2021
Soluble α-synuclein–antibody complexes activate the NLRP3 inflammasome in hiPSC-derived microglia
Trudler D, Nazor K, Eisele Y, Grabauskas T, Dolatabadi N, Parker J, Sultan A, Zhong Z, Goodwin M, Levites Y, Golde T, Kelly J, Sierks M, Schork N, Karin M, Ambasudhan R, Lipton S. Soluble α-synuclein–antibody complexes activate the NLRP3 inflammasome in hiPSC-derived microglia. Proceedings Of The National Academy Of Sciences Of The United States Of America 2021, 118: e2025847118. PMID: 33833060, PMCID: PMC8054017, DOI: 10.1073/pnas.2025847118.Peer-Reviewed Original ResearchConceptsHuman microgliaLike receptor family pyrinFibrillar αSynA9 dopaminergic neuronsInterleukin-1β secretionCaspase-1 activationMicroglial activationFamily pyrinAntibody therapyNeuronal deathParkinson's diseaseMicrogliaMouse brainΑ-synucleinDual stimulationMitochondrial damageΑSynAntibodiesInflammationNLRP3Cognate antibodiesHuman brainDiseaseNeuronsStem cells
2009
Molecular stages of rapid and uniform neuralization of human embryonic stem cells
Bajpai R, Coppola G, Kaul M, Talantova M, Cimadamore F, Nilbratt M, Geschwind D, Lipton S, Terskikh A. Molecular stages of rapid and uniform neuralization of human embryonic stem cells. Cell Death & Differentiation 2009, 16: 807-825. PMID: 19282867, PMCID: PMC3432273, DOI: 10.1038/cdd.2009.18.Peer-Reviewed Original ResearchConceptsHuman embryonic stem cellsEmbryonic stem cellsNeural precursor cellsDynamic gene expression analysisStem cellsGene expression changesPrecursor cellsGene expression analysisEarly human developmentCoregulated genesPrimitive ectodermExpression analysisExpression changesMouse brainPotent oncogeneMolecular signalingUniform differentiationHuman biologyEfficient differentiationFuture cell-based therapiesFunctional neuronsNeuralizationPosterior markersNeonatal mouse brainNeural proliferation
2000
Antiapoptotic role of the p38 mitogen-activated protein kinase–myocyte enhancer factor 2 transcription factor pathway during neuronal differentiation
Okamoto S, Krainc D, Sherman K, Lipton S. Antiapoptotic role of the p38 mitogen-activated protein kinase–myocyte enhancer factor 2 transcription factor pathway during neuronal differentiation. Proceedings Of The National Academy Of Sciences Of The United States Of America 2000, 97: 7561-7566. PMID: 10852968, PMCID: PMC16585, DOI: 10.1073/pnas.130502697.Peer-Reviewed Original ResearchConceptsMyocyte enhancer factor 2Mitogen-activated protein kinase p38alphaNeuronal differentiationDominant-negative p38alphaProtein kinase p38alphaDominant-negative formTranscription factor pathwaysMADS familyMEF2 familyMEF2 pathwayCell divisionTranscription factorsMyogenic phenotypeExpression patternsMyogenic factorsAntiapoptotic roleCell deathMammalian cerebral cortexP38alphaApoptotic deathNegative formPrecursor cellsFactor 2Factor pathwayApoptosis
1999
Absence of binding activity of neuron-restrictive silencer factor is necessary, but not sufficient for transcription of NMDA receptor subunit type 1 in neuronal cells
Okamoto S, Sherman K, Lipton S. Absence of binding activity of neuron-restrictive silencer factor is necessary, but not sufficient for transcription of NMDA receptor subunit type 1 in neuronal cells. Brain Research 1999, 74: 44-54. PMID: 10640675, DOI: 10.1016/s0169-328x(99)00250-8.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceCell DifferentiationDown-RegulationGene Expression RegulationHeLa CellsHumansLuciferasesMutationNeuronsPromoter Regions, GeneticProtein BindingReceptors, N-Methyl-D-AspartateRecombinant Fusion ProteinsRepressor ProteinsResponse ElementsRNA, MessengerSequence Homology, Nucleic AcidTranscription FactorsTranscription, GeneticTumor Cells, CulturedConceptsNRSF/RESTNeuron-restrictive silencer factorPromoter activityNR1 geneSilencer factorCell linesNRSE/RE1Set of genesNeuronal cellsType I geneNonneuronal cell linesREST proteinNeuronal cell lineI geneP19 cellsConsensus sequenceNeuronal differentiationGenesHeLa cellsTranscriptionNonneuronal cellsIndependent mannerNeuronal specificityMRNA levelsExpression
1993
Expression of endogenous NMDAR1 transcripts without receptor protein suggests post-transcriptional control in PC12 cells.
Sucher N, Brose N, Deitcher D, Awobuluyi M, Gasic G, Bading H, Cepko C, Greenberg M, Jahn R, Heinemann S, Lipton S. Expression of endogenous NMDAR1 transcripts without receptor protein suggests post-transcriptional control in PC12 cells. Journal Of Biological Chemistry 1993, 268: 22299-22304. PMID: 8226739, DOI: 10.1016/s0021-9258(18)41528-1.Peer-Reviewed Original ResearchMeSH KeywordsAlternative SplicingAnimalsAstrocytesBase SequenceCell DifferentiationCell LineCells, CulturedDimethylphenylpiperazinium IodideDNA PrimersGene ExpressionGene Expression Regulation, NeoplasticGenetic VariationHippocampusHumansKidneyMacromolecular SubstancesMembrane PotentialsMolecular Sequence DataMolecular WeightNerve Growth FactorsN-MethylaspartatePC12 CellsPolymerase Chain ReactionReceptors, N-Methyl-D-AspartateRNA, MessengerSynaptic MembranesTranscription, GeneticTransfectionConceptsNMDAR1 proteinPC12 cellsPost-transcriptional controlPost-transcriptional mechanismsNative PC12 cellsParticular cell typeUndifferentiated rat pheochromocytoma (PC12) cellsExpression of RNAPC12 cell lineTranslational regulationIsoform CRat pheochromocytoma cellsNorthern hybridizationExpression vectorReceptor proteinCell typesIon channelsProteinFunctional NMDACell linesPheochromocytoma cellsCytomegalovirus promoterModel systemRNAExpression