2022
Cutting Edge: mTORC2 Regulates CD8+ Effector and Memory T Cell Differentiation through Serum and Glucocorticoid Kinase 1.
Patel C, Heikamp E, Xu W, Sun I, Oh M, Sun I, Wen J, Tam A, Blosser R, Powell J. Cutting Edge: mTORC2 Regulates CD8+ Effector and Memory T Cell Differentiation through Serum and Glucocorticoid Kinase 1. The Journal Of Immunology 2022, 209: 2287-2291. PMID: 36469844, PMCID: PMC10065985, DOI: 10.4049/jimmunol.2100669.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCD8-Positive T-LymphocytesCell DifferentiationImmunologic MemoryMechanistic Target of Rapamycin Complex 2Memory T CellsMiceMultiprotein ComplexesProtein Serine-Threonine KinasesSirolimusTOR Serine-Threonine KinasesConceptsT cell differentiationT cellsMemory T cell differentiationMemory precursor phenotypeMemory T cellsEfficacy of vaccinesGlucocorticoid-regulated kinase 1Mechanistic targetRapamycin complex 2 (mTORC2) signalingT cell metabolismT cells resultsKinase 1Memory CD8Acute infectionGlucocorticoid kinase 1Tumor immunotherapyCD8Cell differentiationPrecursor phenotypeNuclear translocationPowerful targetSerumRecall capacityEssential regulatorCell metabolism
2021
mTORC1 Signaling Regulates Proinflammatory Macrophage Function and Metabolism.
Collins S, Oh M, Sun I, Chan-Li Y, Zhao L, Powell J, Horton M. mTORC1 Signaling Regulates Proinflammatory Macrophage Function and Metabolism. The Journal Of Immunology 2021, 207: 913-922. PMID: 34290107, DOI: 10.4049/jimmunol.2100230.Peer-Reviewed Original ResearchMeSH KeywordsAcetylationAnimalsCells, CulturedCellular ReprogrammingCytokinesInflammationMacrophagesMechanistic Target of Rapamycin Complex 1Mechanistic Target of Rapamycin Complex 2MiceMice, Inbred C57BLSignal TransductionSirtuinsTh1 CellsConceptsKey regulatorImmune cell functionEnhanced histone acetylationCell functionRapid energy sourceClass III histoneDifferentiation of macrophagesHistone acetylationMacrophage functionMTORC1 signalingCellular metabolismOxidative phosphorylationCell metabolismMTOR signalingGlycolytic metabolismAntimicrobial compoundsGenetic deletionM2 macrophagesMouse macrophagesProper wound healingMetabolic programmingSignificant defectsM1 functionImmune cell metabolismSignaling
2017
mTORC2 Signaling Selectively Regulates the Generation and Function of Tissue-Resident Peritoneal Macrophages
Oh M, Collins S, Sun I, Tam A, Patel C, Arwood M, Chan-Li Y, Powell J, Horton M. mTORC2 Signaling Selectively Regulates the Generation and Function of Tissue-Resident Peritoneal Macrophages. Cell Reports 2017, 20: 2439-2454. PMID: 28877476, PMCID: PMC5659290, DOI: 10.1016/j.celrep.2017.08.046.Peer-Reviewed Original ResearchConceptsTissue-resident macrophagesMetabolic reprogrammingTissue-specific cuesUnique differentiation programCritical roleDifferentiation programmingPeritoneal resident macrophagesDifferentiation programMTORC2 activationGATA6 expressionPeritoneal macrophagesResident peritoneal macrophagesTissue microenvironmentHomeostatic functionsReprogrammingSelective roleMacrophage generationMacrophage proliferationDifferentiationDependent fashionResident macrophagesMacrophagesM2 macrophagesGATA6Microenvironment
2015
mTORC1 and mTORC2 selectively regulate CD8+ T cell differentiation
Pollizzi K, Patel C, Sun I, Oh M, Waickman A, Wen J, Delgoffe G, Powell J. mTORC1 and mTORC2 selectively regulate CD8+ T cell differentiation. Journal Of Clinical Investigation 2015, 125: 2090-2108. PMID: 25893604, PMCID: PMC4463194, DOI: 10.1172/jci77746.Peer-Reviewed Original ResearchMeSH KeywordsAdoptive TransferAnimalsCarrier ProteinsCD4-CD8 RatioCD8-Positive T-LymphocytesCell Line, TumorDeoxyglucoseFemaleGenes, ReporterGlycolysisImmunologic MemoryInterferon-gammaLymphocyte ActivationLymphopoiesisMaleMechanistic Target of Rapamycin Complex 1Mechanistic Target of Rapamycin Complex 2Melanoma, ExperimentalMiceMice, CongenicMice, Inbred C57BLMonomeric GTP-Binding ProteinsMultiprotein ComplexesNeuropeptidesOvalbuminPeptide FragmentsPhosphorylationProtein Processing, Post-TranslationalProto-Oncogene Proteins c-aktRapamycin-Insensitive Companion of mTOR ProteinRas Homolog Enriched in Brain ProteinRecombinant Fusion ProteinsSirolimusThymomaTOR Serine-Threonine KinasesTransduction, GeneticTumor Necrosis Factor-alphaConceptsGeneration of CD8T cell effectorsT cellsTuberous sclerosis complex 2Cell effectorsT cell effector responsesMemory-like T cellsEffector cell subsetsT cell memoryDifferentiation of CD4T cell-specific deletionT cell differentiationCell-specific deletionSurface marker expressionMTOR-dependent pathwayEvaluation of miceEffector CD8Antitumor immunityEffector phenotypeEffector cellsCell subsetsRecall responsesVaccine efficacyEffector responsesCD8
2014
The AGC kinase SGK1 regulates TH1 and TH2 differentiation downstream of the mTORC2 complex
Heikamp E, Patel C, Collins S, Waickman A, Oh M, Sun I, Illei P, Sharma A, Naray-Fejes-Toth A, Fejes-Toth G, Misra-Sen J, Horton M, Powell J. The AGC kinase SGK1 regulates TH1 and TH2 differentiation downstream of the mTORC2 complex. Nature Immunology 2014, 15: 457-464. PMID: 24705297, PMCID: PMC4267697, DOI: 10.1038/ni.2867.Peer-Reviewed Original ResearchMeSH KeywordsAdaptive ImmunityAnimalsAsthmaCell DifferentiationCells, CulturedEndosomal Sorting Complexes Required for TransportGene Expression RegulationHepatocyte Nuclear Factor 1-alphaImmediate-Early ProteinsInterferon-gammaMechanistic Target of Rapamycin Complex 2Melanoma, ExperimentalMiceMice, Inbred C57BLMice, KnockoutMultiprotein ComplexesNedd4 Ubiquitin Protein LigasesPoxviridae InfectionsProtein Serine-Threonine KinasesT Cell Transcription Factor 1Th1 CellsTh2 CellsTOR Serine-Threonine KinasesTranscription FactorsTumor BurdenUbiquitin-Protein LigasesVaccinia virus