2000
Expression of GABAC receptor ρ1 and ρ2 subunits during development of the mouse retina
Greka A, Lipton S, Zhang D. Expression of GABAC receptor ρ1 and ρ2 subunits during development of the mouse retina. European Journal Of Neuroscience 2000, 12: 3575-3582. PMID: 11029627, DOI: 10.1046/j.1460-9568.2000.00247.x.Peer-Reviewed Original ResearchConceptsRetinal developmentGamma-aminobutyric acid type C receptorMouse retinaRho subunitsReceptor-mediated currentsPostnatal day 6Type C receptorsBipolar cell differentiationMouse retinal developmentΡ2 subunitsBipolar cellsC receptorRho2 subunitsRho1 subunitDay 6ReceptorsEye openingSubunit transcriptionSubunit messageAdulthoodRetinaFuture studiesCompetitive PCRCell differentiationP9Evidence for coassembly of mutant GABACρ1 with GABAAγ2S, glycine α1 and glycine α2 receptor subunits in vitro
Pan Z, Zhang D, Zhang X, Lipton S. Evidence for coassembly of mutant GABACρ1 with GABAAγ2S, glycine α1 and glycine α2 receptor subunits in vitro. European Journal Of Neuroscience 2000, 12: 3137-3145. PMID: 10998097, DOI: 10.1046/j.1460-9568.2000.00198.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsDose-Response Relationship, DrugElectrophysiologyGABA AntagonistsGamma-Aminobutyric AcidGene ExpressionIn Vitro TechniquesMutagenesisNeural InhibitionOocytesPicrotoxinProtein Structure, TertiaryRatsReceptors, GABAReceptors, GABA-AReceptors, GABA-BReceptors, GlycineRetinaXenopus laevisConceptsGlycine alpha1Rho1 subunitAlpha2 subunitHeteromeric receptorsGABA dose-response curveBlockade of GABAVoltage-clamp recordingsGamma-aminobutyric acidXenopus laevis oocyte expression systemTwo-electrode voltage-clamp recordingsDose-response curveResponse propertiesPicrotoxinin sensitivityOocyte expression systemGamma2 subunitGABAReceptor subunitsPharmacological propertiesReceptorsUnique gatingFunctional evidenceGABAAAlpha1Gamma2Homomeric
1997
Agonist-induced closure of constitutively open γ-aminobutyric acid channels with mutated M2 domains
Pan Z, Zhang D, Zhang X, Lipton S. Agonist-induced closure of constitutively open γ-aminobutyric acid channels with mutated M2 domains. Proceedings Of The National Academy Of Sciences Of The United States Of America 1997, 94: 6490-6495. PMID: 9177245, PMCID: PMC21077, DOI: 10.1073/pnas.94.12.6490.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCloning, MolecularFemaleGABA AgonistsGABA AntagonistsGamma-Aminobutyric AcidIon Channel GatingIon ChannelsMacromolecular SubstancesMembrane PotentialsMolecular Sequence DataMutagenesis, Site-DirectedOocytesOrganophosphorus CompoundsPoint MutationRatsReceptors, GABAReceptors, GlycineReceptors, NicotinicRecombinant ProteinsSequence AlignmentSequence Homology, Amino AcidVirulence Factors, BordetellaXenopus laevisConceptsLigand-gated ion channelsChannel gatingIon channelsAmino acid residuesAminobutyric acid channelsSingle-point mutantsAbsence of ligandChannel pore regionStructure-function relationshipsPoint mutantsAcid channelImportant new insightsAllosteric transitionSubunit resultsAcid residuesM2 domainConstitutive currentReceptor proteinPore regionAgonist bindingAgonist regulationSubstituting alanineM2 regionSpontaneous channel openingChannel opening
1995
Multiple GABA receptor subtypes mediate inhibition of calcium influx at rat retinal bipolar cell terminals
Pan Z, Lipton. Multiple GABA receptor subtypes mediate inhibition of calcium influx at rat retinal bipolar cell terminals. Journal Of Neuroscience 1995, 15: 2668-2679. PMID: 7722621, PMCID: PMC6577770, DOI: 10.1523/jneurosci.15-04-02668.1995.Peer-Reviewed Original ResearchConceptsBipolar cell terminalsGABA receptor subtypesGABA receptorsRetinal bipolar cell terminalsGABAC receptorsGABAA receptorsReceptor subtypesCell terminalsMultiple GABA receptorsDihydropyridine-sensitive Ca2Pharmacologic profileCalcium imaging methodSynaptic transmissionCalcium influxSlow onsetInhibitory effectReceptorsSubtypesReversible increaseAgonistsGABACa2FA kineticsFunctional roleInhibitionDifferential modulation by sulfhydryl redox agents and glutathione of GABA- and glycine-evoked currents in rat retinal ganglion cells
Pan Z, Bahring R, Grantyn R, Lipton. Differential modulation by sulfhydryl redox agents and glutathione of GABA- and glycine-evoked currents in rat retinal ganglion cells. Journal Of Neuroscience 1995, 15: 1384-1391. PMID: 7869105, PMCID: PMC6577822, DOI: 10.1523/jneurosci.15-02-01384.1995.Peer-Reviewed Original ResearchConceptsRetinal ganglion cellsRat retinal ganglion cellsGlycine-evoked currentsGanglion cellsInhibitory neurotransmissionSingle retinal ganglion cellsInhibitory neurotransmitter systemsWhole-cell recordingsInhibitory receptor systemsModulatory systemAction of glutathioneNeurotransmitter systemsReceptor stimulationMammalian CNSMammalian retinaSingle agentEndogenous redox agentsReceptor systemPatch pipetteGABADifferential modulationGABAANeurotransmissionRetinaExtracellular glutathione
1992
Desensitization of GABA-activated currents and channels in cultured cortical neurons
Frosch M, Lipton, Dichter M. Desensitization of GABA-activated currents and channels in cultured cortical neurons. Journal Of Neuroscience 1992, 12: 3042-3053. PMID: 1379634, PMCID: PMC6575652, DOI: 10.1523/jneurosci.12-08-03042.1992.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCerebral CortexDose-Response Relationship, DrugElectrophysiologyGamma-Aminobutyric AcidIon ChannelsNeuronsTime FactorsConceptsRate of desensitizationPresence of agonistInhibitory synaptic activationCultured cortical neuronsWhole-cell recording modeApplication of GABAWhole-cell modeDesensitization of GABACortical inhibitionCortical neuronsGABA responsesNeocortical neuronsSynaptic activationSame neuronsGABASingle-channel responsesDesensitizationDesensitization processNeuronsTrue declineAgonistsMembrane conductanceCell culturesRecording modeDose dependence
1989
GABA-activated single channel currents in outside-out membrane patches from rat retinal ganglion cells
Lipton S. GABA-activated single channel currents in outside-out membrane patches from rat retinal ganglion cells. Visual Neuroscience 1989, 3: 275-279. PMID: 2487107, DOI: 10.1017/s0952523800010026.Peer-Reviewed Original ResearchConceptsRetinal ganglion cellsGamma-aminobutyric acidGanglion cellsWhole-cell currentsMammalian retinal ganglion cellsRat retinal ganglion cellsLarge whole-cell currentsMammalian central nervous systemWhole-cell recordingsCentral nervous systemPatch-clamp methodInhibitory transmitterWhole-cell responsesNervous systemCell-free patchesNervous tissueGABAA channelsRapid rundownDesensitization kineticsSingle-channel recordingsChannel currentsSingle-channel currentsExcised patch configurationsSingle-channel eventsUnitary conductance
1988
Characterization of GABA- and glycine-induced currents of solitary rodent retinal ganglion cells in culture
Tauck D, Frosch M, Lipton S. Characterization of GABA- and glycine-induced currents of solitary rodent retinal ganglion cells in culture. Neuroscience 1988, 27: 193-203. PMID: 3200439, DOI: 10.1016/0306-4522(88)90230-8.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBicucullineCells, CulturedGamma-Aminobutyric AcidGlycineMembrane PotentialsMiceMice, Inbred C57BLPicrotoxinRatsRetinaRetinal Ganglion CellsStrychnineTaurineConceptsGanglion cellsBicuculline methiodideRodent retinal ganglion cellsReversal potentialRetinal ganglion cellsWhole-cell recordingsAgonist-induced currentsRodent retinaD-tubocurarineMicroM GABAMicroM glycineReceptor desensitizationGABAPatch electrodeDesensitizationStrychnineMembrane conductancePicrotoxininMethiodideTaurineCellsSolitary cellsSpace clampTissue cultureResponse