consequences of phosphorylationLong-read sequencingIncreased mRNA turnoverNucleo-cytoplasmic exportConsequences of phosphorylationRegulation of poly(ACohort of mRNAsGene expression programsMRNA biogenesisCytoplasmic mixingMRNA turnoverGene regulationShorter poly(ARNA stabilityMitotic kinasesPoly(ACell cycleMRNA synthesisIncreased transcriptionBinding proteinBERNN: Enhancing classification of Liquid Chromatography Mass Spectrometry data with batch effect removal neural networks
Pelletier S, Leclercq M, Roux-Dalvai F, de Geus M, Leslie S, Wang W, Lam T, Nairn A, Arnold S, Carlyle B, Precioso F, Droit A. BERNN: Enhancing classification of Liquid Chromatography Mass Spectrometry data with batch effect removal neural networks. Nature Communications 2024, 15: 3777. PMID: 38710683, PMCID: PMC11074280, DOI: 10.1038/s41467-024-48177-5.Peer-Reviewed Original ResearchAltmetricMeSH Keywords and ConceptsConceptsLC-MS experimentsLC-MSLiquid chromatography mass spectrometry dataComplex biological samplesMass spectrometry dataLiquid chromatography mass spectrometryChromatography mass spectrometryMass spectrometrySpectrometry dataEffective removalBiological samplesExperimental conditionsBatch effect removalSample processing protocolBatch effectsSpectrometryBatch effect correction methodsCorrecting batch effectsRemoval of batch effectsOptimal conditions for carrying out trypsin digestions on complex proteomes: From bulk samples to single cells
Mansuri M, Bathla S, Lam T, Nairn A, Williams K. Optimal conditions for carrying out trypsin digestions on complex proteomes: From bulk samples to single cells. Journal Of Proteomics 2024, 297: 105109. PMID: 38325732, PMCID: PMC10939724, DOI: 10.1016/j.jprot.2024.105109.Peer-Reviewed Original ResearchCitationsAltmetricConceptsComplex proteomesProtein cleavage activityOptimal conditionsTrypsin digestion protocolReversed phase HPLC separationMass spectrometry workflowMS-based proteomicsMass spectrometric analysisC-terminal amino acid residuesTrypsin digestionChromatographic separationDigestion protocolAmino acid residuesHPLC separationMS/MS analysisGlobal proteomic analysisSingle cellsSample matrixSpectrometric analysisCleavage specificityGeneration of peptidesAcid residuesDown proteinsProteomic analysisCleavage activity
2023
Dual regulation of SLC25A39 by AFG3L2 and iron controls mitochondrial glutathione homeostasis
Shi X, DeCiucis M, Grabinska K, Kanyo J, Liu A, Lam T, Shen H. Dual regulation of SLC25A39 by AFG3L2 and iron controls mitochondrial glutathione homeostasis. Molecular Cell 2023, 84: 802-810.e6. PMID: 38157846, PMCID: PMC10922821, DOI: 10.1016/j.molcel.2023.12.008.Peer-Reviewed Original ResearchCitationsAltmetricConceptsProtein quality controlMitochondrial protein quality controlDual regulationMetabolic compartmentalizationIron homeostasisMitochondrial iron-sulfur clustersIron-sulfur clustersMitochondrial transportersProtein regulationMammalian cellsCRISPR knockoutCysteine residuesTransporter regulationLoop 1SLC25A39Glutathione homeostasisMetabolic sensingGlutathione uptakeMature neuronsProtein levelsHomeostasisRegulationAFG3L2Biochemical featuresMitochondrial glutathione levelsMass spectrometry on cerebrospinal fluid uncovers association of novel glycolysis biomarkers with Alzheimer’s disease in a complex clinical cohort
de Geus M, Leslie S, Wang W, Lam T, Broekman M, Neefjes S, Nairn A, Carlyle B, Arnold S. Mass spectrometry on cerebrospinal fluid uncovers association of novel glycolysis biomarkers with Alzheimer’s disease in a complex clinical cohort. Alzheimer's & Dementia 2023, 19 DOI: 10.1002/alz.078466.Peer-Reviewed Original ResearchConceptsAlzheimer's diseaseMild cognitive impairmentHospital neurology serviceNon-AD diagnosesDiagnostic lumbar puncturePrognosis of ADCerebrospinal fluid biomarkersClinic cohortFurther mechanistic studiesNeurology serviceFDG-PETBrain metabolismFluid biomarkersLumbar puncturePatient cohortClinical indicationsDisease progressionATN biomarkersClinical cohortTreatment responseComplex cohortBackground DiagnosisCSF samplesTreatment efficacyLinear regression analysisMicrobiome Depletion Increases Fentanyl Self-Administration and Alters the Striatal Proteome Through Short-Chain Fatty Acids
Hofford R, Meckel K, Wiser E, Wang W, Sens J, Kim M, Godino A, Lam T, Kiraly D. Microbiome Depletion Increases Fentanyl Self-Administration and Alters the Striatal Proteome Through Short-Chain Fatty Acids. ENeuro 2023, 11: eneuro.0388-23.2023. PMID: 38164564, PMCID: PMC10875718, DOI: 10.1523/eneuro.0388-23.2023.Peer-Reviewed Original ResearchCitationsAltmetricMass spectrometry in cerebrospinal fluid uncovers association of glycolysis biomarkers with Alzheimer’s disease in a large clinical sample
de Geus M, Leslie S, Lam T, Wang W, Roux-Dalvai F, Droit A, Kivisakk P, Nairn A, Arnold S, Carlyle B. Mass spectrometry in cerebrospinal fluid uncovers association of glycolysis biomarkers with Alzheimer’s disease in a large clinical sample. Scientific Reports 2023, 13: 22406. PMID: 38104170, PMCID: PMC10725469, DOI: 10.1038/s41598-023-49440-3.Peer-Reviewed Original ResearchCitationsAltmetricCommon Pathways of Epileptogenesis in Patients With Epilepsy Post–Brain Injury
Misra S, Khan E, Lam T, Mazumder R, Gururangan K, Hickman L, Goswami V, Funaro M, Eldem E, Sansing L, Sico J, Quinn T, Liebeskind D, Montaner J, Kwan P, Mishra N. Common Pathways of Epileptogenesis in Patients With Epilepsy Post–Brain Injury. Neurology 2023, 101: e2243-e2256. PMID: 37550071, PMCID: PMC10727219, DOI: 10.1212/wnl.0000000000207749.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsStandardized mean differencePoststroke epilepsyBrain injuryGenetic susceptibilityDisparate time pointsRisk of epileptogenesisLate-onset seizuresDevelopment of epilepsyBlood glucose levelsTraumatic brain injuryMean biomarker levelsIndividual genetic susceptibilityWeb of ScienceReported biomarkersPrimary outcomeEpileptogenic processBiofluid biomarkersBias assessmentBiomarker levelsCommon biological pathwaysEnrichment analysisGlucose levelsHigh riskPrognostic studiesEpilepsyNatural isoaspartyl protein modification of ZAP70 alters T cell responses in lupus
Yang M, Lam T, Kanyo J, Kang I, Zhou Z, Clarke S, Mamula M. Natural isoaspartyl protein modification of ZAP70 alters T cell responses in lupus. Autoimmunity 2023, 56: 2282945. PMID: 37994408, PMCID: PMC10897934, DOI: 10.1080/08916934.2023.2282945.Peer-Reviewed Original ResearchAltmetricMulti-omics profiling reveals cellular pathways and functions regulated by ALDH1B1 in colon cancer cells
Wang Y, Popovic Z, Charkoftaki G, Garcia-Milian R, Lam T, Thompson D, Chen Y, Vasiliou V. Multi-omics profiling reveals cellular pathways and functions regulated by ALDH1B1 in colon cancer cells. Chemico-Biological Interactions 2023, 384: 110714. PMID: 37716420, PMCID: PMC10807983, DOI: 10.1016/j.cbi.2023.110714.Peer-Reviewed Original ResearchConceptsColon cancer cellsCellular stress response pathwaysStress response pathwaysMulti-omics analysisCancer cellsSecond messenger signalingMulti-omics profilingNew molecular informationFunctional annotationCellular functionsResponse pathwaysKinase signalingCellular pathwaysColon adenocarcinoma cell lineHuman colon adenocarcinoma cell lineApoptosis signalingEnrichment analysisAldehyde dehydrogenase 1B1Molecular signaturesAdenocarcinoma cell lineMolecular informationSignalingNovel targetProtein expressionCell lines
Academic Achievements and Community Involvement
activity Reviewer
CommitteesNHMFL - ICR Users ProgramDetails07/20/2012 - PresentDescriptionNHMFL ICR User Proposal Review Committee Members (External)activity Member
Professional OrganizationsAmerican Society for Mass SpectrometryDetails2000 - Presentactivity Member
Professional OrganizationsAmerican Chemical SocietyDetails04/01/1999 - Presentactivity Keck MS & Proteomics Resource
Oral PresentationInternational Post Stroke Epilepsy Research ConsortiumDetails12/02/2023 - 12/02/2023Orlando, FL, United States
Links & Media
Media
Proteomics Workflow
Basic Bottom-Up proteomics workflow for protein identification, profiling, and quantitation.
News
- July 16, 2020Source: American Journal of Physiology-Gastrointestinal and Liver Physiology
Glucocorticoids and serum- and glucocorticoid-inducible kinase 1 are potent regulators of CFTR in the native intestine: implications for stress-induced diarrhea
- August 06, 2014
In search for Alzheimer’s drug, a major STEP forward
- August 15, 2013
Messenger between gut and brain linked to eating behavior
Related Links
Get In Touch
Contacts
Yale School of Medicine
Keck MS & Proteomics Resource, 300 George Street
New Haven, CT 06510
United States
Locations
Keck MS & Proteomics Resource
Academic Office
300 George Street, Fl Ground, Ste G005
New Haven, CT 06511
Appointments
203.785.5086Keck MS & Proteomics Resource
Lab
300 George Street, Fl Ground, Ste G001
New Haven, CT 06511
Appointments
203.737.2205