High resolution melting: a useful field-deployable method to measure dhfr and dhps drug resistance in both highly and lowly endemic Plasmodium populations
Ndiaye YD, Diédhiou CK, Bei AK, Dieye B, Mbaye A, Mze NP, Daniels RF, Ndiaye IM, Déme AB, Gaye A, Sy M, Ndiaye T, Badiane AS, Ndiaye M, Premji Z, Wirth DF, Mboup S, Krogstad D, Volkman SK, Ahouidi AD, Ndiaye D. High resolution melting: a useful field-deployable method to measure dhfr and dhps drug resistance in both highly and lowly endemic Plasmodium populations. Malaria Journal 2017, 16: 153. PMID: 28420422, PMCID: PMC5395743, DOI: 10.1186/s12936-017-1811-2.Peer-Reviewed Original ResearchConceptsMalaria endemic sitesDrug resistanceHigh prevalenceUncomplicated Plasmodium falciparum malariaPolymerase chain reaction-restriction fragment length polymorphism methodologyAnti-malarial drug usePlasmodium falciparum malariaEndemic sitesEmergence of resistanceMultiplicity of infectionFalciparum malariaFragment length polymorphism methodologyDHFR mutationsBlood samplesMalaria endemicityPCR-RFLP genotypingPCR/RFLPTreatment policyCodon 540Drug usePlasmodium populationsMixed allelesEarly detectionCodons 51Mixed infections